Development of an Analytical Method for the Determination of Amoxicillin in Commercial Drugs and Wastewater Samples, and Assessing its Stability in Simulated Gastric Digestion

Unutkan, Tuğçe; Bakırdere, Sezgin; Keyf, Seyfullah

doi:10.1093/chromsci/bmx078

Cited by 38 publications

(18 citation statements)

References 18 publications

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“…Simulated gastric juice was prepared in a 50 mL volumetric flask by adding 0.10 g NaCl, 0.35 mL HCl (36–37%), and 0.16 g pepsin . Before topping up to the 50 mL mark with deionized water, the solution was spiked to a final concentration of 100 mg/L.…”

Section: Methodsmentioning

confidence: 99%

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A rapid and sensitive reversed phase‐HPLC method for simultaneous determination of ibuprofen and paracetamol in drug samples and their behaviors in simulated gastric conditions

Borahan

Unutkan

Şahin

et al. 2018

J of Separation Science

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Paracetamol is a widely used drug for fever and pain relief. Ibuprofen is a common nonsteroidal anti‐inflammatory drug. In this study, a sensitive and accurate reversed phase high performance liquid chromatography method was developed for the simultaneous determination of ibuprofen and paracetamol. The chromatographic separation was achieved on a Phenomenex C18 (250 mm, 4.6 mm, 5 μm) column. Fifty milli molar phosphate buffer (pH 7.5) and methanol were used as mobile phase in a gradient elution mode. The retention times of paracetamol and ibuprofen were 5.7 and 10.4 min, respectively. The linearity of the developed method was established in the range of 0.25 – 250 mg/L with a correlation coefficient of 0.9998 for both analytes. The limit of detection/quantification values were found to be 0.06/0.19 and 0.08/0.26 mg/L for ibuprofen and paracetamol, respectively. The method was successfully applied in drug samples in the form of tablets and suspensions. The calculated concentrations matched with the claimed values on their prospectuses. The drug samples were studied under simulated gastric conditions to determine the behaviors of the analytes in the human body. The obtained results showed no change in the retention time of the analyte peak shapes throughout the 210 minutes.

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Section: Methodsmentioning

confidence: 99%

“…However, HPLC takes time for stabilization and optimization of parameters such as mobile phase and flow rate. In addition to this, it requires considerable amounts of organic solvents for sample elution .…”

Section: Introductionmentioning

confidence: 99%

A rapid and sensitive reversed phase‐HPLC method for simultaneous determination of ibuprofen and paracetamol in drug samples and their behaviors in simulated gastric conditions

Borahan

Unutkan

Şahin

et al. 2018

J of Separation Science

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“…The examination was conducted in the laboratories of the Ministry of Science and Technology, Department of Environment and Water using a high-performance liquid chromate-graphy device (HPLC) model (SYKAMN) of German origin and according to the conditions mentioned in the source (P1500 pump, UV2000 detector, AS3000 automatic sampling device). (Unutkan et al, 2018) used the carrier phase consisting of (acetonitrile: methanol: phosphite bfer) according to the following ratios (10:30:60) (V/V/V), and a separation column (C18-ODS (25 CM X) was used. 4.6 mm) using an ultraviolet detector (UV -230 nm) at a flow rate (1 ml min -1 ).…”

Section: -Amoxicillinmentioning

confidence: 99%

Detection of Antibacterial Residues in Nile Tilapia Oreochromis niloticus (L.) in the Shatt Al-Arab river, Southern Iraq

Ahmed

Resen

Al-Niaeem

2022

Basrah J. Agric. Sci.

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The presence of Antibacterial in the aquatic environment is a major concern because of the effect of Antibacterial on water quality, aquatic organisms and human health. The current study aims to detect Antibacterial (Amoxicillin, Ciprofloxacin, and Levofloxacin) seasonally in water, sediments, muscles and liver of Nile tilapia fish during the period from November 2020 to August 2021 in two selected stations in Shatt Al-Arab, Basrah, Iraq. The samples were analyzed using high performance liquid chromatography (HPLC), High concentrations of the antibiotic Ciprofloxacin (CIP) were recorded in each of the water, sediment and fish muscle samples during the spring season at the second station, reaching the 24.9 mg l1-, 24.5 mg. kg1-, 7.4 mg. kg1-, respectively, while the high concentration of Amoxicillin )AMO( Antibacterial was 4.1 mg.kg-1 in liver of fish in spring season in the second station. The presence of Antibacterial in the aquatic environment in such high concentrations is a source of great concern. The study recommends conducting more studies to measure Antibacterial contamination in other areas of the Shatt al-Arab and the Iraqi marshes.

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“…A range of analytical methods has been used to detect penicillins, including mass spectrometry, chromatography, or their combination [17,18]. However, they suffer from some limitations mainly relating to sample preparation and overall complexity [19]. Hence, the need for straightforward screening measurements makes it interesting to design sensors.…”

Section: Introductionmentioning

confidence: 99%

Development of a MIP-Based QCM Sensor for Selective Detection of Penicillins in Aqueous Media

et al. 2021

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Pharmaceuticals wastes have been recognized as emerging pollutants to the environment. Among those, antibiotics in the aquatic environment are one of the major sources of concern, as chronic, low-dose exposure can lead to antibiotic resistance. Herein, we report on molecularly imprinted polymers (MIP) to recognize penicillin V potassium salt (PenV-K), penicillin G potassium salt (PenG-K), and amoxicillin sodium salt (Amo-Na), which belong to the most widespread group of antibiotics worldwide. Characterization and optimization led to two MIPs comprising methacrylic acid as the monomer and roughly 55% ethylene glycol dimethacrylate as the crosslinker. The obtained layers led to sensitive, selective, repeatable, and reusable sensor responses on quartz crystal microbalances (QCM). The LoD for PenV-K, PenG-K, and Amo-Na sensors are 0.25 mM, 0.30 mM, and 0.28 mM, respectively; imprinting factors reach at least around three. Furthermore, the sensors displayed relative selectivity factors of up to 50% among the three penicillins, which is appreciable given their structural similarity.

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Development of an Analytical Method for the Determination of Amoxicillin in Commercial Drugs and Wastewater Samples, and Assessing its Stability in Simulated Gastric Digestion

Cited by 38 publications

References 18 publications

A rapid and sensitive reversed phase‐HPLC method for simultaneous determination of ibuprofen and paracetamol in drug samples and their behaviors in simulated gastric conditions

A rapid and sensitive reversed phase‐HPLC method for simultaneous determination of ibuprofen and paracetamol in drug samples and their behaviors in simulated gastric conditions

Detection of Antibacterial Residues in Nile Tilapia Oreochromis niloticus (L.) in the Shatt Al-Arab river, Southern Iraq

Development of a MIP-Based QCM Sensor for Selective Detection of Penicillins in Aqueous Media

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