Development of a Sri Lankan rice variety Bg 94-1 harbouring cry2A gene of Bacillus thuringiensis resistant to rice leaffolder  [Cnaphalocrocis medinalis (Guenée)]

Gunasekara, Jma; Jayasekera, G.A.U.; Perera, K. F. G.; Wickramasuriya, AM

doi:10.4038/jnsfsr.v45i2.8180

Cited by 9 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Rice lines (var. Bg94–1) produced by transferring the insecticidal protein cry2A cause mortality in rice leaf folders in 80% of cases ( Gunasekara et al, 2017 ). Similarly, transgenic pigeon pea lines constructed using a combination of cry1Ac and cry2Aa exhibited resistance to H. armigera , resulting in 80%–100% larval mortality ( Ghosh et al, 2017 ).…”

Section: Biotechnological Approaches In Insect Pest Managementmentioning

confidence: 99%

Biotechnological Approaches for Host Plant Resistance to Insect Pests

et al. 2022

View full text Add to dashboard Cite

Annually, the cost of insect pest control in agriculture crosses billions of dollars around the world. Until recently, broad-spectrum synthetic pesticides were considered as the most effective means of pest control in agriculture. However, over the years, the overreliance on pesticides has caused adverse effects on beneficial insects, human health and the environment, and has led to the development of pesticide resistant insects. There is a critical need for the development of alternative pest management strategies aiming for minimum use of pesticides and conservation of natural enemies for maintaining the ecological balance of the environment. Host plant resistance plays a vital role in integrated pest management but the development of insect-resistant varieties through conventional ways of host plant resistance takes time, and is challenging as it involves many quantitative traits positioned at various loci. Biotechnological approaches such as gene editing, gene transformation, marker-assisted selection etc. in this direction have recently opened up a new era of insect control options. These could contribute towards about exploring a much wider array of novel insecticidal genes that would otherwise be beyond the scope of conventional breeding. Biotechnological interventions can alter the gene expression level and pattern as well as the development of transgenic varieties with insecticidal genes and can improve pest management by providing access to novel molecules. This review will discuss the emerging biotechnological tools available to develop insect-resistant engineered crop genotypes with a better ability to resist the attack of insect pests.

show abstract

Section: Biotechnological Approaches In Insect Pest Managementmentioning

confidence: 99%

Biotechnological Approaches for Host Plant Resistance to Insect Pests

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Transgene Resistance to leaf folder [128] Mannose-specific GNA Transgene Resistance toBPH (Nilaparvata lugens) and hemipteran pest [127] Cry1AC + ASAL Transgene Resistance to yellow stem borer, leaf folder, and BPH [129] Cry1Ab + vip3A Transgene Resistance toAsian stem borer and rice leaf folder [130] Cry2AX1 Transgene Resistance to rice leaf folder [131] Cry2Aa + cry1Ca…”

Section: Cry2amentioning

confidence: 99%

CRISPR-Cas Genome Editing for Insect Pest Stress Management in Crop Plants

et al. 2022

View full text Add to dashboard Cite

Global crop yield and food security are being threatened by phytophagous insects. Innovative methods are required to increase agricultural output while reducing reliance on hazardous synthetic insecticides. Using the revolutionary CRISPR-Cas technology to develop insect-resistant plants appears to be highly efficient at lowering production costs and increasing farm profitability. The genomes of both a model insect, Drosophila melanogaster, and major phytophagous insect genera, viz. Spodoptera, Helicoverpa, Nilaparvata, Locusta, Tribolium, Agrotis, etc., were successfully edited by the CRISPR-Cas toolkits. This new method, however, has the ability to alter an insect’s DNA in order to either induce a gene drive or overcome an insect’s tolerance to certain insecticides. The rapid progress in the methodologies of CRISPR technology and their diverse applications show a high promise in the development of insect-resistant plant varieties or other strategies for the sustainable management of insect pests to ensure food security. This paper reviewed and critically discussed the use of CRISPR-Cas genome-editing technology in long-term insect pest management. The emphasis of this review was on the prospective uses of the CRISPR-Cas system for insect stress management in crop production through the creation of genome-edited crop plants or insects. The potential and the difficulties of using CRISPR-Cas technology to reduce pest stress in crop plants were critically examined and discussed.

show abstract

“…In 1987, Vaeck et al successfully introduced the cry1A(b) gene into tobacco through Agrobacterium -mediated transformation and obtained stem borer-resistant transgenic tobacco [ 10 ]. Subsequently, the Bt gene was introduced into crops such as cotton [ 11 , 12 , 13 , 14 ], maize [ 15 ], rice [ 16 , 17 , 18 , 19 ], and tomato [ 20 ], resulting in effective improvement in stem borer resistance. In sugarcane, Arencibia et al first described the transformation of cry1A gene to improve stem borer resistance in 1997 [ 8 ], followed by numerous reports on the improvement of insect resistance in transgenic sugarcanes such as cry1A(b) [ 21 , 22 , 23 ], GNA [ 24 , 25 ], cry1Aa3 [ 26 ], cry1Ac [ 27 , 28 , 29 ] and proteinase inhibitor [ 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

Particle Bombardment of the cry2A Gene Cassette Induces Stem Borer Resistance in Sugarcane

Gao

Yang

et al. 2018

IJMS

View full text Add to dashboard Cite

Sugarcane borer is the most common and harmful pest in Chinese sugarcane fields, and can cause damage to the whole plant during the entire growing season. To improve borer resistance in sugarcane, we constructed a plant expression vector pGcry2A0229 with the bar gene as the marker and the cry2A gene as the target, and introduced it into embryogenic calli of most widely cultivated sugarcane cultivar ROC22 by particle bombardment. After screening with phosphinothricin in vitro and Basta spray, 21 resistance-regenerated plants were obtained, and 10 positive transgenic lines harboring the cry2A gene were further confirmed by conventional PCR detection. Real-time quantitative PCR (RT-qPCR) analysis showed that the copy number of the cry2A gene varied among different transgenic lines but did not exceed four copies. Quantitative ELISA analysis showed that there was no linear relationship with copy number but negatively correlated with the percentage of borer-infested plants. The analysis of industrial and agronomic traits showed that the theoretical sugar yields of transgenic lines TR-4 and TR-10 were slightly lower than that of the control in both plant cane and ratoon cane; nevertheless, TR-4 and TR-10 lines exhibited markedly lower in frequency of borer-infested plants in plant cane and in the ratoon cane compared to the control. Our results indicate that the introduction of the cry2A gene via bombardment produces transgenic lines with obviously increased stem borer resistance and comparable sugar yield, providing a practical value in direct commercial cultivation and crossbreeding for ROC22 has been used as the most popular elite genitor in various breeding programs in China.

show abstract

Development of a Sri Lankan rice variety Bg 94-1 harbouring cry2A gene of Bacillus thuringiensis resistant to rice leaffolder [Cnaphalocrocis medinalis (Guenée)]

Cited by 9 publications

References 0 publications

Biotechnological Approaches for Host Plant Resistance to Insect Pests

Biotechnological Approaches for Host Plant Resistance to Insect Pests

CRISPR-Cas Genome Editing for Insect Pest Stress Management in Crop Plants

Particle Bombardment of the cry2A Gene Cassette Induces Stem Borer Resistance in Sugarcane

Contact Info

Product

Resources

About