Development of an improved medium for germination of Cajanus cajan (L.) Millsp. pollen in vitro

Jayaprakash, P.; Sarla, N.

doi:10.1093/jexbot/52.357.851

Cited by 35 publications

(27 citation statements)

References 6 publications

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“…An alternative medium with 10% PEG (polyethylene glycol 4000) was modified from [53], [54] and contained, 0.01% H 3 BO 3 , 3 mM Ca(NO 3 ) 2 , 1 mM MgSO 4 , 1 mM KNO 3 , 10% (w/v) PEG, 10% (w/v) sucrose, pH 7.5 with KOH. To make solid medium with PEG, the liquid medium minus PEG was first solidified with 1% low-melting agarose, and then equilibrated with liquid medium including 10% PEG.…”

Section: Methodsmentioning

confidence: 99%

Cyclic Nucleotide Gated Channels 7 and 8 Are Essential for Male Reproductive Fertility

et al. 2013

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The Arabidopsis thaliana genome contains 20 CNGCs, which are proposed to encode cyclic nucleotide gated, non-selective, Ca2+-permeable ion channels. CNGC7 and CNGC8 are the two most similar with 74% protein sequence identity, and both genes are preferentially expressed in pollen. Two independent loss-of-function T-DNA insertions were identified for both genes and used to generate plant lines in which only one of the two alleles was segregating (e.g., cngc7-1+/−/cngc8-2−/− and cngc7-3−/−/cngc8-1+/−). While normal pollen transmission was observed for single gene mutations, pollen harboring mutations in both cngc7 and 8 were found to be male sterile (transmission efficiency reduced by more than 3000-fold). Pollen grains harboring T-DNA disruptions of both cngc7 and 8 displayed a high frequency of bursting when germinated in vitro. The male sterile defect could be rescued through pollen expression of a CNGC7 or 8 transgene including a CNGC7 with an N-terminal GFP-tag. However, rescue efficiencies were reduced ∼10-fold when the CNGC7 or 8 included an F to W substitution (F589W and F624W, respectively) at the junction between the putative cyclic nucleotide binding-site and the calmodulin binding-site, identifying this junction as important for proper functioning of a plant CNGC. Using confocal microscopy, GFP-CNGC7 was found to preferentially localize to the plasma membrane at the flanks of the growing tip. Together these results indicate that CNGC7 and 8 are at least partially redundant and provide an essential function at the initiation of pollen tube tip growth.

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Section: Methodsmentioning

confidence: 99%

Cyclic Nucleotide Gated Channels 7 and 8 Are Essential for Male Reproductive Fertility

et al. 2013

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“…Pollen of other tropical legumes such as pigeonpea ( Cajanus cajan L.; Jayaprakash and Sarla 2001) germinates at room temperature (20 ± 1°C). Interestingly, Phaseolus , also a warm season legume, requires low temperatures for in vitro pollen germination.…”

Section: Discussionmentioning

confidence: 99%

Manipulation of in vivo pollination techniques to improve the fertilization efficiency of interspecies crosses in the genus Phaseolus

2007

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Phaseolus angustissimus A. Gray contains genes for traits of interest for dry bean (P. vulgaris) breeders. F 1 hybrids can be produced but introgression through backcrossing has been a problem. One of the main impediments is the time required between pollination and fertilization when F 1 hybrids of P. vulgaris/P. angustissimus are backcrossed with P. vulgaris. In an attempt to reduce this time, the effect of alternative pollination techniques was studied. The rate and the time of fertilization were ascertained using three different pollen types (pollen germinated in vitro, fresh pollen (FP) mixed in pollengerminating media and FP), and two methods of pollination (cut-style and stigmatic pollinations). An optimal in vitro pollen germination medium for Phaseolus pollen was developed. Low temperatures (6-9°C) were demonstrated to be necessary for Phaseolus pollen germination. Pollination using a cut-style technique coupled with pregerminated pollen reduced the time for fertilization in the backcrosses of interspecies hybrids by approximately 28 h. This technique improved the success rate of fertilization in wide crosses of Phaseolus designed to introgress genes from wild relatives.

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“…It was composed of 100mg 1-1 boric acid, 200mg 1-1 magnesium sulfate, 100mg 1-1potassium nitrate, 300-mg 1-1 calcium nitrate, 1% agar and sugars (Jayaprakash, & Sarla, 2000). Five sugars were utilized, namely; fructose, glucose, lactose, maltose and sucrose.…”

Section: Preparation Of Pollen Germination Mediummentioning

confidence: 99%

Varying Sugars and Sugar Concentrations Influence In Vitro Pollen Germination and Pollen Tube Growth of Cassia alata L.

Lagera¹,

Balinado²,

Baldomero³

et al. 2017

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Development of an improved medium for germination of Cajanus cajan (L.) Millsp. pollen in vitro

Cited by 35 publications

References 6 publications

Cyclic Nucleotide Gated Channels 7 and 8 Are Essential for Male Reproductive Fertility

Cyclic Nucleotide Gated Channels 7 and 8 Are Essential for Male Reproductive Fertility

Manipulation of in vivo pollination techniques to improve the fertilization efficiency of interspecies crosses in the genus Phaseolus

Varying Sugars and Sugar Concentrations Influence In Vitro Pollen Germination and Pollen Tube Growth of Cassia alata L.

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