Development of an Acellular Bioengineered Matrix with a Dominant Vascular Pedicle

Henderson, Peter W.; Nagineni, Vamsi V.; Harper, Alice; Bavinck, Nicola; Sohn, Allie M.; Krijgh, David D.; Jimenez, Natalia; Weinstein, Andrew L.; Spector, Jason A.

doi:10.1016/j.jss.2010.07.017

Cited by 29 publications

(22 citation statements)

References 16 publications

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“…Inhibition of venous outf low contributed to leakage and formation of edema. Our findings were inconsistent with a previous study by Henderson et al 16 which demonstrated that artery-vein circulation remained patency for 6 hours, by perfusing with a peristaltic perfusion pump in 0.2 mL/min rate when the decellularized f lap was embedded with agarose gel in a dish. However, this study did not provide detailed information about the decellularization methods, such as detergent concentration and duration of decellularization.…”

Section: Discussioncontrasting

confidence: 99%

“…Two previous studies, which also used the same f lap model as our study, have demonstrated that perfusion in 0.2 mL/min could reach to a perfusion pressure around 60 to 120 mm Hg. 15,16 Therefore, 0.2 mL/min was chosen to be the perfusion rate in the current study. Of course, monitoring and controlling the perfusion rate will be better for future study.…”

Section: Discussionmentioning

confidence: 99%

“…This perfusion rate was based on previous reports in the literature. 15,16 For agitation alone group, the f laps were then agitated in 1% sodium dodecyl sulfate (SDS) (Sigma) and stirred with a magnetic bead for 72 hours. Whereas for the perfusion and agitation combination groups, in addition to agitation in 1% SDS solutions, the flaps were also perfused with 1% SDS by a volumetric infusion pump (Baxter, Deerfield, Ill) for 12, 24, or 72 hours as shown in Table 1.…”

Section: Decellularizationmentioning

confidence: 99%

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Decellularization of a Fasciocutaneous Flap for Use as a Perfusable Scaffold

Hogezand

Zhao

et al. 2015

Annals of Plastic Surgery

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A combination of perfusion and agitation for 24 hours or longer effectively decellularized the fasciocutaneous portion of composite tissue flaps and removed DNA content from the flap in our rat model with well-preserved vascular structure. This combined technique was superior to agitation alone. However, closed-loop circulation could not be preserved after decellularization with perfusion and/or agitation methods.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Decellularizationmentioning

confidence: 99%

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Decellularization of a Fasciocutaneous Flap for Use as a Perfusable Scaffold

Hogezand

Zhao

et al. 2015

Annals of Plastic Surgery

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“…Therefore, perfusion through the vasculature is a particularly efficient method to deliver decellularizing agents to cells and for transport of cellular material from the tissue. It should be noted that perfusion decellularization is not necessary to produce an intact, perfusable vascular network within the ECM [98]. …”

Section: Techniques To Apply Decellularization Agentsmentioning

confidence: 99%

An overview of tissue and whole organ decellularization processes

Crapo¹,

Gilbert²,

Badylak³

2011

Biomaterials

2,927

3,125

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Biologic scaffold materials composed of extracellular matrix (ECM) are typically derived by processes that involve decellularization of tissues or organs. Preservation of the complex composition and three-dimensional ultrastructure of the ECM is highly desirable but it is recognized that all methods of decellularization result in disruption of the architecture and potential loss of surface structure and composition. Physical methods and chemical and biologic agents are used in combination to lyse cells, followed by rinsing to remove cell remnants. Effective decellularization methodology is dictated by factors such as tissue density and organization, geometric and biologic properties desired for the end product, and the targeted clinical application. Tissue decellularization with preservation of ECM integrity and bioactivity can be optimized by making educated decisions regarding the agents and techniques utilized during processing. An overview of decellularization methods, their effect upon resulting ECM structure and composition, and recently described perfusion techniques for whole organ decellularization techniques are presented herein.

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“…Thus, skin/adipose tissue flap engineering has tremendous impact in the field of reconstructive surgery. Studies have shown that a completely decellularized skin flap matrix retains its vascular structure intact [13, 14]. However, details about the characterization of the acellular skin flap matrix are lacking, and the interaction between the cells and scaffold matrix during the remodeling process has not yet been investigated in vitro or in vivo .…”

Section: Introductionmentioning

confidence: 99%

Decellularized skin/adipose tissue flap matrix for engineering vascularized composite soft tissue flaps

et al. 2016

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Using a perfusion decellularization protocol, we developed a decellularized skin/adipose tissue flap (DSAF) comprising extracellular matrix (ECM) and intact vasculature. Our DSAF had a dominant vascular pedicle, microcirculatory vascularity, and a sensory nerve network and retained three-dimensional (3D) nanofibrous structures well. DSAF, which was composed of collagen and laminin with well-preserved growth factors (e.g., vascular endothelial growth factor, basic fibroblast growth factor), was successfully repopulated with human adipose-derived stem cells (hASCs) and human umbilical vein endothelial cells (HUVECs), which integrated with DSAF and formed 3D aggregates and vessel-like structures in vitro. We used microsurgery techniques to re-anastomose the recellularized DSAF into nude rats. In vivo, the engineered flap construct underwent neovascularization and constructive remodeling, which was characterized by the predominant infiltration of M2 macrophages and significant adipose tissue formation at 3 months postoperatively. Our results indicate that DSAF co-cultured with hASCs and HUVECs is a promising platform for vascularized soft tissue flap engineering. This platform is not limited by the flap size, as the entire construct can be immediately perfused by the recellularized vascular network following simple re-integration into the host using conventional microsurgical techniques.

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Development of an Acellular Bioengineered Matrix with a Dominant Vascular Pedicle

Cited by 29 publications

References 16 publications

Decellularization of a Fasciocutaneous Flap for Use as a Perfusable Scaffold

Decellularization of a Fasciocutaneous Flap for Use as a Perfusable Scaffold

An overview of tissue and whole organ decellularization processes

Decellularized skin/adipose tissue flap matrix for engineering vascularized composite soft tissue flaps

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