Development of an electrochemical immunosensor to determine zearalenone in maize using carbon screen printed electrodes modified with multi-walled carbon nanotubes/polyethyleneimine dispersions

Riberi, Walter Iván; Tarditto, Lorena Viviana; Zón, Marı́a Alicia; Arévalo, Fernando Javier; Fernández, Héctor

doi:10.1016/j.snb.2017.07.113

Cited by 89 publications

(30 citation statements)

References 33 publications

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“…Such unique improvements in the analytical sensitivity of the OWLS technique compared to the corresponding ELISA method have previously been evidenced for the detection of other analytes, including a nearly three orders of magnitude enhancement for the endocrine biomarker protein vitellogenin [ 77 ] and a six orders of magnitude improvement for a herbicide active ingredient trifluralin [ 76 ]. Moreover, the current OWLS immunosensor represents substantial advancements compared to previous immunosensors for ZON, e.g., based on SPR [ 71 ], TIRE [ 73 ], and electrochemical detection with antibodies immobilised on gold nanoparticles embedded on multi-walled carbon nanotubes [ 42 ], to which the LOD of the current competitive OWLS immunosensor represents 30,000-, 5000-, and 75-fold improvements, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…These immunoassays were further amplified with fluorescent quantum dots [ 34 , 35 , 36 ], magnetic nanoparticles [ 37 ], or helical carbon nanotubes [ 38 ]. Alternatively, antibodies [ 24 , 36 , 39 , 40 , 41 , 42 , 43 , 44 ] or molecularly imprinted polymers [ 45 , 46 , 47 ] could be applied for affinity chromatography or pre-column sample purification prior to chromatographic analyses (HPLC, UPLC). Similarly, nanoparticle-assisted lateral flow immunochromatographic strips [ 48 , 49 ] were devised, occasionally with surface-enhanced Raman scattering detection [ 50 ].…”

Section: Introductionmentioning

confidence: 99%

“…Label-free biosensors on the basis of antibodies [ 57 , 58 , 59 , 60 ], aptamers [ 58 , 61 , 62 , 63 , 64 , 65 , 66 , 67 ], or molecularly imprinted polymers [ 68 , 69 , 70 , 71 ] as recognition elements have also been developed with various signal amplification and detection routes involved, and the range of sensoric detection techniques is expanding [ 72 ]. Thus, a surface plasmon resonance (SPR) biosensor has been developed for the simultaneous detection of four mycotoxins, with an LOD below 0.2 ng/mL for ZON [ 73 ], a gold nanoparticle-amplified imaging SPR (iSPR) biosensor allowed an LOD for ZON of 59.2 pg/mL in multiplex mycotoxin determination [ 74 ], a method of total internal reflection ellipsometry (TIRE) allowed detection of ZON at concentrations as low as 0.1 ng/mL [ 75 ], and electrochemical sensors resulted in LODs of 0.15–0.25 pg/mL [ 42 , 60 ]. The immunosensors developed allow rapid quantitative determination of the target compounds in plant samples and in environmental matrices, mainly in ground water.…”

Section: Introductionmentioning

confidence: 99%

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Direct and Competitive Optical Grating Immunosensors for Determination of Fusarium Mycotoxin Zearalenone

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Adányi

Szendrő

et al. 2021

Toxins

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Novel optical waveguide lightmode spectroscopy (OWLS)-based immunosensor formats were developed for label-free detection of Fusarium mycotoxin zearalenone (ZON). To achieve low limits of detection (LODs), both immobilised antibody-based (direct) and immobilised antigen-based (competitive) assay setups were applied. Immunoreagents were immobilised on epoxy-, amino-, and carboxyl-functionalised sensor surfaces, and by optimising the immobilisation methods, standard sigmoid curves were obtained in both sensor formats. An outstanding LOD of 0.002 pg/mL was obtained for ZON in the competitive immunosensor setup with a dynamic detection range between 0.01 and 1 pg/mL ZON concentrations, depending on the covalent immobilisation method applied. This corresponds to a five orders of magnitude improvement in detectability of ZON relative to the previously developed enzyme-linked immonosorbent assay (ELISA) method. The selectivity of the immunosensor for ZON was demonstrated with structural analogues (α-zearalenol, α-zearalanol, and β-zearalanol) and structurally unrelated mycotoxins. The method was found to be applicable in maize extract using acetonitrile as the organic solvent, upon a dilution rate of 1:10,000 in buffer. Thus, the OWLS immunosensor method developed appears to be suitable for the quantitative determination of ZON in aqueous medium. The new technique can widen the range of sensoric detection methods of ZON for surveys in food and environmental safety assessment.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Direct and Competitive Optical Grating Immunosensors for Determination of Fusarium Mycotoxin Zearalenone

