Development of a Simple Enzymatic Method for Screening Sucrose Content in Legume Seeds

Kim, Gyu-Tae; Park, Aron; Kim, Woon Ji; Moon, Chang Yeok; Kang, Byeong Hee; Kim, Seong Hoon; Choi, Yu-Mi; Ha, Bo‐Keun

doi:10.9787/pbb.2021.9.3.250

Plant Breed. Biotech.

2021

DOI: 10.9787/pbb.2021.9.3.250

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Development of a Simple Enzymatic Method for Screening Sucrose Content in Legume Seeds

Gyu-Tae Kim

Aron Park

Woon Ji Kim

et al.

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2024

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Cited by 1 publication

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Screening Germplasms and Detecting Quantitative Trait Loci for High Sucrose Content in Soybean

Kang,

Shin,

Kang

et al. 2024

Plants

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Sucrose is a desirable component of processed soybean foods and animal feed, and thus, its content is used as an important characteristic for assessing the quality of soybean seeds. However, few studies have focused on the quantitative trait loci (QTLs) associated with sucrose regulation in soybean seeds. This study aims to measure the sucrose content of 1014 soybean accessions and identify genes related to high sucrose levels using QTL analysis. Colorimetric analysis based on the enzymatic reaction of invertase (INV) and glucose oxidase (GOD) was employed to test the germplasms. A total of six high-sucrose genetic resources (IT186230, IT195321, IT263138, IT263276, IT263286, and IT276521) and two low-sucrose genetic resources (IT025668 and IT274054) were identified. Two F2:3 populations, IT186230 × IT025668 and Ilmi × IT186230, were then established from these germplasms. QTL analysis identified four QTLs (qSUC6.1, qSUC11.1, qSUC15.1, and qSUC17.1), explaining 7.3–27.6% of the phenotypic variation in the sugar content. Twenty candidate genes were found at the four QTLs. Notably, Glyma.17G152300, located in the qSUC17.1 QTL region, exhibited a 17-fold higher gene expression in the high-sucrose germplasm IT186230 compared to the control germplasm Ilmi, confirming its role as a major gene regulating the sucrose content in soybean. These results may assist in marker-assisted selection for breeding programs that aim to develop soybean lines with a higher sucrose content.

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Screening Germplasms and Detecting Quantitative Trait Loci for High Sucrose Content in Soybean

Kang,

Shin,

Kang

et al. 2024

Plants

View full text Add to dashboard Cite

show abstract

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Development of a Simple Enzymatic Method for Screening Sucrose Content in Legume Seeds

Cited by 1 publication

References 25 publications

Screening Germplasms and Detecting Quantitative Trait Loci for High Sucrose Content in Soybean

Screening Germplasms and Detecting Quantitative Trait Loci for High Sucrose Content in Soybean

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