Development of an environmentally friendly formulation of silk fibroin combined with fatty acid from Astrocaryum murumuru Mart. effective against Aedes aegypti larvae

Marinho, Victor H.S.; Neves, Fernando B.; Jimenez, David Esteban Quintero; Oliveira, Fábio Rodrigues de; Santos, Abrahão Victor T.L.T.; Ferreira, Ricardo M. A.; Souto, Raimundo Nonato Picanço; Carvalho, J.C.T.; Yoshioka, Sérgio Akinobu; Ferreira, Irlon M.

doi:10.1016/j.jddst.2022.103626

Cited by 5 publications

(3 citation statements)

References 37 publications

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“…The nanoparticles were prepared based on the method described by Marinho et al, 2022 with slight modifications. The nanoparticles were produced by an emulsification process, using water with 6% kojic acid and 2% silk fibroin solution.…”

Section: Methodsmentioning

confidence: 99%

Polymeric nanoparticles containing kojic acid induce structural alterations and apoptosis-like death in Leishmania (Leishmania) amazonensis

Queiroz-Souza,

Galue-Parra,

Silveira Moraes

et al. 2024

Front. Pharmacol.

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Leishmaniasis encompasses a cluster of neglected tropical diseases triggered by kinetoplastid phatogens belonging to the genus Leishmania. Current therapeutic approaches are toxic, expensive, and require long-term treatment. Nanoparticles are emerging as a new alternative for the treatment of neglected tropical diseases. Silk Fibroin is a biocompatible and amphiphilic protein that can be used for formulating nanoemulsions, while kojic acid is a secondary metabolite with antileishmanial actions. Thus, this study evaluated the efficacy of a nanoemulsion, formulated with silk fibroin as the surfactant and containing kojic acid (NanoFKA), against promastigote and amastigote forms of Leishmania (Leishmania) amazonensis. The NanoFKA had an average particle size of 176 nm, Polydispersity Index (PDI) of 0.370, and a Zeta Potential of −32.3 mV. It presented inhibitory concentration (IC50) values of >56 μg/mL and >7 μg/mL for the promastigote and amastigote forms, respectively. Ultrastructural analysis, cell cycle distribution and phosphatidylserine exposure showed that NanoFKA treatment induces apoptosis-like cell death and cell cycle arrest in L. (L.) amazonensis. In addition, NanoFKA exhibited no cytotoxicity against macrophages. Given these results, NanoFKA present leishmanicidal activity against L. (L.) amazonensis.

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Section: Methodsmentioning

confidence: 99%

Polymeric nanoparticles containing kojic acid induce structural alterations and apoptosis-like death in Leishmania (Leishmania) amazonensis

Queiroz-Souza,

Galue-Parra,

Silveira Moraes

et al. 2024

Front. Pharmacol.

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“…Increased consumer demand for environmentally and socially responsible goods drives this sustainability movement forward. Sustainable formulation ideas are becoming an increasingly important focus for businesses to align themselves with the most recent green trends and address the environmental effects of their goods (Marinho et al, 2022). Conventional formulations often produce excessive waste or depend on non-renewable resources.…”

Section: Introductionmentioning

confidence: 99%

Microemulsion technology: Nanoparticle-based emulsion as a sustainable natural product formulation

Sembiring,

Arsyadi,

Riadi

2024

Pharm Educ

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Natural products refer to chemicals or compounds from living creatures occurring naturally in the environment. Natural commodities, such as spices, herbs, and medical remedies, are often used as additions. Natural products have many advantages, such as their environmentally sustainable nature, compatibility with the skin, affordability, and widespread availability. Nevertheless, developing natural products has several challenges, such as limited options for ingredients, functional limitations of natural substances, considerations about stability and preservation, consumer perceptions and expectations, and the variability in the composition of natural products. Microemulsions are prevalent emulsions that incorporate nanoparticles. A microemulsion is a homogeneous, transparent liquid consisting of oil, water, and a surfactant, often combined with a cosurfactant. The use of microemulsions has been extensively observed in many chemical and pharmacological applications owing to their unique attributes, such as a significant interfacial area, little interfacial tension, and the ability to solubilise and deliver hydrophobic substances. Therefore, the present review paper aims to comprehensively analyse microemulsion technology and its applications as an environmentally friendly formulation for natural products. Here, we propose strategies and research on microemulsions to overcome problems in natural product formulations.

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“…SF is a natural polymeric biomaterial produced by a variety of silkworm larvae, including Bomboxy mori (Ferreira et al 2017a), that meets the requirements of drug delivery systems by exhibiting advantageous properties such as low toxicity, biodegradability, biocompatibility, mechanical strength, self-assembly ability, dissolution resistance, low manufacturing cost, resistance to thermal and enzymatic degradation (Mottaghitalab et al 2015). This biomaterial has been shown to favor the biological efficacy of various substances and also to control release rates (Sarquis et al 2020;Marinho et al 2022). Due to the large number of amino acids and functional groups, fibroin can be tested in combination with a wide variety of isolated substances (Ferreira et al 2017b;Araújo et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory potential of baicalein combined with silk fibroin protein in a zebrafish model (Danio rerio)

et al. 2022

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Baicalein (BA) is a flavonoid with wideranging pharmacological activity. However, its biological evaluation is hampered by its low solubility in aqueous medium, making forms of incorporation that improve its solubility necessary. In the present study, BA was combined with a solution of silk fibroin protein (SF), a biomaterial used too as a drug carrier, to evaluate the anti-inflammatory potential of this combination, in vivo, in an experimental model, zebrafish (Danio rerio). Baicalein-silk fibroin (BASF) improved the DPPH (2,2-diphenyl-1-picrylhydrazyl-hydrate) free radical scavenging rate (95%) in comparison with BA in solution. The acute toxicity study and histopathological analysis in zebrafish showed that BASF has low cytotoxic potential, except for the maxim dose of 2000 mg/kg. The use of BA in combination with SF enhanced the anti-inflammatory effect of flavonoids by inducing inflammatory peritoneal edema through carrageenan and achieved 77.6% inhibition of abdominal edema at a dose of 75 mg/kg. The results showed that the BASF, significantly increases the bioavailability and therapeutic effect of flavonoids and several results observed in this study may help in the development of new drugs.

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Development of an environmentally friendly formulation of silk fibroin combined with fatty acid from Astrocaryum murumuru Mart. effective against Aedes aegypti larvae

Cited by 5 publications

References 37 publications

Polymeric nanoparticles containing kojic acid induce structural alterations and apoptosis-like death in Leishmania (Leishmania) amazonensis

Polymeric nanoparticles containing kojic acid induce structural alterations and apoptosis-like death in Leishmania (Leishmania) amazonensis

Microemulsion technology: Nanoparticle-based emulsion as a sustainable natural product formulation

Anti-inflammatory potential of baicalein combined with silk fibroin protein in a zebrafish model (Danio rerio)

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