Development of an amperometric biosensor based on peroxidases to quantify citrinin in rice samples

Zachetti, Vanessa Gimena Lourdes; Granero, Adrián Marcelo; Robledo, Sebastián Noel; Zón, Marı́a Alicia; Fernández, Héctor

doi:10.1016/j.bioelechem.2012.12.004

Cited by 30 publications

(16 citation statements)

References 30 publications

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“…Kafi et al 2008 ; Virel et al 2010 ; Zhong et al 2011 ; Ahammad et al 2011 ). In recent years however, a considerable number of studies also dealt with the detection of other molecules, such as glucose (Alonso-Lomillo et al 2005 ), ethanol (Azevedo et al 2005 ), DNA and RNA (Fan et al 2013 ; Tran et al 2014 ; Saikrishnan et al 2014 ), l -phenylalanine (Kubota et al 2013 ), citrinin (Zachetti et al 2013 ), pyrogallol and hydroquinone (Raghu et al 2013 ), phenols (Kafi and Chen 2009 ; Liu et al 2011 ), the milk allergen β-lactoglobulin (Ruiz-Valdepeñas Montiel et al 2015 ), rotavirus titers (Li et al 2014 ), and tumor markers (Chen et al 2009 ; Kim et al 2014b ; Patris et al 2014 ) via H 2 O 2 . Notably, studies on HRP applications of medical relevance are currently particularly prevalent.…”

Section: Impact Of Recombinant Technology On Traditional and Future Hmentioning

confidence: 99%

An updated view on horseradish peroxidases: recombinant production and biotechnological applications

Krainer

Glieder

2015

Appl Microbiol Biotechnol

191

133

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Horseradish peroxidase has been the subject of scientific research for centuries. It has been used exhaustively as reporter enzyme in diagnostics and histochemistry and still plays a major role in these applications. Numerous studies have been conducted on the role of horseradish peroxidase in the plant and its catalytic mechanism. However, little progress has been made in its recombinant production. Until now, commercial preparations of horseradish peroxidase are still isolated from plant roots. These preparations are commonly mixtures of various isoenzymes of which only a small fraction has been described so far. The composition of isoenzymes in these mixed isolates is subjected to uncontrollable environmental conditions. Nowadays, horseradish peroxidase regains interest due to its broad applicability in the fields of medicine, life sciences, and biotechnology in cancer therapy, biosensor systems, bioremediation, and biocatalysis. These medically and commercially relevant applications, the recent discovery of new natural isoenzymes with different biochemical properties, as well as the challenges in recombinant production render this enzyme particularly interesting for future biotechnological solutions. Therefore, we reviewed previous studies as well as current developments with biotechnological emphasis on new applications and the major remaining biotechnological challenge—the efficient recombinant production of horseradish peroxidase enzymes.

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Section: Impact Of Recombinant Technology On Traditional and Future Hmentioning

confidence: 99%

An updated view on horseradish peroxidases: recombinant production and biotechnological applications

Krainer

Glieder

2015

Appl Microbiol Biotechnol

191

133

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“…In recent years, there has been a tremendous increase in reports on the determination of OTA and/or citrinin immunosensors based on different platforms. Rapid screening tests such as biosensors [40,41,42,43,44,45] and enzyme-linked immunosorbent assays (ELISA) [46,47,48] are emerging. A further development is their simplification, by developing colored immuno-tests, like rapid disposable membrane-based assay tests or clean-up tandem immune assay column.…”

Section: Introductionmentioning

confidence: 99%

Impact of pH on the Stability and the Cross-Reactivity of Ochratoxin A and Citrinin

