Development of an efficient micropropagation system for Dioscorea bulbifera L. and phytochemical profile of regenerated plants

Abstract

Background The present study was designed to develop an improved in vitro regeneration system for Dioscorea bulbifera using mature nodal explants. Direct organogenesis from nodal segments was achieved by culturing the nodal explants on Murashige and Skoog medium supplemented with 3.5 mgl−1 6-benzylaminopurine (BAP). Shoot multiplication was widely affected by the kind and concentration of plant growth regulators, and the sub-culturing of in vitro regenerated shootlets on fresh medium. After incubation for 4 weeks at optimum BAP concentration, cultures were transferred to secondary medium with BAP (optimized concentration) supplemented with different auxins. Results On medium with 3.5 mgl−1 6-benzylaminopurine, maximum regeneration (87 ± 1.85%) with an average shoot number of 2.0 ± 0.08, and length of 3.5 ± 0.04 cm were observed after 4 weeks of incubation. Maximum number of shoots (3.9 ± 0.21) was observed with 3.5 mgl−1 BAP in combination with 0.75 mgl−1 indole-3-acetic acid. The best root formation was observed on ½ MS medium supplemented with 0.75 mgl−1 α-naphthalene acetic acid, with 4.7 ± 0.03 roots per shoot. The well-rooted plantlets were successfully acclimatized in with 100% survival rate. The plantlets grew well with normal growth, flowering and showed, by High performance thin layer chromatography, almost same number of phytoconstituents compared with the mother plant. Conclusions This is the first study on comparative phytochemical analysis of wild growing and in vitro regenerated plants of D. bulbifera which validates the medicinal and nutritional properties of in vitro raised plants.

Effects of Low-Dose Kinetin, 2,4-D and Monochromatic Light Conditions on Flavonoid Content in Callus Culture of Dioscorea esculenta

“…In the culture study of Dioscorea esculenta L., using kinetin combined with light treatment significantly increased the percentage of culture biomass [24]. In another study, using kinetin greatly affected the growth of the tip of the propagate shoot of D. trifida L., with an optimal concentration of 0.5 mg/L [25,26]. In addition, kinetin also significantly increases the phytochemical content in callus cultures of Dioscorea bulbifera L. The results are relevant to this study, where kinetin under light conditions resulted in the growth of D. esculenta callus culture faster than 2,4-D at the same dose and conditions.…”

Efecto de sustratos orgánicos en la producción de plántulas de papa voladora (Dioscorea bulbifera L.), en Santo Domingo de los Tsáchilas

“…En la tabla 1, para la variable días a brotación de los bulbos de papa voladora, se observa que no existen diferencias significativas en los tratamientos, observándose que la aplicación de compost presenta menor tiempo a brotación con 7.50 días, seguido del bocashi con 9.20; por lo que el testigo presenta la mayor cantidad de días a brotación con 16.70. Según Hussain et al (2022), en su investigación sobre el desarrollo eficiente de la micropropagación para Dioscorea bulbifera, evidencian formación de brotes, de calidad, vigorosos y con potencialidad a los 30 días, a diferencia de esta investigación que con el tratamiento con la aplicación de compost se obtuvo formación de brotes a los 7.50 días, evidenciando la eficiencia del uso de abonos orgánicos en la producción de hortalizas.…”

Recent bioanalytical methods for the isolation of bioactive natural products from genus Codonopsis

“…26,27 An high-performance thin-layer chromatography (HPTLC) method was used for the separation of a few compounds on silica gel by automated multiple development (AMD) using solvent gradients, and later applied to check significant variation in the content of these pharmacologically active compounds in commercial samples. [28][29][30] Very recently, another rapid and sensitive HPTLC method was established for quantitative estimation of xanthones in the Radix Codonopsis. 450 enzymes, as analysed by liquid chromatography-electrospray ionisation-mass spectrometry (LC-ESI-MS).…”

“…LD 50 of 666-778 mg/kg in mice and had no effect on the central nervous system 2 (Figure 1). (30) were also obtained from the aerial parts of C. thalictrifolia. 73 Besides these, luteolin (22) and luteolin-7-O- (31) were identified from the aerial parts of C. clematidea.…”