Development of an HPTLC-based dynamic reference standard for the analysis of complex natural products using Jarrah honey as test sample

Abstract

In this paper, we describe a novel approach to the development of a reference standard for the quality control of complex natural products, which will assist in the assessment of their authenticity and purity. The proposed method provides a template for the selection of samples, which can be pooled to obtain a reference standard. A shortfall of such an approach is, however, that the pooled sample is static in nature and therefore unable to capture difference in processing conditions or natural variations triggered by geographical or climatic impacts over time. To address this, the paper also outlines the development of a dynamic reference standard, which allows for ongoing adjustments to future variations. The method employs High-Performance Thin Layer Chromatography (HPTLC) derived extract profiles processed by multivariate analysis. The development of the dynamic reference standard is illustrated using honey, a complex natural matrix, as an example.

Bioprospecting of fungal endophytes from Oroxylum indicum (L.) Kurz with antioxidant and cytotoxic activity

“…HPTLC of the EA extract of fungal endophytes was performed using CAMAG HPTLC instrument (ANCHROM, Switzerland), comprises of a Linomat-4 autosampler, CAMAG TLC scanner-4 and visualizer with minor modification [ 27 ]. HPTLC finger printing was performed on 20 × 10 cm pre-coated silica gel 60 F 254 TLC plate (Merck, Germany).…”

An investigation of the suitability of melissopalynology to authenticate Jarrah honey

“…1 c and Rf 0.68, Fig. 3 c) can be observed at white light after derivatisation, which is considered a marker band of Banksia sessilis nectar ( Islam et al, 2021 ). This bright blue band persists after filtration (Rf 0.46, Fig.…”

The Development and Application of a HPTLC-Derived Database for the Identification of Phenolics in Honey

“…MPB was selected as the mobile phase because prior studies of honey using HPTLC analysis employed this mobile phase, allowing for cross-references to previous work. MPA, with slightly higher polarity, was chosen to ensure that more polar phenolics were also adequately separated and detected [ 36 , 37 , 38 ].…”

“…Polyethylene glycol (PEG-400) reagent was prepared by mixing 5 g of polyethylene glycol in ethanol and then the volume of the solution was made up to 100 mL (5% m/v) [ 63 ]. Vanillin sulfuric acid (VSA)-derivatising reagent was prepared by adding 2 mL of sulfuric acid to 100 mL 1% vanillin solution (1 g/100 mL in ethanol) [ 36 , 52 , 64 ]. All derivatising reagents were stored at 0 °C when not in use.…”