Development of a sensitive surface plasmon resonance immunosensor for detection of 2,4-dinitrotoluene with a novel oligo (ethylene glycol)-based sensor surface

Nagatomo, Kazutaka; Kawaguchi, Toshikazu; Miura, Norio; Toko, Kiyoshi; Matsumoto, Kiyoshi

doi:10.1016/j.talanta.2009.02.018

Cited by 46 publications

(36 citation statements)

References 37 publications

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“…DSU has a long straight alkyl chain and a succinimidyl group in the head group; it forms a highly oriented and concentrated monolayer on Au surfaces [27]. CEG 6 is widely used as a biochemical linker because of its ability to prevent nonspecific adsorption [28][29][30][31]. C 2 -NTA is of interest to researchers because of its well-defined molecular structure and interesting properties, such as low intrinsic viscosity and solubility higher than analogues linear molecules [32].…”

Section: Introductionmentioning

confidence: 99%

Effect of alkanethiol molecular structure on sensitivity of surface plasmon resonance sensor

Suherman

Morita

Kawaguchi

2015

Sensors and Actuators B: Chemical

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This work aims to find the key factor determining the detection limit for an indirect competitive inhibition immunoassay using surface plasmon resonance (SPR) sensing. In our previous work, the thiol solution concentration used in a self-assembly process highly affected the alkanethiol monolayer structure on the sensor surface [Suherman et al., 2014]. It was noticed that the monolayer structure determined the immunoassay sensitivity due to the orientation and the surface concentration of antigen in domain structure of monolayer. To study the effect of orientation of antigen, we examined here three types of alkanethiol compounds: dithiobis(succinimidyl undecanoate) (DSU; straight-chain alkanethiol), carboxy-EG 6 -undecanethiol (CEG 6 ; flexible-chain alkanethiol), and(C 2 -NTA; three-branched alkanethiol). By electrochemical reductive desorption, the surface concentration of DSU, CEG 6 , and C 2 -NTA were estimated to be 6.6 × 10 -10 , 8.5 × 10 -10 , 4.6 × 10 -10 mol cm -2 , respectively. Subsequently, SPR suggested that the ratios of immobilized antigen (clenbuterol) per thiol were 0.13, 0.16, and 0.15 for DSU, CEG 6 , and C 2 -NTA, respectively. This suggests that the amount of immobilized clenbuterol was defined by the molecular size of clenbuterol. Sensor surface structures on a molecular scale were evaluated using high-resolution scanning tunneling microscopy (STM), which showed tilted clenbuterol with θ DSU > θ CEG6 > θ C2-NTA . For SPR sensing of clenbuterol, C 2 -NTA showed the highest sensitivity (LOD = 10 ppt) among the examined alkanethiols, because clenbuterol formed a laid-on structure. Based on kinetics, the key factor for sensing performance is discussed.2

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Section: Introductionmentioning

confidence: 99%

Effect of alkanethiol molecular structure on sensitivity of surface plasmon resonance sensor

Suherman

Morita

Kawaguchi

2015

Sensors and Actuators B: Chemical

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“…However, the development process is challenging owing to the lack of an explosivesensing material compatible with field application. (2) Much effort has been made to develop reliable direct explosive detection methods, such as bioreceptor-based sensing, (3,4) synthetic-material-based sensing, (5)(6)(7)(8) and Meisenheimer-complex-based sensing. (9) However, the bioreceptor and the Meisenheimer complex approaches have a sensitivity issue under a dry condition.…”

Section: Introductionmentioning

confidence: 99%

Gas-Phase Nitroaromatic Detection Based on Sulfonated Tetrafluoroethylene and Its Field Application

2014

Sensors and Materials

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We report on the use of sulfonated tetrafluoroethylene (ST) for explosive sensing and its feasibility for field application. The sensing performance of ST is verified with a quartz crystal microbalance (QCM) setup and a certified standard gas generator. ST can directly capture 2,4-dinitrotoluene (DNT), an indicator molecule of explosives, from the gas. Unlike other newly developed technologies for explosive detection, the use of ST does not need a humid or aqueous environment, which is difficult to maintain in field application. A handheld explosive detector is developed on the basis of ST and a miniaturized QCM system. Its sensitivity is −33.52 Hz/ppb and its limit of detection (LOD) is 1.19 ppb. Furthermore, a semifield test is performed with the device and a field-reproducing setup. This work successively demonstrates the explosive detection capability in an uncontrolled field environment.

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“…(2,3) In addition, we developed three kinds of sensor surfaces that are highly sensitive for the detection of nitroaromatic compounds. (4)(5)(6) However, the detection time was over 15 min in those studies, because we chose an indirect competitive assay format for highly sensitive detection of explosives. In the assay format, the antibody and antigen solutions are premixed and incubated for about 15 min before measurement.…”

Section: Introductionmentioning

confidence: 99%

Displacement Immunosensor Based on Surface Plasmon Resonance for Rapid and Highly Sensitive Detection of 2,4,6-Trinitrotoluene

Onodera¹,

Mizuta

Kazuhiko

et al. 2011

Sensors and Materials

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By making use of the antigen-antibody interaction and a surface plasmon resonance (SPR) sensor, we developed a measurement procedure of displacement immunoassay to rapidly detect 2,4,6-trinitrotoluene (TNT). In this procedure, TNT solutions were injected in 30 s at the end of flowing an anti-TNT antibody. Three kinds of sensor surfaces were modified with TNT analogues, namely, TNP-glycine, DNP-glycine, and DNP-acetic acid in a self-assembled monolayer containing ethylene glycol. We investigated which of the TNT analogues provided higher sensitivity using the displacement immunosensor. As a result, the limit of detection (LOD) of TNT was 0.4 ng/mL (ppb) when using the DNP-glycine-modified Au sensor surface with a oneminute flow of TNT solution. We concluded that the final TNT LOD was 0.9 ppb on the basis of experiments using the three different DNP-glycine-modified sensor surfaces. The LOD was 0.7 ppb when using the sensorgram slope 10 s after TNT injection. The displacement immunosensor can detect TNT at sub-ppb levels in 12 s.

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Development of a sensitive surface plasmon resonance immunosensor for detection of 2,4-dinitrotoluene with a novel oligo (ethylene glycol)-based sensor surface

Cited by 46 publications

References 37 publications

Effect of alkanethiol molecular structure on sensitivity of surface plasmon resonance sensor

Effect of alkanethiol molecular structure on sensitivity of surface plasmon resonance sensor

Gas-Phase Nitroaromatic Detection Based on Sulfonated Tetrafluoroethylene and Its Field Application

Displacement Immunosensor Based on Surface Plasmon Resonance for Rapid and Highly Sensitive Detection of 2,4,6-Trinitrotoluene

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