Development of an antidiabetic formulation (ADJ6) and its inhibitory activity against α-amylase and α-glucosidase

Abstract

There has recently been much advancement in the diagnosis, treatment, and research of metabolic disorders, especially diabetes. Current research around the world is focused on finding an alternative source of treatment from natural resources for diabetic management, apart from the available synthetic medicines. The present study is a preliminary study of a polyherbal formulation using edible natural resources and an assessment of its antidiabetic activity. The formulation was screened for its phytochemical constituents, total phenols, flavonoids, and vitamin C content. It was also analyzed for its inhibitory effect against the digestive enzymes α-amylase and α-glucosidase, compared with the standard drug acarbose. The formulation showed the presence of major constituents such as steroids, cardiac glycosides, phenols, flavonoids, and saponins. It also had a high level of phenols (340 ± 2.5 mg/g), flavonoids (235.4 ± 8.3 mg/g), and vitamin C (470.8 ± 16.6 mg/g), and showed a half-maximal inhibitory concentration (IC50) value of 0.41 ± 0.03 mg/mL and 0.51 ± 0.01 mg/mL for amylase and glucosidase, respectively. The results showed that ADJ6 had a significant inhibitory activity on α-amylase and α-glucosidase; however, its inhibitory activity was less than that of acarbose. The plants that are formulated in ADJ6 possess potent antidiabetic activity. Thus, we found that ADJ6 is a potent lead for effective diabetic management; however, an evaluation of the formulation must be illustrated using an in vivo model.

An update on natural compounds in the remedy of diabetes mellitus: A systematic review

“…Another polyherbal, ADJ6, a combination of six herbs, Memordica charantia, Psidium guajava, Phyllanthus emblica, Trigonella foenum-graecum, Syzygium cumini and Gymnema sylvestre, has shown a synergistic effect to inhibit α-amylase and α-glucosidase, whereas they suggested to evaluate further in diabetic animal models. 92 …”

Development of an antidiabetic polyherbal formulation (ADPHF6) and assessment of its antioxidant activity against ROS-induced damage in pUC19 and human lymphocytes – an in vitro study

“…The water-soluble proteins that exist in natural sources possessing inhibitory activity for α-amylase and α-glucosidase enzymes can be efficiently condensed to a fine powder in stable form by involving freeze-drying and aqueous extraction method [19,20]. All the individual parts are finely crushed and freeze-dried to minimize the loss of bioactive components.…”

Antioxidant Properties of ADJ6 and ITS Effect on Isolated Human Erythrocyte Ghost Membrane

“…6 The individual plants were minced using a mixer and freeze dried to prevent the loss of bioactive components. ADJ6 aqueous extract was prepared mixing them proportionally, suspending the components in water for 24 hrs and then filtered through Whatman No.1 filter paper.…”

“…The Ayurvedic methods of preparing herbal formulations are primarily aqueous in order to denaturing the vital components. 6 The aqueous extract was used for antioxidant studies and studies involving erythrocytes.…”