Development of an Indirect Competitive ELISA for the Detection of Furazolidone Marker Residue in Animal Edible Tissues

Chang, Chao; Peng, Da-peng; Jiang, Wu; Wang, Yulian; Zhang, Yuan

doi:10.1021/jf0726684

Cited by 41 publications

(48 citation statements)

References 17 publications

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“…A detection capability of 0.3 µg kg -1 was achieved and a high correlation with LC-MS/MS was found Franek et al, 2006). Recently, Chang et al (2008) established an ELISA method for AOZ using derivatising agent o-NBA, solid phase extraction and a standard curve in PBS buffer. The resultant LOD was below 0.3 µg/kg for fish, swine and chicken tissue.…”

Section: Elisa Methods For Nitrofuran Determinationmentioning

confidence: 95%

“…As shown in Table 4, various sample preparation methods prior to analysis have been reported for a large variety of matrices such as in animal feeds (Barbosa et al, 2007), animal tissues (Verdon et al, 2007;Rodziewicz and Zawadzka, 2007;Chang et al, 2008;Cooper et al, 2008), chicken eggs Vass et al, 2008b), aquaculture products such as shrimp (Chu and Lopez, 2005) and prawn (Cooper et al, 2004b), water (Lui et al, 2007), and milk (Rodziewicz, 2008). Methods for the determination of semicarbazide presence or contamination not related to nitrofuran abuse in matrices such as carrageenan and algae (Hoenicke et al, 2004), shrimp (Bock et al, 2007b), crayfish (Saari and Peltonen, 2004) flour (Becalski et al, 2004), salt (Pereira et al, 2004) and baby food (De Souza et al, 2005) have also been established.…”

Section: Sample Preparation Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Nitrofuran antibiotics: a review on the application, prohibition and residual analysis

Vass¹,

Hruška²,

Fránek³

2008

Vet. Med.

238

130

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Nitrofuran antibiotics, employed for the treatment of bacterial diseases in livestock production, were banned from use in the European Union (EU) in 1995 due to concerns about the carcinogenicity of their residues in edible tissue. This review provides an overview of nitrofuran toxicity, metabolism, and also specific aspects of legislation surrounding their prohibition. Special attention is devoted to semicarbazide -a nitrofuran metabolite and food contaminant. Analytical procedures for nitrofuran analysis in various matrices and validation requirements for screening and confirmation methods with respect to EU regulations are also reviewed.Keywords: nitrofurans; tissue bound metabolites; semicarbazide; bioavailability; mutagenicity; legislation; sample preparation; validation; detection methods List of abbreviations AHD = 1-aminohydantoin; AOZ = 3-amino-2-oxazolidinone; AMOZ = 3-amino-5-morpholino-methyl-1,3-oxazolidinone; CC α = decision limit; CC β = detection capability; EC = European Commission; EFSA = European Food Safety Authority; ELISA = enzyme linked immuno-adsorbent assay; ESI = electro-spray ionisation; EU = European Union; FTD = furaltadone; FZD = furazolidone; HPLC = high performance liquid chromatography; IC = inhibition concentration; LC = liquid chromatography; LOD = limit of detection; MS = mass spectrometry; NFT = nitrofurantoin; NFZ = nitrofurazone; NP = nitrophenyl; NPAHD = 3-(2-nitrobenzylidenamino)-2,4-imidazolidinedione); NPAMOZ = 5-(morpholinomethyl)-3-(2-nitrobenzylidenamino)-2-oxazolidinone); NPAOZ = [3-(2-nitrobenzylidenamino)-2-oxazolidinone]; NPSEM = 3[(2-nitrophenyl)methylene]-hydrazinecarboxamide; o-NBA = ortho-nitrobenzaldehyde; RASFF = Rapid Alert System for Food and Feed; SE = solvent extraction; SEM = semicarbazide; SPE = solid phase extraction; UV = ultraviolet

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Section: Elisa Methods For Nitrofuran Determinationmentioning

confidence: 95%

Section: Sample Preparation Methodsmentioning

confidence: 99%

Nitrofuran antibiotics: a review on the application, prohibition and residual analysis

Vass¹,

Hruška²,

Fránek³

2008

Vet. Med.

