Development of an analytical method using microchip capillary electrophoresis for the measurement of fluorescein-labeled salivary components in response to exercise stress

Capillary electrophoresis analysis of biofluids with a focus on less commonly analyzed matrices

“…Saliva and blood samples were deproteinized by acetonitrile precipitation, then the supernatant was dried and reconstituted in water before analysis. Fluorescein-labeled amines were separated and detected in saliva using a microchip CE apparatus [88], but individual components were not identified in this analysis used for profiling pre-and post-exercise effects on saliva. Fluorescein isothiocyanate (FITC) derivatized amino acids were analyzed in saliva by CZE with a pH 9.5 borate BGE.…”

New applications of cyclodextrins in electrically driven chromatographic systems: a review

“…The results obtained by the use of quartz glass or poly-methylmethacrylate microchips were similar. The measurements proved that the concentration of some components in human saliva decrease after exercise stress (Tanaka et al, 2006).…”

Utility of salivary biomarker for stress induced by dental treatment

“…First, it is impossible to measure the salivary sIgA level at the chair-side in the clinic; however, it is possible that detection systems will be developed by another group [36]. Second, the view regarding the correlation between the stress and amount of saliva flow is still controversial.…”