Development of an antigen specific colloidal gold immunochromatographic assay for detection of antibody to M. wenyonii in bovine sera

Zhao, Yang; Ren, Zhaoyu; Qian, Kang; Chen, Yingbin; Wang, Xuewei; Tang, Xinhao; Zhang, Fumei; Qin, Jianhua

doi:10.1016/j.mimet.2017.10.002

Cited by 6 publications

(4 citation statements)

References 15 publications

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“…In addition to the bla OXA-22 gene, R. mannitolilytica showed the presence of the intrinsically species-specific class D oxacillinase bla OXA-443 and bla OXA-444 genes, and the serin-hydrolase class C family beta-lactamase (GenBank Accession number WP_045219476). Biofilm production capacity was evaluated by BioTimer-resazurin assay (Canvax Biotech, Spain) as previously described 9. Both isolates produced biofilm in a dose-dependent manner with bacterial concentrations as low as 75 live cells/mL.…”

Section: Culture and Characterization Of Strainsmentioning

confidence: 99%

A case of persistent bacteraemia by Ralstonia mannitolilytica and Ralstonia pickettii in an intensive care unit

Basso¹,

Venditti²,

Raponi³

et al. 2019

IDR

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The Ralstonia spp . genus is a group of non-fermentative, Gram-negative bacteria often resistant to many antibiotics, which are emerging as opportunistic pathogens frequently associated with infections in hospital settings. We present herein a case of combined R. pickettii and R. mannitolilytica persisting and relapsing bacteraemia, possibly caused by a septic arterial thrombosis secondary to the rupture of an internal carotid artery aneurysm. Microbiology studies showed that both Ralstonia isolates produced biofilm and carried class D oxacillinase genes. When confronted with infections caused by members of the Ralstonia genus, identification to the species level is crucial for correct clinical management, as the two species show different antibiotic susceptibility patterns.

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Section: Culture and Characterization Of Strainsmentioning

confidence: 99%

A case of persistent bacteraemia by Ralstonia mannitolilytica and Ralstonia pickettii in an intensive care unit

Basso¹,

Venditti²,

Raponi³

et al. 2019

IDR

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“…Our study contains some slight differences from previously published GICG methods (Alvarez et al 2010 ; Bai et al 2022 ; Chu et al 2005 ; Costa et al 2016 ; Zhao et al 2017 ). Firstly, we improved the sensitivity of the test reaction by optimizing the reaction conditions.…”

Section: Discussionmentioning

confidence: 63%

Development and evaluation of a test strip for the rapid detection of antibody against equine infectious anemia virus

Zhang,

Guo,

Chu

et al. 2024

Appl Microbiol Biotechnol

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Equine infectious anemia (EIA) is a contagious disease of horses caused by the equine infectious anemia virus (EIAV). The clinical signs at the acute phase include intermittent high fever, thrombocytopenia, hemorrhage, edema, and anemia. The clinical signs at chronic and relapsing subclinical levels include emaciation and progressive weakness. Surviving horses become lifelong carriers because of the integration of the viral genome into that of the host, and these horses can produce and transmit the virus to other animals. This increases the difficulty of imposing practical control measures to prevent epidemics of this disease. Serological tests measuring the antibodies in equine sera are considered to be a reliable tool for the long-term monitoring of EIA. However, the standard serological tests for EIV either have low sensitivity (e.g., agar gel immunodiffusion test, AGID) or are time consuming to perform (e.g., ELISA and western blotting). The development of a rapid and simple method for detecting the disease is therefore critical to control the spread of EIA. In this study, we designed and developed a colloidal gold immunochromatographic (GICG) test strip to detect antibodies against EIAV based on the double-antigen sandwich. Both the p26 and gp45 proteins were used as the capture antigens, which may help to improve the positive detection rate of the strip. We found that the sensitivity of the test strip was 8 to 16 times higher than those of two commercially available ELISA tests and 128 to 256 times higher than AGID, but 8 to 16 times lower than that of western blotting. The strip has good specificity and stability. When serum samples from experimental horses immunized with the attenuated EIAV vaccine (n = 31) were tested, the results of the test strip showed 100% coincidence with those from NECVB-cELISA and 70.97% with AGID. When testing clinical serum samples (n = 1014), the test strip surprisingly provided greater sensitivity and a higher number of “true positive” results than other techniques. Therefore, we believe that the GICG test strip has demonstrated great potential in the field trials as a simple and effective tool for the detection of antibodies against EIAV. Key points • A colloidal gold immunochromatographic (GICG) fast test strip was developed with good specificity, sensitivity, stability, and repeatability • The test strip can be used in point-of-care testing for the primary screening of EIAV antibodies • Both the p26 and gp45 proteins were used as the capture antigens, giving a high positive detection rate in the testing of experimentally infected animal and field samples

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“…The colloidal gold Immunochromatography assay has been used for the detection of many pathogens, including bacteria, parasites, and viruses, in addition to many other hormones in the milk or sera. [117][118][119][120] The GICA was showing a promising trend in the diagnosis of some high influenza virus strains, including influenza type B and some of the highly pathogenic influenza subtypes, such as H7N7. 121,122 The GIA has many advantages as a rapid, sensitive technique that do not require sophisticated, expensive types of equipment or highly trained personnel to conduct it.…”

Section: The Gold-immunochromatography Assay (Gica)mentioning

confidence: 99%

The next‐generation coronavirus diagnostic techniques with particular emphasis on the SARS‐CoV‐2

Hemida

2021

Journal of Medical Virology

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The potential zoonotic coronaviruses (SARS-CoV, MERS-CoV, and SARS-CoV-2) are of global health concerns. Early diagnosis is the milestone in their mitigation, control, and eradication. Many diagnostic techniques are showing great success and have many advantages, such as the rapid turnover of the results, high accuracy, and high specificity and sensitivity. However, some of these techniques have several pitfalls if samples were not collected, processed, and transported in the standard ways and if these techniques were not practiced with extreme caution and precision. This may lead to false-negative/positive results. This may affect the downstream management of the affected cases. These techniques require regular finetuning, upgrading, and optimization. The continuous evolution of new strains and viruses belong to the coronaviruses is hampering the success of many classical techniques. There are urgent needs for next generations of coronaviruses diagnostic assays that overcome these pitfalls. This new generation of diagnostic tests should be able to do simultaneous, multiplex, and high-throughput detection of various coronavirus in one reaction. Furthermore, the development of novel assays and techniques that enable the in situ detection of the virus on the environmental samples, especially air, water, and surfaces, should be given considerable attention in the future. These approaches will have a substantial positive impact on the mitigation and eradication of coronaviruses, including the current SARS-CoV-2 pandemic.

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Development of an antigen specific colloidal gold immunochromatographic assay for detection of antibody to M. wenyonii in bovine sera

Cited by 6 publications

References 15 publications

<p>A case of persistent bacteraemia by <em>Ralstonia mannitolilytica</em> and <em>Ralstonia pickettii</em> in an intensive care unit</p>

<p>A case of persistent bacteraemia by <em>Ralstonia mannitolilytica</em> and <em>Ralstonia pickettii</em> in an intensive care unit</p>

Development and evaluation of a test strip for the rapid detection of antibody against equine infectious anemia virus

The next‐generation coronavirus diagnostic techniques with particular emphasis on the SARS‐CoV‐2

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