Development of a test strip for rapid detection of Gymnodinium catenatum

Abstract

Harmful algal blooms (HABs) are major ecological and environmental problems in China's coastal waters and seriously threaten the stability of the marine ecosystem and human health. Gymnodinium catenatum is a toxic red tide dino agellate. It can produce paralytic shell sh toxins (PSP), which cause serious hazards to marine organisms, public health and safety. In this paper, a test strip based on colloidal gold immunochromatography (GICG) was developed for the rapid detection of Gymnodinium catenatum. The experimental results showed that the test trip has good speci city and sensitivity. It not only detects the different components of Gymnodinium catenatum but also may detect algal toxins. The lowest density of Gymnodinium catenatum that can be detected by this test strip is approximately 120 cells/mL. Crossreaction indicated that the test strip had a high speci city for Gymnodinium catenatum. This test strip provides a rapid method for on-site detection of Gymnodinium catenatum and a reference method for the monitoring of other harmful algae to serve as an early warning of upcoming red tides. It also provides a new way to prepare more detection methods for toxic algal toxins.

A gold-based immunochromatographic strip for the specific detection of tacrolimus in whole blood

“…29 The immunoassay is simple to perform and low cost, but it has the disadvantages of low sensitivity and lack of specificity. 30 In recent years, the colloidal gold-based immunochromatographic strip (CG-ICS), as a low-cost, high-throughput, timesaving, easy-to-operate and portable biosensor, has been widely applied in areas of food safety, 31 environmental pollution, 32 COVID-19 control 33 and clinical research. 34 The CG-ICS visualizes the specific combination of antigen and antibody through the optical properties of colloidal gold nanoparticles.…”