Székács

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Szendrő

et al. 2021

Toxins

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“…Riberi et al developed CNT-based electrochemical immunosensors to quantifyzearalenone (ZEA) by using carbon screen-printed electrodes modified with gold nanoparticles and MWCNT dispersions. The developed immunosensor displayed a limit of detection (0.15 pg/mL) and sensitivity (SC50 ¼ 2 pg/mL) with an added advantage of minimum pre-treatment [115]. In another study, Aziz et al designed an amperometric immunosensor made up of Indium tin oxide (ITO) electrodes to detect the mouse IgG.…”

Section: Cnts-immunosensorsmentioning

confidence: 99%

A review on carbon nanotubes in biosensor devices and their applications in medicine

et al. 2018

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In recent years, integrating biological components in analytical instruments especially in biomedical research has become a prerequisite for early diagnosis of many diseases. It is well known that the material properties (electrical and physical) of CNTs is very sensitive to be affected by exposure to biomolecules and this led to the investigation by many researchers. Though the CNT-based biosensors has been widely used due their better performance, it still has many practical concerns in application. For the successful commercialization of the concept of CNT-based biosensors, many hurdles need to overcome. Modifications on CNT biosensors have experienced a dramatic change with outstanding developments. The present article provides an overview on the recent development in CNT biosensors and comprehensive analysis was given on various ways to improve the performance of CNT with new designs. In addition, some of the practical applications and concerns in the field are addressed. The scientific and technological challenges in the field are discussed in the conclusion.

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“…Zearalenone is a mycotoxin, which have a structure of estrogenic lactone; they have sufficient structural similarity and these synthesized by various Fusarium species-F. graminearum, F. culmorum and F. crookwellense. Zearalenone is found in cereals, mainly maize, and processed foods and these are a non-highly toxic mycotoxin [10,22]. These mycotoxins have been producing of estrogenic effects in animals and the stimulation of human breast cancer cells growth.…”

Section: Zearalenonementioning

confidence: 99%

Fusarium Mycotoxins and Metabolites that Modulate Their Production

Jiménez-García¹,

García-Mier²,

García‐Trejo³

et al. 2018

Fusarium - Plant Diseases, Pathogen Diversity, Genetic Diversity, Resistance and Molecular Markers

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The genus Fusarium is a group of fungi producing several types of toxins with toxicological effect in both humans and animals. Such fungi are commonly found in soils so it can contaminate various types of crops, preferably cereals, leading to significant economic losses. Relative humidity, storage temperature and various handling in cereales increase the possibility of contamination by Fusarium toxins. Cereals naturally have secondary metabolites that may help attenuate contamination by these toxins, but it is necessary to know strategies and mechanisms that generate inactivation mycotoxins. This chapter reviews relevant information about cereal mycotoxin contamination, as well as the production of cereal secondary metabolites as a strategy to reduce the possibility of mycotoxin contamination.

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Development of an electrochemical immunosensor to determine zearalenone in maize using carbon screen printed electrodes modified with multi-walled carbon nanotubes/polyethyleneimine dispersions

Cited by 89 publications

References 33 publications

Direct and Competitive Optical Grating Immunosensors for Determination of Fusarium Mycotoxin Zearalenone

Direct and Competitive Optical Grating Immunosensors for Determination of Fusarium Mycotoxin Zearalenone

A review on carbon nanotubes in biosensor devices and their applications in medicine

Fusarium Mycotoxins and Metabolites that Modulate Their Production

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