2005
DOI: 10.1016/j.vaccine.2005.04.045
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Development of a synthetic consensus sequence scrambled antigen HIV-1 vaccine designed for global use

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Cited by 38 publications
(22 citation statements)
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“…The SAVINE vaccine was designed and constructed by using methods previously used to construct a human immunodeficiency virus SAVINE (47). The SAVINE incorporates the LMP1 sequence from the Cao isolate of EBV identified from a Chinese NPC (GenBank accession number AF304432), while the LMP2a and EBNA1 protein sequences were obtained from a common EBV A strain sequence assembled from EBV B95-8 (GenBank accession number AJ507799).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…The SAVINE vaccine was designed and constructed by using methods previously used to construct a human immunodeficiency virus SAVINE (47). The SAVINE incorporates the LMP1 sequence from the Cao isolate of EBV identified from a Chinese NPC (GenBank accession number AF304432), while the LMP2a and EBNA1 protein sequences were obtained from a common EBV A strain sequence assembled from EBV B95-8 (GenBank accession number AJ507799).…”
Section: Methodsmentioning
confidence: 99%
“…The SAVINE cDNA was synthesized by using long oligonucleotides from Invitrogen, asymmetric PCR, stepwise overlap extension, and restriction sitedirected ligation as described previously (47) to make a 6,863-bp cDNA fragment (Fig. 1).…”
Section: Methodsmentioning
confidence: 99%
“…There have been numerous T-cell-based vaccine strategies, including the following: (i) the linkage of prominent major histocompatibility complex (MHC) class I-and class II-restricted T-cell targets (26), (ii) the use of viral proteins or fusion proteins carrying viral peptides (24), (iii) the insertion of peptides or proteins into DNA or viral vectors (9), (iv) the synthesis of ancestral or consensus sequences (22), and (v) the scrambling of peptides from more than one viral protein (31). The preferred outcome is that T cells will reactivate in the context of a sequence-similar infectious HIV-1 challenge.…”
mentioning
confidence: 99%
“…A popular suggestion in the HIV-1 vaccine field has been that one or a few consensus or ancestral HIV-1 sequences may be sufficient to elicit protective T-cell responses toward diverse HIV-1 isolates (11,14,31). Such suggestions rely on the supposition that vaccine sequences, if matched or partially matched in the challenge virus, will be readily processed and presented to T-cell populations.…”
mentioning
confidence: 99%
“…This gene was small in comparison to other sequences generated by SOE-PCR (Cello et al, 2002;Kodumal et al, 2004;Thomson et al, 2005;Gibson et al, 2008;Bustami et al, 2009). …”
Section: Discussionmentioning
confidence: 76%