Development of an LC-MS/MS method to simultaneously quantify therapeutic mAbs and estimate hematocrit values in dried blood spot samples

Chiu, Huai-Hsuan; Tsai, Yun-Jung; Lo, Chiao; Liao, Hsiao Wei; Lin, Ching‐Hung; Tang, Sung‐Chun; Kuo, Ching‐Hua

doi:10.1016/j.aca.2021.339231

Cited by 8 publications

(11 citation statements)

References 33 publications

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“…The accuracy over all Hct levels was between 98% and 105%, which was within acceptable limits. Other recent studies have also determined that the effect of Hct on DBS sampling is within acceptable limits for a variety of analytes; 8-isoPGF 2α , 8-isoPGE 2 and PGE 2 [Hct 30-60%], 22 tacrolimus [Hct 18-55%], 63,64 creatinine [Hct 18-55%], 63 31 The study exhibited a positive bias exceeding 50% for Hct lower than 25% and a negative bias exceeding 30% for Hct higher than 55%. The bias was attributed to the large size of therapeutic mAbs, which prevented them from passing through the cell membranes of erythrocytes, resulting in their predominant distribution in plasma and an obvious Hct effect.…”

Section: Sub-classificationmentioning

confidence: 85%

“…However, research by Chiu et al. on the influence of Hct ranging from 15% to 65% on the quantification accuracy of four monoclonal antibodies (mAb) demonstrated a need for Hct‐based concentration correction to obtain accurate quantification results 31 . The study exhibited a positive bias exceeding 50% for Hct lower than 25% and a negative bias exceeding 30% for Hct higher than 55%.…”

Section: Microsampling Technologies and Innovationsmentioning

confidence: 97%

“…Microsampling has also been applied for the determination of tetrahydrocannabinol, cannabidiol and their respective metabolites in light cannabis smokers and patients with intractable epilepsy 130,131 . Additionally, an assay was developed to simultaneously quantify four mAbs and estimate Hct using haemoglobin peptides in DBS 31 . The large size of mAbs restricts their movement through the cell membrane of erythrocytes causing an observable Hct effect; thus, this assay greatly benefits DBS‐based mAb studies.…”

Section: Applicationsmentioning

confidence: 99%

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Blood microsampling technologies: Innovations and applications in 2022

Thangavelu

Wouters

Kindt

et al. 2023

Analytical Science Advances

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With the development of highly sensitive bioanalytical techniques, the volume of samples necessary for accurate analysis has reduced. Microsampling, the process of obtaining small amounts of blood, has thus gained popularity as it offers minimal‐invasiveness, reduced logistical costs and biohazard risks while simultaneously showing increased sample stability and a potential for the decentralization of the approach and at‐home self‐sampling. Although the benefits of microsampling have been recognised, its adoption in clinical practice has been slow. Several microsampling technologies and devices are currently available and employed in research studies for various biomedical applications. This review provides an overview of the state‐of‐the‐art in microsampling technology with a focus on the latest developments and advancements in the field of microsampling. Research published in the year 2022, including studies (i) developing strategies for the quantitation of analytes in microsamples and (ii) bridging and comparing the interchangeability between matrices and choice of technology for a given application, is reviewed to assess the advantages, challenges and limitations of the current state of microsampling. Successful implementation of microsampling in routine clinical care requires continued efforts for standardization and harmonization. Microsampling has been shown to facilitate data‐rich studies and a patient‐centric approach to healthcare and is foreseen to play a central role in the future digital revolution of healthcare through continuous monitoring to improve the quality of life.

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Section: Sub-classificationmentioning

confidence: 85%

Section: Microsampling Technologies and Innovationsmentioning

confidence: 97%

Section: Applicationsmentioning

confidence: 99%

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Blood microsampling technologies: Innovations and applications in 2022

Thangavelu

Wouters

Kindt

et al. 2023

Analytical Science Advances

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“…In general, for protein determinations and proteomic analyses, mainly aqueous extraction solvents are used. Conventional solvents like ammonium bicarbonate buffers can be used for tryptic digestion of most of the proteins [24,25]. For hydrophobic proteins, sodium bicarbonate can be used for solubilization and consecutive precipitation before bottom-up treatment [26].…”

Section: Dbs: Materials and Proceduresmentioning

confidence: 99%

Dried blood spot analysis with liquid chromatography and mass spectrometry: Trends in clinical chemistry

Skogvold

Rootwelt

Reubsaet

et al. 2023

J of Separation Science

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Dried blood spot samples are simple to prepare and transport, enabling safe and accessible diagnostics, both locally and globally. We review dried blood spot samples for clinical analysis, focusing on liquid chromatography‐mass spectrometry as a versatile measurement tool for these samples. Dried blood spot samples can provide information for, for example, metabolomics, xenobiotic analysis, and proteomics. Targeted analyses of small molecules are the main application of dried blood spot samples and liquid chromatography‐mass spectrometry, but emerging applications include untargeted metabolomics and proteomics. Applications are highly varied, including analyses related to newborn screening, diagnostics and monitoring of disease progression and treatment effects of virtually any disease, as well as studies into the physiology and effects of diet, exercise, xenobiotics, and doping. A range of dried blood spot products and methods are available, and applied liquid chromatography‐mass spectrometry instrumentation is varied with regard to liquid chromatography column formats and selectivity. In addition, novel approaches such as on‐paper sample preparation (e.g., selective trapping of analytes with paper‐immobilized antibodies) are described. We focus on research papers published in the last 5 years.

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“…Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) is always a fit-for-purpose analytical tool in response to a peptide mapping strategy. 14–16 LC is responsible for chromatographing the proteolytic peptide pool into pure fractions and subsequently transmitting to MS/MS for the acquisition of both MS 1 and MS 2 spectra for each peptide. Afterwards, those quasi-molecular ions, e.g.…”

Section: Introductionmentioning

confidence: 99%