Development of an HPLC‐DAD method for the determination of five alkaloids in <i>Stephania yunnanensis</i> Lo and in rat plasma after oral dose of <i>Stephania yunnanensis</i> Lo extracts

Xin, Aiyi; Zhang, Yaming; Zhang, Yanxia; Di, Duolong; Liu, Junxi

doi:10.1002/bmc.4292

Cited by 12 publications

(3 citation statements)

References 29 publications

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“…Palmatine is a natural isoquinoline alkaloid found in traditional Chinese medicines, such as Tinospora cordifolia, Corydalis yanhusuo , Phellodendron amurense, Tinospora sagittate, and Stephania yunnanensis [ 28 – 30 , 41 , 42 ]. Previous studies have revealed that palmatine has several therapeutic properties including neuroprotective, anti-cancer, antibacterial, and anti-inflammatory activities [ 43 – 46 ].…”

Section: Discussionmentioning

confidence: 99%

Anti-cancer effect of palmatine through inhibition of the PI3K/AKT pathway in canine mammary gland tumor CMT-U27 cells

Yoo,

Choi,

Jang

et al. 2023

BMC Vet Res

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Canine mammary gland tumors (CMTs) are the most common and lethal cancers in female dogs. Dysregulated phosphoinositide 3-kinases (PI3K)/AKT pathway reportedly was involved in the growth and metastasis of CMTs. However, there are few studies on therapeutic strategies for targeting the PI3K pathway in CMTs. In this study, we aimed to determine whether palmatine, a natural isoquinoline alkaloid with anti-cancer properties, could inhibit the growth of CMTs and whether the inhibitory effect was mediated through the PI3K/AKT pathway. Our in vitro experiments on CMT-U27, a CMT cell line, showed that palmatine reduced cell proliferation and induced cell death. Western blotting results revealed that palmatine decreased the protein expression of PI3K, PTEN, AKT, and mechanistic target of rapamycin in the PI3K/AKT pathway, which was supported by the results of immunocytochemistry. Additionally, palmatine suppressed the migration and tube formation of canine aortic endothelial cells as well as the migration of CMT U27 cells. Our in vivo results showed that palmatine inhibited tumor growth in a CMT-U27 mouse xenograft model. We observed a decreased expression of proteins in the PI3K/AKT pathway in tumor tissues, similar to the in vitro results. Furthermore, palmatine significantly disrupted the tumor vasculature and inhibited metastasis to adjacent lymph nodes. In conclusion, our findings demonstrate that palmatine exerts anti-cancer effects against CMTs by inhibiting PI3K/AKT signaling pathway, suggesting that palmatine has potential as a canine-specific PI3K inhibitor for the treatment of CMTs.

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Section: Discussionmentioning

confidence: 99%

Anti-cancer effect of palmatine through inhibition of the PI3K/AKT pathway in canine mammary gland tumor CMT-U27 cells

Yoo,

Choi,

Jang

et al. 2023

BMC Vet Res

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“…Gagnep [ 115 ], Phellodendron amurense Rupr. [ 116 ] and Stephania yunnanensis [ 117 ]. Palmatine has shown neuroprotective activity mainly by inhibiting the activity of AChE, BChE, and neuraminidase-1 [ 118 ].…”

Section: Role Of Alkaloid Compounds In Alzheimer’s Diseasementioning

confidence: 99%

Study of Nutraceuticals and Phytochemicals for the Management of Alzheimer's Disease: A Review

Ahmad

Khalid

Kamal

et al. 2021

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Background: Alzheimer's disease (AD) affecting several people worldwide and has devastating impacts in society with a limited number of approaches for its pharmacological treatment. The main causes of AD are not clear. However, the formation of senile plaques, neurofibrillary tangles, hyper-phosphorylation of tau protein, and disruption of redox homeostasis is the reason for AD. These causes have a positive correlation with oxidative stress, producing the reactive ions which are responsible for altering the physiological condition of the body. Conclusion: Ongoing research recommended the use of phytochemicals as acetylcholinesterase inhibitors to hinder AD onset and progression. The natural compound structures, including lignans, flavonoids, tannins, polyphenols, triterpenes, sterols, and alkaloids have anti-inflammatory, antioxidant, and anti-amyloidogenic properties. The purpose of this article is to provide a brief introduction to AD along with the use of natural compounds as new therapeutic approaches for its management.

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“…Stephania yunnanensis 2018 HPLC-DAD method for the determination of five alkaloids (protoberberine, morphine, aporphine and protaporphine alkaloids) in Stephania yunnanensis Lo [ 862 ].…”

Section: Routine and Improved Analyses Of Abused Substancesmentioning

confidence: 99%

Interpol review of controlled substances 2016–2019

Jones

Comparin

2020

Forensic Science International: Synergy

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Development of an HPLC‐DAD method for the determination of five alkaloids in Stephania yunnanensis Lo and in rat plasma after oral dose of Stephania yunnanensis Lo extracts

Cited by 12 publications

References 29 publications

Anti-cancer effect of palmatine through inhibition of the PI3K/AKT pathway in canine mammary gland tumor CMT-U27 cells

Anti-cancer effect of palmatine through inhibition of the PI3K/AKT pathway in canine mammary gland tumor CMT-U27 cells

Study of Nutraceuticals and Phytochemicals for the Management of Alzheimer's Disease: A Review

Interpol review of controlled substances 2016–2019

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