Development of an experimental method to overcome the hook effect in sandwich-type lateral flow immunoassays guided by computational modelling

Sathishkumar, N.; Toley, Bhushan J.

doi:10.1016/j.snb.2020.128756

Cited by 16 publications

(19 citation statements)

References 30 publications

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“…The optimal reporter concentration ranged from 0.4 to 0.8 μM ( Fig. 4 B) and higher reporter concentrations resulted in decreasing intensity, which may be attributed to the hook effect ( Rey et al, 2017 ; Sathishkumar and Toley, 2020 ). Considering the reagent cost, we selected 0.5 μM ssDNA-BF reporter for our experiments.…”

Section: Resultsmentioning

confidence: 99%

Instrument-free, CRISPR-based diagnostics of SARS-CoV-2 using self-contained microfluidic system

Ding

Yin

et al. 2022

Biosensors and Bioelectronics

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Rapid and sensitive detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical for early diagnostics and timely medical treatment of coronavirus disease 2019 (COVID-19). However, current detection methods typically rely on expensive and bulky instrumentation. Here, we developed a simple, sensitive, instrument-free, CRISPR-based diagnostics of SARS-CoV-2 using a self-contained microfluidic system. The microfluidic chip integrates isothermal amplification, CRISPR cleavage, and lateral flow detection in a single, closed microfluidic platform, enabling contamination-free, visual detection. To simplify the operation and transportation of the device, we lyophilized the CRISPR reagents in the reaction chamber and pre-stored the liquid solutions in blisters. We employed a low-cost, portable hand warmer to incubate the microfluidic chip without the need for electricity. The self-contained microfluidic system can detect down to 100 copies of SARS-CoV-2 RNA. Further, we clinically validated our method by detecting 24 COVID-19 clinical nasopharyngeal swab samples, achieving excellent sensitivity (94.1%), specificity (100%), and accuracy (95.8%). This simple, sensitive, and affordable microfluidic system represents a promising tool for point-of-care diagnostics of COVID-19 and other infectious diseases.

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Section: Resultsmentioning

confidence: 99%

Instrument-free, CRISPR-based diagnostics of SARS-CoV-2 using self-contained microfluidic system

Ding

Yin

et al. 2022

Biosensors and Bioelectronics

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“…4a). 162,[187][188][189][190][191] When the concentration of analytes is high enough that there exist numerous unlabeled analytes in the assay, unlabeled analytes bind to the capture antibody at the test line, resulting in fewer labeled analytes captured at the test line. Several attempts have been made to alleviate the hook effect, including adding a third line that only binds conjugates 161 (Fig.…”

Section: Paper-based Diagnostics To Detect Copd-related Biomarkers In...mentioning

confidence: 99%

“…Other researchers also tried to provide theoretical explanations of the "hook" effect in LFAs due to high analyte concentrations. 188,190 Furthermore, LFAs are also limited by a mass transfer of analytes and signal particles (which is labeled antibody in sandwich LFA). 146 Diffusivity of analyte and signal particles is affected by their particle sizes and the viscosities of the media.…”

Section: Paper-based Diagnostics To Detect Copd-related Biomarkers In...mentioning

confidence: 99%

Point-of-care COPD diagnostics: biomarkers, sampling, paper-based analytical devices, and perspectives

Dong

Yang

et al. 2022

Analyst

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“…The general principle of the assay for HbA 1c is depicted in Figure 3 . Various analytes including human chorionic gonadotropin (pregnancy test) and various antibodies and antigen markers can be measured by the lateral flow immunochromatographic assay and pregnancy tests can be mentioned as a common example of the actual use of these tests [ 67 , 68 , 69 ]. On the other hand, the lateral flow immunochromatographic assay provides a semiquantitative signal only and it is not fully applicable for the quantification of a marker, though there have been promising experiments aiming to make the assay suitable for the determination of exact concentrations [ 70 ].…”

Section: Biosensors and Bioassays Measuring Hba 1cmentioning

confidence: 99%

Glycated Hemoglobin and Methods for Its Point of Care Testing

Pohanka

2021

Biosensors

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Glycated hemoglobin (HbA1c) is a product of the spontaneous reaction between hemoglobin and elevated glucose levels in the blood. It is included among the so-called advanced glycation end products, of which is the most important for the clinical diagnosis of diabetes mellitus, and it can serve as an alternative to glycemia measurement. Compared to the diagnosis of diabetes mellitus by glycemia, the HbA1c level is less influenced by a short-term problem with diabetes compensation. Mass spectroscopy and chromatographic techniques are among the standard methods of HbA1c level measurement. Compared to glycemia measurement, there is lack of simple methods for diabetes mellitus diagnosis by means of the HbA1c assay using a point-of-care test. This review article is focused on the surveying of facts about HbA1c and its importance in diabetes mellitus diagnosis, and surveying standard methods and new methods suitable for the HbA1c assay under point-of-care conditions. Various bioassays and biosensors are mentioned and their specifications are discussed.

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Development of an experimental method to overcome the hook effect in sandwich-type lateral flow immunoassays guided by computational modelling

Cited by 16 publications

References 30 publications

Instrument-free, CRISPR-based diagnostics of SARS-CoV-2 using self-contained microfluidic system

Instrument-free, CRISPR-based diagnostics of SARS-CoV-2 using self-contained microfluidic system

Point-of-care COPD diagnostics: biomarkers, sampling, paper-based analytical devices, and perspectives

Glycated Hemoglobin and Methods for Its Point of Care Testing

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