Development of an exposure indicator to hexavalent chromium: An in vitro evaluation with human blood

Intra-erythrocyte chromium as an indicator of exposure to hexavalent chromium: An in vivo evaluation in intravenous administered rat

“…A previous in vitro study conducted on human blood samples confirmed that CrIE may be a good indicator of the internal dose resulting from exposure to Cr(VI) (Devoy et al, 2017). A linear relationship between CrIE and the amount of Cr(VI) added to blood was demonstrated, as was the robustness of CrIE with respect to several parameters (blood donors, blood reducing capacities, presence of Cr(III) in the medium).…”

“…To reduce sample viscosity and dispense the sample more evenly in the graphite tube, aliquots of the RBC, plasma and urine samples were further diluted by a factor of 1:5 by adding 0.2% v/v HNO3 and 1.0% v/v Triton X-100. A 30 µL volume of diluted sample was introduced to a graphite tube and atomized according to a set temperature program (Devoy et al, 2016). Peak areas in absorbance were used for quantitative analysis.…”

“…These doses do not trigger clinical signs of toxicity (Fairhurst and Minty, 1989). In addition, the amounts injected into the blood were such that they did not exceed the reduction capacity and they had at worst a very limited impact on the hematological parameters, the viability of RBC being unaffected (Devoy et al, 2016;Ray, 2016).…”