Development of a time-resolved fluoroimmunoassay for salmon insulin-like growth factor binding protein-1b

Fukuda, Miki; Kaneko, Nobuto; Kawaguchi, Kohei; Hevrøy, Ernst M.; Hara, Akihiko; Shimizu, Munetaka

doi:10.1016/j.cbpa.2015.04.016

Cited by 15 publications

(17 citation statements)

References 56 publications

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“…Time-resolved fluorescence was measured using Wallac ARVO SX (PerkinElmer) at 615 nm. It measured IGFBP-1b levels as low as 0.4 ng/ml, and ED80 and ED20 were 0.9 and 24.6 ng/ml, respectively; The intra-and inter-assay coefficients of variation were 0.7% and 12.5%, respectively; The TR-FIA did not cross-react with the 41-kDa form (IGFBP-2b) but showed 3% cross-reactivity with the 28-kDa form (IGFBP-1a) (Fukuda et al, 2015).…”

Section: Time-resolved Fluoroimmunoassay (Tr-fia) For Igfbp-1bmentioning

confidence: 97%

“…Plasma IGFBP-1b level was measured by a newly developed TR-FIA (Fukuda et al, 2015). Briefly, plasma samples were first incubated with antiserum against purified salmon IGFBP-1b (Shimizu et al, 2006) at a dilution of 1:5000 overnight at 4°C in a 96-well microtiter plate coated with goat anti-rabbit IgG (PerkinElmer).…”

Section: Time-resolved Fluoroimmunoassay (Tr-fia) For Igfbp-1bmentioning

confidence: 99%

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Role of the GH-IGF-1 system in Atlantic salmon and rainbow trout postsmolts at elevated water temperature

Hevrøy¹,

Tipsmark

Remø³

et al. 2015

Comparative Biochemistry and Physiology Part A: Molecular &

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Section: Time-resolved Fluoroimmunoassay (Tr-fia) For Igfbp-1bmentioning

confidence: 97%

Section: Time-resolved Fluoroimmunoassay (Tr-fia) For Igfbp-1bmentioning

confidence: 99%

Role of the GH-IGF-1 system in Atlantic salmon and rainbow trout postsmolts at elevated water temperature

Hevrøy¹,

Tipsmark

Remø³

et al. 2015

Comparative Biochemistry and Physiology Part A: Molecular &

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“…A negative relationship between plasma IGFBP-1b and condition factor is generally found in salmon, suggesting that this form is involved in catabolism (Shimizu et al, 2006). In addition, serum IGFBP-1b levels in smolting masu salmon were positively correlated with gill NKA activity (Fukuda et al, 2015). During smoltification, circulating IGF-I in smolting salmon are generally high and both growth and hypo-osmoregulatory ability are promoted (Dickhoff et al, 1997;Stefansson et al, 2008;Björnsson et al, 2011;McCormick, 2013).…”

Section: Developmental/seasonal Effects (Smoltification)mentioning

confidence: 99%

Circulating insulin-like growth factor binding proteins in fish: Their identities and physiological regulation