Bazin

Faucet-Marquis

Monje

et al. 2013

Toxins

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Mycotoxins are secondary metabolites produced by several fungi contaminating crops. In several countries, the maximum permitted levels of mycotoxins are found in foodstuffs and feedstuffs. The common strategy of mycotoxin analysis involves extraction, clean-up and quantification by chromatography. In this paper, we analyzed the reasons of underestimation of ochratoxin A (OTA) content in wine, and overestimation of OTA in wheat, depending on the pH of the clean-up step and the simultaneous presence of citrinin (CIT). We demonstrated that the increase of pH by adding polyethylene glycol (PEG) to wine led to an underestimation of OTA by conversion of OTA into open ring ochratoxin A OP-OA. In comparing three methods of extraction and clean-up for the determination of OTA and CIT in wheat—(i) an inter-laboratory validated method for OTA in cereals using immunoaffinity column clean-up (IAC) and extraction by acetonitrile/water; (ii) a validated method using IAC and extraction with 1% bicarbonate Na; and (iii) an in-house validated method based on acid liquid/liquid extraction—we observed an overestimation of OTA after immunoaffinity clean-up when CIT is also present in the sample, whereas an underestimation was observed when OTA was alone. Under neutral and alkaline conditions, CIT was partially recognized by OTA antibodies.

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“… Detection method Title of the published paper LD LWR Ref. Amperometric biosensor based on Peroxidases Development of an amperometric biosensor based on peroxidases to quantify citrinin in rice samples 0.25 nM 1–11.6 nM Zachetti et al (2013) Micro fluidic electrochemical immunosensor Citrinin (CIT) determination in rice samples using a micro fluidic electrochemical immunosensor 0.1 ng mL −1 0.5 and 50 ng mL −1 Arévalo et al (2011) Molecular Imprinted SPR Biosensor A molecular imprinted SPR biosensor for sensitive determination of citrinin in red yeast rice 0.0017 ng mL −1 0.005–1 ng mL −1 Atar et al (2015) FRET-based immunosensor Intensified fluorescence signal of a FRET-based immunosensor for detection of citrinin using quantum dots, MSCS and Rho123 conjugated to bovine serum albumin 0.1 pM 1–6 pM Present Study …”

Section: Resultsmentioning

confidence: 99%

Citrinin detection by intensified fluorescence signal of a FRET-based immunosensor using magnetic/silica core–shell

Sadi

Bayat

Tajik

et al. 2018

Saudi Journal of Biological Sciences

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The specific immune-reaction between the anti-citrinin antibody immobilized on the surface of magnetic/silica core-shell (MSCS) and the citrinin-Rho123-BSA conjugate brings the Rho123 fluorophore as an acceptor and the QDs as a donor in close spatial proximity and causes FRET for occurring upon photo-excitation of the QDs. The novelties of this study include: (1) immobilization of the MSCS; (2) large amount of the immobilized QDs, and (3) immobilization of a large amount of Rho123 on the BSA macromolecule. Cd/Te QDs were synthesized by the simultaneous reduction of cadmium chloride and tellurium in the presence of sodium borohydride. Magnetic nanoparticles were synthesized using FeSO and FeCl. The prepared magnetic nanoparticles shelled by silica using tetraethoxysilane in the presence of ammonia. Transmission electron microscopy (TEM) analysis was used for investigating shape and monodispersity of the nanoparticles. EDC/NHS was used as a cross linking agent for immobilization of the QDs, conjugation of citrinin to amino groups of BSA, labeling of BSA with Rho123 and also for immobilization of the amino-functionalized MSCS on the immobilized QDs. Immobilization of the anti-citrinin antibody on the surface of the amino-functionalized MSCS was performed by Schiff-base mechanism. By using these three effective strategies, sensitivity of the designed nanobiosensor was incredibly enhanced as a very low limit of detection (up to 0.1 pM). The feasibility of this technique was tested by the detection of citrinin in the spiked human serum. Results showed that there was a linear correlation between the decreased fluorescence intensity of the Rho123 and increased fluorescence intensity of the QDs with increasing concentration of citrinin in the spiked samples in the range of 1-6 pM. According to obtained results, we conclude that this highly sensitive detection scheme is a easy, quick and impressive method that can be used in optical-based nanosensors.

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Development of an amperometric biosensor based on peroxidases to quantify citrinin in rice samples

Cited by 30 publications

References 30 publications

An updated view on horseradish peroxidases: recombinant production and biotechnological applications

An updated view on horseradish peroxidases: recombinant production and biotechnological applications

Impact of pH on the Stability and the Cross-Reactivity of Ochratoxin A and Citrinin

Citrinin detection by intensified fluorescence signal of a FRET-based immunosensor using magnetic/silica core–shell

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