238

130

View full text Add to dashboard Cite

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“…For fish, a number of NPAOZ ELISAs have been developed with LOD/LOQ values of 0.1/0.3 µg/kg (Cheng et al, 2009;Tsai et al, 2009;Liu et al, 2010b). For pork, chicken and beef muscle and liver samples, ELISA tests for NPAOZ were developed with a CCβ value of 0.4 µg/kg (Diblikova et al, 2005), with LOD values of 0.3-0.4 µg/kg (Chang et al, 2008) and with an LOD value of 1.0 µg/kg (Nesterenko et al, 2012).…”

Section: Screening Methodsmentioning

confidence: 99%

Scientific Opinion on nitrofurans and their metabolites in food

Panel¹

2015

EFS2

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Nitrofurans are antimicrobial agents not authorised for use in food-producing animals in the European Union. Nitrofurans are rapidly metabolised, occurring in animal tissues as protein-bound metabolites. The European Commission requested EFSA to provide a scientific opinion on the risks to human health related to the presence of nitrofurans in food and whether a reference point for action (RPA) of 1.0 µg/kg for the marker metabolites is adequate to protect public health. Data on occurrence of nitrofuran marker metabolites in food were extracted from the national residue monitoring plan results and from the Rapid Alert System for Food and Feed (RASFF). The CONTAM Panel concluded that these data were too limited to carry out a reliable human dietary exposure assessment. Instead, human dietary exposure was calculated for a scenario in which a single nitrofuran marker metabolite is present at 1.0 µg/kg in foods of animal origin, excluding milk and dairy products. The mean chronic dietary exposure for this worst-case scenario would range from 3.3 to 8.0 and 1.9 to 4.3 ng/kg b.w. per day for toddlers and adults, respectively. Nitrofurans and their marker metabolites, generally, are genotoxic and carcinogenic and, also, have non-neoplastic effects in animals. Margins of exposure (MOEs) were calculated at 2.0 × 10 5 or greater for carcinogenicity and at 2.5 × 10 3 or greater for non-neoplastic effects. The CONTAM Panel concluded that it is unlikely that exposure to food contaminated with nitrofuran marker metabolites at or below 1.0 µg/kg is a health concern. A scenario in which foods are considered to be contaminated with semicarbazide, from use of carrageenan as a food additive, at 1 µg/kg was used to assess whether it is appropriate to apply the RPA to foods of non-animal origin; MOEs of greater than 10 4 calculated for nonneoplastic effects do not indicate a health concern.© European Food Safety Authority, 2015 However, the opinion also identified certain categories of non-allowed pharmacologically active substances that are considered to be outside the scope of the procedure, including substances that are high potency carcinogens, such as nitrofurans. As nitrofurans are excluded from that opinion, and taking into account that the presence of SEM in food may be from sources other than use of nitrofurazone, the European Commission (EC) requested the European Food Safety Authority (EFSA) for a scientific opinion on the risks to human health related to the presence of nitrofurans and their metabolites in food. The opinion should include (a) an evaluation of the toxicity of nitrofurans and their metabolites for humans, considering all relevant toxicological endpoints and identification of the toxicological relevance of nitrofurans and their metabolites present in food, and (b) an exposure assessment of the EU population to nitrofurans and their metabolites from food, including the consumption patterns of specific (vulnerable) groups of the population. In addition, the opinion should assess the appropriateness of...

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“…Comparison with those instrumental methods, ELISA is a low cost and sensitive method capable of screening large amount of samples in a single test. Many papers involving ELISA method have been reported for detection of the metabolites of furazolidone, nitrofurantoin and nitrofurazone (Chang, Peng, Wu, Wang, & Yuan, 2008;Liu, Zhao, Zhang, Lu, Liu, & Xi, 2007;Vass, Diblikova, & Franek, 2008). However, there has been only one paper reporting ELISA method for the detection of AMOZ in shrimp (Pimpitak, Putong, Komolpis, Petsom, & Palaga, 2009).…”

Section: Introductionmentioning

confidence: 99%

Direct determination of furaltadone metabolite, 3-amino-5-morpholinomethyl-2-oxazolidinone, in meats by a simple immunoassay

Yan

Zhang

et al. 2012

Food and Agricultural Immunology

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Furaltadone is a banned drug for use in food producing animals and the marker residue of furaltadone in edible tissues is its metabolite, 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ). In this study, a novel polyclonal antibody of furaltadone was produced by using of the conjugate of furaltadone-bovine serum albumin as immunogen. The obtained antibody showed good specificity and sensitivity toward AMOZ with an IC 50 value of 2.3 ng/mL. Then, an indirect competitive immunoassay (ELISA) with heterologous coating antigen was developed to directly determine AMOZ in animal meat with a simple sample preparation. The limit of detection for AMOZ in meat was 0.4 ng/g. At three fortification levels, the intra-and inter-assay recoveries from blank samples were in a range of 85.0%Á103%. The ELISA method was further confirmed with a HPLC method in which AMOZ was determined after derivatization with 2-naphthaldehyde. The two methods showed good agreement from the analysis of incurred pork samples.

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Development of an Indirect Competitive ELISA for the Detection of Furazolidone Marker Residue in Animal Edible Tissues

Cited by 41 publications

References 17 publications

Nitrofuran antibiotics: a review on the application, prohibition and residual analysis

Nitrofuran antibiotics: a review on the application, prohibition and residual analysis

Scientific Opinion on nitrofurans and their metabolites in food

Direct determination of furaltadone metabolite, 3-amino-5-morpholinomethyl-2-oxazolidinone, in meats by a simple immunoassay

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