Development of a versatile biotinylated material based on SU-8

Abstract

The negative epoxy-based SU-8 photoresist has a wide variety of applications within the semiconductor industry, photonics and lab-on-a-chip devices, and it is emerging as an alternative to silicon-based devices for sensing purposes. In the present work, biotinylation of the SU-8 polymer surface promoted by light is reported. As a result, a novel, effective, and low-cost material, focusing on the immobilization of bioreceptors and consequent biosensing, is developed. This material allows the spatial discrimination depending on the irradiation of desired areas. The most salient feature is that the photobiotin may be directly incorporated into the SU-8 curing process, consequently reducing time and cost. The potential use of this substrate is demonstrated by the immunoanalytical detection of the synthetic steroid gestrinone, showing excellent performances. Moreover, the naked eye biodetection due to the transparent SU-8 substrate, and simple instrumental quantification are additional advantages.

Development towards Compact Nitrocellulose-Based Interferometric Biochips for Dry Eye MMP9 Label-Free In-Situ Diagnosis

“…After that, the remains are eliminated with a PMMA industrial developer AR-600-55 (ALLRESIST GmbH, Strausberg, Germany) diluted 1:4 with propanol. When the BICELLs were manufactured chip by chip, the cleaning process was exhaustive [ 11 ]. However, fabrication at the wafer level drastically improves the yield because the cleaning process is reduced in a single rising step with dimethyl sulfoxide (DMSO).…”

Antigen-Antibody Affinity for Dry Eye Biomarkers by Label Free Biosensing. Comparison with the ELISA Technique

“…The SU8 surface of the BICELLs was treated with sulfuric acid (95% for 10 s) in order to have a hydrophilic sensing surface. As a result of this treatment, the SU8 epoxy groups are opened and suitable to immobilize covalently the protein [ 13 ].…”

Protein Covalently Conjugated SU-8 Surface for the Enhancement of Mesenchymal Stem Cell Adhesion and Proliferation

“…3−7 Moreover, SU-8 has optimal chemical stability and transparent to visible light after cross-linking, which can be used as optical waveguides, 8 probes for microscopy, 9 MEMS, 10−12 and molds for microchip. 13 Previous studies have shown that SU-8 could be nontoxic and biocompatible after polymerization, 14,15 indicating its potential to serve as the substrate of bioanalytical micro-and nanodevices, 16 such as biosensors, 17 bioarrays, 18 and drug delivery vehicles. 19 However, the hydrophobic nature of SU-8 has limited its biological applications in practice, which causes low specific adsorption of probes, 20 poor surface wettability, 21,22 and limited cell attachment.…”

“…Additionally, SU-8 is able to produce microstructure with high aspect ratio with a wide range of structure thickness. − Moreover, SU-8 has optimal chemical stability and transparent to visible light after cross-linking, which can be used as optical waveguides, probes for microscopy, MEMS, − and molds for microchip . Previous studies have shown that SU-8 could be nontoxic and biocompatible after polymerization, , indicating its potential to serve as the substrate of bioanalytical micro- and nanodevices, such as biosensors, bioarrays, and drug delivery vehicles …”