Development of a simple, low-cost and eurytopic medium based on Pleurotus eryngii for lactic acid bacteria

Abstract

Lactic acid bacteria (LAB) are a group of important beneficial microorganisms for human, but their growth is restricted to the habitats with rich nutrients. In order to develop a simple, low-cost and efficient medium based on the mushroom Pleurotus eryngii, this study evaluated the effects of different treatment methods for the mushroom, concentration of the mushroom, buffers, tween 80, MgSO 4 ·7H 2 O, MnSO 4 ·4H 2 O, CuSO 4 ·5H 2 O, riboflavin and ascorbic acid on the growth of Lactococcus lactis subsp. lactis SLPE1-3. An optimized medium was developed, which was composed of the mushroom at 200 g/L, the buffer sodium acetate at 5 g/L, and riboflavin at 0.5 mg/L. The mushroom was ground, boiled and filtered for the filtrate in advance. In this optimized medium which was named as PSR medium, the population density of SLPE1-3 sharply reached 2.13 × 10 9 CFU/mL within 18 h of incubation, and still maintained 1.17 × 10 8 CFU/mL at 120 h. In addition, this study found that 6 kinds of LAB could grow almost well, and maintained high survival in PSR medium compared to M17 or MRS medium, including Lactococcus lactis subsp. lactis, Lactobacillus plantarum, Lactococcus lactis subsp. cremoris, Lactobacillus paracasei, Pediococcus pentosaceus and Lactobacillus rhamnosus. These results showed that PSR medium was a simple, low cost and eurytopic medium for the cultivation of LAB, and could replace MRS or M17 medium in the food industry, biomedicine and laboratory.

Lactic Acid Bacteria of Marine Origin as a Tool for Successful Shellfish Farming and Adaptation to Climate Change Conditions

“…Throughout their evolutionary journey, LAB have undergone genetic changes resulting in the loss of genes responsible for cofactor synthesis and the biosynthetic pathways for certain amino acids and vitamins. Consequently, LAB require nutrient-rich media for their growth and development [13].…”

Development of Cabbage Juice Medium for Industrial Production of Leuconostoc mesenteroides Starter

“…In addition, the bacterial productivity in the medium should be increased by optimizing the medium component and its concentrations [11]. Recently, media using vegetables and grains such as carrots or buckwheats were used for culturing vegetablederived LAB [12]. However, very little effort has been made to optimize these agricultural products as economical media for mass production of bacterial culture [13].…”