Accidents while working in the construction industry source many tangible and intangible losses. Although various measures have been taken to reduce occupational accidents in the construction sector, occupational accidents are still increasing, especially in Indonesia. Meanwhile there is some literature on the relationship between personality traits and accidents, much remains to be considered before applying the research to accident prevention. Therefore, there is a need to study in detail the correlations between the Big 5 characteristics and safety behaviors, including other variables, so that preventive action can be taken as a form of occupational injury prevention. The purpose of this study was to analyze the impact of the Big 5 personality traits, using personal values and safety culture as variables influencing safety behavior in construction workers. This study analyzes or resolves not only the direct impact of Big 5 personality traits on safety behavior of construction workers, but also the indirect impact through personal values and safety culture. Data were collected through interviews with 300 construction workers in Surabaya, Malang and Batu, East Java, Indonesia. The results showed that the Big 5 personality had a significant positive impact on workplace safety behavior with a difference of 0.299. In addition, personal values and safety culture as intervention variables may influence the impact of Big 5 personality traits on workers' safety behavior. The 0.788 and 0.545 Big 5 personalities have a significant impact on personal values and safety culture. One of the dominant indicators when measuring the Big 5 personality structure is neuroticism, with the highest stress factor of 0.928. Therefore, if management wants to improve worker safety behavior, it should conduct a workplace analysis to hire competent workers with a high degree of neuroticism