Development of acaricide resistance in Pacific spider mite (Tetranychus pacificus) from California vineyards

Abstract

In recent years, grape growers in California reported failures of acaricides against Tetranychus pacificus McGregor. We collected T. pacificus populations from four vineyards and tested them for resistance to bifenazate, propargite and pyridaben. In addition, we sequenced part of the cytochrome b gene of bifenazate-resistant and -susceptible T. pacificus to test for the presence of mutations reported to confer resistance to the congeneric T. urticae. None of the mutations conferring resistance to bifenazate in T. urticae were present in resistant T. pacificus. Resistance levels ranged from full susceptibility to statistically significant 11-fold resistance to pyridaben, sevenfold resistance to bifenazate and fourfold resistance to propargite compared to a susceptible population. Despite the relatively low levels of resistance detected, we estimated that under the conditions of our study the highest field rates of bifenazate and pyridaben application would cause less than 58 and 66% mortality of adult females in the most resistant populations, respectively. In contrast, field rates of propargite application would cause close to 100% mortality in the least susceptible population. These results highlight a potential link between resistance development and reduced field effectiveness for bifenazate and pyridaben. Finally, T. pacificus may be more tolerant to bifenazate and propargite than T. urticae, since the LC(50) values for the susceptible population of T. pacificus were several times higher than LC(50)'s reported for susceptible T. urticae.

Acaricide resistance mechanisms in the two-spotted spider mite Tetranychus urticae and other important Acari: A review

“…Table 1 Top 10 resistant arthropods, based on the number of unique active ingredients for which resistance has been reported and the number of cases reported (Whalon et al, 2008). Recently, the occurrence of resistant populations of these species has been reported (Miller et al, 2001;Auger et al, 2003;Aiki et al, 2005;Enayati et al, 2009;Stavrinides et al, 2010;Van Leeuwen et al, 2010), however, in very few cases attempts were made to study the resistance mechanism.…”

Acaricides - Biological Profiles, Effects and Uses in Modern Crop Protection

“…For instance, global popularity of METI-acaricides contributed to relatively fast development of resistant spider mite populations in Japan, South Korea, Australia, Brazil, California and some European countries. On the other hand, there are only few reports on resistance to fenbutatin-oxide and other organotin compounds which have been used for four decades now Auger et al, 2004;Stavrinides et al, 2010). Another phytophagous mite on the list of "top 20", bulb mite Rhizoglyphus robini (Acaridae), ranks 19 th with 22 reports on resistance to almost exclusively organophosphate compounds.…”

Ácaros asociados al maíz en México

“…La presencia de estos organismos tiene un impacto negativo en la producción y rendimiento. Para su control se han aplicado diferentes acaricidas, mismos que provocan serios problemas de resistencia, especialmente en especies del género Tetranychus (Stavrinides et al, 2010), por su uso continuo además de eliminar la fauna benéfica en el agroecosistema, lo cual ha generado que se busquen diferentes estrategias de control basadas principalmente en un sistema de Manejo Integrado de Plagas (Lomelí y Rodríguez, 2010;Rodríguez, 2012).…”