Development of an immunoassay for differentiating human immunodeficiency virus infections — from vaccine-induced immune response in Tiantan vaccine trials in China

Yuan, Zihao; Chen, Weixian; Zhang, Juan; Zhang, Jun; Xiang, Tingxiu; Hu, Jieli; Wu, Zhiyong; Du, Xi; Huang, Aiping; Zheng, Jie

doi:10.1016/j.clinbiochem.2012.05.013

Cited by 2 publications

(1 citation statement)

References 21 publications

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“…6b). The resultant areas were linearly proportional to the volume of the protein solution, with an 2 observed for a 5 μL protein volume. This was simply contributed by an increase in protein volume, which caused the solution to spread easily and made a bigger protein dot on the membrane.…”

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confidence: 99%

Impact of membrane pore structure on protein detection sensitivity of affinity-based immunoassay

Ahmad

Ideris

Ooi

et al. 2016

Polish Journal of Chemical Technology

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Understanding a membrane's morphology is important for controlling its fi nal performance during protein immobilization. Porous, symmetric membranes were prepared from a polyvinylidene fl uoride/N-methyl-2-pyrrolidinone solution by phase inversion process, to obtain membrane with various microsized pores. The concentration and surface area of aprotein dotted on the membrane surface were measured by staining with Ponceau S dye. The dotted protein was further scanned and analysed to perform quantitative measurements for relative comparison. The intensity of the red protein spot and its surface area varied depending on the membrane pore size, demonstrating the dependence of protein immobilization on this factor. The membrane with the smallest pore size (M3) showed the highest protein spot intensity and surface area when examined at different protein concentrations. An increase in the applied protein volume showed a linearity proportional trend to the total surface area, and an uneven round dot shape was observed at a large applied volume of protein solution.

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mentioning

confidence: 99%

Impact of membrane pore structure on protein detection sensitivity of affinity-based immunoassay

Ahmad

Ideris

Ooi

et al. 2016

Polish Journal of Chemical Technology

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Progresses in DNA-Based Heterologous Prime-Boost Immunization Strategies

Jackson

Boyle

Ranasinghe

2014

Methods in Molecular Biology

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Although recombinant DNA and recombinant viral vectors expressing HIV antigens have yielded positive outcomes in animal models, these vaccines have not been effectively translated to humans. Despite this, there is still a high level of optimism that poxviral-based vaccine strategies could offer the best hope for developing an effective vaccine against not only HIV-1 but also other chronic diseases where good-quality T and B cell immunity is needed for protection. In this chapter we discuss step by step (1) how recombinant poxviral vectors co-expressing HIV antigens and promising mucosal/systemic adjuvants (e.g., IL-13Rα2) are constructed, (2) how these vectors can be used in alternative heterologous prime-boost immunization strategies, (3) how systemic and mucosal samples are prepared for analysis, followed by (4) two immunological assays: multicolor intracellular cytokine staining and tetramer/homing maker analysis that are used to evaluate effective systemic and mucosal T cell immunity.

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Development of an immunoassay for differentiating human immunodeficiency virus infections — from vaccine-induced immune response in Tiantan vaccine trials in China

Cited by 2 publications

References 21 publications

Impact of membrane pore structure on protein detection sensitivity of affinity-based immunoassay

Impact of membrane pore structure on protein detection sensitivity of affinity-based immunoassay

Progresses in DNA-Based Heterologous Prime-Boost Immunization Strategies

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