Shimizu

Dickhoff

2017

General and Comparative Endocrinology

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Insulin-like growth factor binding proteins (IGFBPs) play crucial roles in regulating the availability of IGFs to receptors and prolong the half-lives of IGFs. There are six IGFBPs present in the mammalian circulation with IGFBP-3 being most abundant. In mammals IGFBP-3 is the major carrier of circulating IGFs, facilitated by forming a ternary complex with IGF and an acid-labile subunit (ALS). IGFBP-1 is generally inhibitory to IGF action by preventing it from interacting with its receptors. In teleosts, the third-round of vertebrate whole genome duplication created paralogs of each IGFBP, except IGFBP-4. In the fish circulation, three major IGFBPs are typically detected at molecular ranges of 20-25, 28-32 and 40-50kDa. However, their identities are not well established. Three major circulating IGFBPs in Chinook salmon have been identified through protein purification and cDNA cloning. Salmon 28- and 22-kDa IGFBPs are co-orthologs of IGFBP-1, termed IGFBP-1a and -1b, respectively. They are induced under catabolic conditions such as stress and fasting but their responses are somewhat different, with IGFBP-1b being the most sensitive of the two. Cortisol stimulates production and secretion of these IGFBP-1 subtypes while, unlike in mammals, insulin may not be a primary suppressor. Salmon 41-kDa IGFBP, a major carrier of IGF-I, is not IGFBP-3, as might be expected extrapolating from mammals, but is in fact IGFBP-2b. Salmon IGFBP-2b levels in plasma are high when fish are fed, and GH treatment increases its circulating levels similar to mammalian IGFBP-3. These findings suggest that salmon IGFBP-2b acquired the role and regulation similar to mammalian IGFBP-3. Multiple replications of fish IGFBPs offer a unique opportunity to investigate molecular evolution of IGFBPs.

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“…In addition, a negative correlation between circulating IGFBP-1b and individual growth rate was reported in post-smolt coho salmon (Shimizu et al, 2006). We recently established a time-resolved fluoroimmunoassay (TR-FIA) for salmon IGFBP-1b using the same components to those for radioimmunoassay (Fukuda et al, 2015). With this TR-FIA, we discovered that IGFBP-1b showed a negative relationship with growth rate in yearling and underyearling masu salmon (O. masou) (Kawaguchi et al, 2013;Fukuda et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…We recently established a time-resolved fluoroimmunoassay (TR-FIA) for salmon IGFBP-1b using the same components to those for radioimmunoassay (Fukuda et al, 2015). With this TR-FIA, we discovered that IGFBP-1b showed a negative relationship with growth rate in yearling and underyearling masu salmon (O. masou) (Kawaguchi et al, 2013;Fukuda et al, 2015). Thus, circulating IGFBP-1b may be useful as a negative growth index in juvenile salmon in the wild.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of growth status using endocrine growth indices, insulin-like growth factor (IGF)-I and IGF-binding protein-1b, in out-migrating juvenile chum salmon

Kaneko

Torao

Koshino

et al. 2019

General and Comparative Endocrinology

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This study aimed to utilize circulating insulin-like growth factor binding protein (IGFBP)-1b as a negative index of growth to evaluate the growth status of juvenile chum salmon (Oncorhynchus keta) in the ocean. First, rearing experiments using PIT-tagged juveniles were conducted to examine the relationship of circulating IGFBP-1b with growth rate of the fish in May and in June. The serum IGFBP-1b level negatively correlated with fish growth rate in both months, suggesting its utility as a negative index of growth. Next, the growth status of outmigrating juveniles in northeastern Hokkaido, Japan, was monitored for 3 years using the growth indices. Serum levels of IGF-I, a positive index of growth, in fish collected from the nearshore zone were low in May and high in June of all years. Levels of serum IGFBP-1b showed a trend opposite to that of serum IGF-I. However, the IGF-I/IGFBP-1b molar ratios well reflected the seasonal and regional trends. These findings suggest that the juveniles in June left the nearshore area under better growth conditions. The present study also suggests that the use of multiple growth indices would improve the sensitivity and accuracy to evaluate the current growth status of out-migrating juvenile chum salmon.

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Development of a time-resolved fluoroimmunoassay for salmon insulin-like growth factor binding protein-1b

Cited by 15 publications

References 56 publications

Role of the GH-IGF-1 system in Atlantic salmon and rainbow trout postsmolts at elevated water temperature

Role of the GH-IGF-1 system in Atlantic salmon and rainbow trout postsmolts at elevated water temperature

Circulating insulin-like growth factor binding proteins in fish: Their identities and physiological regulation

Evaluation of growth status using endocrine growth indices, insulin-like growth factor (IGF)-I and IGF-binding protein-1b, in out-migrating juvenile chum salmon

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