Development of Amino Acids Functionalized SBA-15 for the Improvement of Protein Adsorption

Abstract

Ordered mesoporous materials and their modification with multiple functional groups are of wide scientific interest for many applications involving interaction with biological systems and biomolecules (e.g., catalysis, separation, sensor design, nano-science or drug delivery). In particular, the immobilization of enzymes onto solid supports is highly attractive for industry and synthetic chemistry, as it allows the development of stable and cheap biocatalysts. In this context, we developed novel silylated amino acid derivatives (Si-AA-NH2) that have been immobilized onto SBA-15 materials in biocompatible conditions avoiding the use of toxic catalyst, solvents or reagents. The resulting amino acid-functionalized materials (SBA-15@AA) were characterized by XRD, TGA, EA, Zeta potential, nitrogen sorption and FT-IR. Differences of the physical properties (e.g., charges) were observed while the structural ones remained unchanged. The adsorption of the enzyme lysozyme (Lyz) onto the resulting functionalized SBA-15@AA materials was evaluated at different pHs. The presence of different functional groups compared with bare SBA-15 showed better adsorption results, for example, 79.6 nmol of Lyz adsorbed per m2 of SBA-15@Tyr compared with the 44.9 nmol/m2 of the bare SBA-15.

Repurposing Anthelmintics: Rafoxanide- and Copper-Functionalized SBA-15 Carriers against Methicillin-Resistant Staphylococcus aureus

“…Incorporating RFX into the SBA-15 matrix results in a 10-fold improvement in the RFX’s antibacterial property, likely because the SBA-15 matrix can help with the solubility of RFX in an aqueous solution. This is in line with some literature reports, where hydrophobic drugs have improved solubility when loaded into mesoporous materials. , …”

“…This is in line with some literature reports, where hydrophobic drugs have improved solubility when loaded into mesoporous materials. 66,67 To understand how the order of addition of the antimicrobial agents affects the bactericidal activity of the SBA-15 nanoparticles, release studies in PBS, using PBS 1×, are done at different pH values under simulated biological conditions (Figure 7). Ho et al did a similar experiment to understand the release of Ag(I) ions from surgical sutures, where the sutures were submerged in PBS for a number of days and the supernatant was later examined via atomic absorption spectroscopy (AAS).…”

Direct pseudomorphic transformation of silica from rice husk into organo-functionalized MCM-41

“…Three amide derivatives of amino-acid (AA) ( l -tyrosine (Tyr), l -serine (Ser), l -leucine (Leu)) triethoxysilanes (EtO 3 Si-R), named Si-Tyr-NH 2 , Si-Ser-NH 2 , Si-Leu-NH 2 (Scheme 1), were synthesized accordingly to the previously described protocol. 32 The molar weights are 427.57, 351.48, 377.56 g mol −1 , respectively. Acetonitrile (ACN) was purchased from Sigma-Aldrich.…”

“…Functionalization of ordered mesoporous silica, such as MCM-41 or SBA-15 materials, produce organic-inorganic hybrid silica materials 20,21,[26][27][28][29][30][31][32] of high surface area for a great variety of applications, as biotechnology, 27,33 catalysis and biocatalysis, 26,27 environmental remediation, 28,29 biosensors, 30 proteins separation, 34 high-performance liquid chromatography (HPLC) 22 etc. Two different pathways exist for mesoporous silica functionalization: post-grafting method with organo-trialkoxylsilanes, 22,32,35,36 or direct synthesis by co-condensation of tetraalkylorthosilicate with organo-trialkoxylsilanes followed by surfactants extraction with solvents. [37][38][39] The co-condensation method offers several advantages over the post-grafting method.…”

A bioinspired approach for the modulation of electroosmotic flow and protein–surface interactions in capillary electrophoresis using silylated amino‐amides blocks and covalent grafting

“…The amount of each Si–AA was determined to obtain a concentration between 1 and 1.2 mmol per g of SBA‐15 (510 mg of Si–TYR, 450 mg of Si–LEU, 420 mg of Si–SER, and 500 mg of Si–ARG). Those conditions were suitable to form a monolayer onto SBA‐15 surface [30]. Resulting products were washed by centrifugation three times with deionized water and once with ethanol.…”