Development of an online solid-phase extraction-liquid chromatography-mass spectrometric analysis of oxcarbazepine and its active metabolite licarbazepine from plasma with a direct injection step

Qu, Lihua; Pan, Lixuan; Wang, Li; Liu, Congmin; Tian, Yao; Hao, Zhihui

doi:10.1016/j.jchromb.2019.06.037

Cited by 4 publications

(4 citation statements)

References 25 publications

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“…Sample processing in the system consisted of loading, transfer, and separation and has been outlined previously 18 . The six‐pore valve controlled solvent flow between the SPE cartridge and analytical column.…”

Section: Methodsmentioning

confidence: 99%

“…Both SPE cartridge and chromatographic columns were maintained at 30 C. Sample processing in the system consisted of loading, transfer, and separation and has been outlined previously. 18 The six-pore valve controlled solvent flow between the SPE cartridge and analytical column. The connection position and switching time are shown in Figure 2: first step, at 0.0 min the valve was set at positions 1-6 for sample loading on the SPE cartridge; second step, at 2 min the valve was set at positions 1-2 for analytes transferring to the analytical column; final step, at 3 min the valve went back to positions 1-6 for chromatographic separation.…”

Section: Online-spe-lc-hrms Conditionsmentioning

confidence: 99%

“…MS/MS offered considerable advantages, including speed, selectivity, sensitivity, and robustness, and was a highly efficient analytical tool for biological specimens 17 . Recent developments in high‐resolution mass spectrometry (HRMS) had resulted in quantitation abilities comparable to those of MS/MS, besides its high resolving power, high mass accuracy, and high‐performance quadrupole for selectivity 18,19 . Sample pretreatment methods prior to the analytical procedure were similar across methods, such as liquid‐liquid extraction (LLE), 6–8 protein precipitation (PP), 4,9,12,15 and solid‐phase extraction (SPE) 10,11,13,14 as well as dispersive liquid–liquid microextraction 16 .…”

Section: Introductionmentioning

confidence: 99%

“…17 Recent developments in high-resolution mass spectrometry (HRMS) had resulted in quantitation abilities comparable to those of MS/MS, besides its high resolving power, high mass accuracy, and highperformance quadrupole for selectivity. 18,19 Sample pretreatment methods prior to the analytical procedure were similar across methods, such as liquid-liquid extraction (LLE), [6][7][8] protein precipitation (PP), 4,9,12,15 and solid-phase extraction (SPE) 10,11,13,14 as well as dispersive liquid-liquid microextraction. 16 These traditional techniques are time-consuming and labor-intensive especially for a large number of samples.…”

Section: Introductionmentioning

confidence: 99%

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An automated streamlined method for the therapeutic drug monitoring of digoxin based on the online‐solid‐phase extraction liquid chromatography tandem high‐resolution mass spectrometry

Liu

Wang

Pan

et al. 2021

Rapid Comm Mass Spectrometry

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Rationale Digoxin is widely used in the clinical treatment of cardiovascular diseases. However, due to its extremely narrow therapeutic window, therapeutic drug monitoring (TDM) is vitally important. In consideration of the time‐consuming and labor‐intensive nature of the traditional techniques, an automated and efficient method was required for the clinical individualized TDM of digoxin. Methods An online solid‐phase extraction liquid chromatography tandem high‐resolution mass spectrometry (online‐SPE‐LC‐HRMS) method was developed and applied for the determination of digoxin in plasma. The online SPE‐LC steps included pretreatment and separation of plasma samples that were carried out using a Waters Oasis HLB cartridge and XBridge Shield RP18 column, respectively. A high‐resolution Q Orbitrap mass spectrometer with targeted‐selected ion monitoring in negative scan mode was applied to monitor formate‐adduct ions [M + HCOO]− m/z 825.42781 for digoxin. Results Linearity was shown over the range 0.1–10 ng mL−1 for digoxin with correlation coefficients of R2 > 0.999. The lower limit of quantitation (LLOQ) for digoxin was 0.1 ng mL−1. Extraction recoveries ranged from 82.61% to 94.28% for digoxin. The intra‐ and inter‐day precision values were < 5.53% with accuracy ranging from 84.97% to 96.75%. The total running time was 10 min for each sample. Conclusion The established method displayed satisfactory recoveries, accuracy, precision, and stability, and successfully applied on the TDM of digoxin. This automated streamlined method provides a powerful tool to guide the individualized administration of digoxin, which is significant for the practice of precision medicine.

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Section: Methodsmentioning

confidence: 99%

Section: Online-spe-lc-hrms Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

An automated streamlined method for the therapeutic drug monitoring of digoxin based on the online‐solid‐phase extraction liquid chromatography tandem high‐resolution mass spectrometry

Liu

Wang

Pan

et al. 2021

Rapid Comm Mass Spectrometry

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Interpretation of tandem mass spectra of antiepileptic drugs using accurate-m/z data and m/z-shifts with stable-isotope labeled analogues

Niessen¹

2020

International Journal of Mass Spectrometry

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Determination of Antiepileptics in Biological Samples—A Review

Martinho,

Simão,

Barroso

et al. 2024

Molecules

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Epilepsy remains a disease that affects many people around the world. With the development of new drugs to treat this condition, the importance of therapeutic drug monitoring continues to rise and remains a challenge for the medical community. This review article explores recent advances in the detection of antiepileptic drugs across various sample types commonly used for drug monitoring, with a focus on their applications and impact. Some of these new methods have proven to be simpler, greener, and faster, making them easier to apply in the context of therapeutic drug monitoring. Additionally, besides the classic use of blood and its derivatives, there has been significant research into the application of alternative matrices due to their ease of sample collection and capacity to reflect drug behavior in blood. These advances have contributed to increasing the efficacy of therapeutic drug monitoring while enhancing its accessibility to the population.

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Development of an online solid-phase extraction-liquid chromatography-mass spectrometric analysis of oxcarbazepine and its active metabolite licarbazepine from plasma with a direct injection step

Cited by 4 publications

References 25 publications

An automated streamlined method for the therapeutic drug monitoring of digoxin based on the online‐solid‐phase extraction liquid chromatography tandem high‐resolution mass spectrometry

An automated streamlined method for the therapeutic drug monitoring of digoxin based on the online‐solid‐phase extraction liquid chromatography tandem high‐resolution mass spectrometry

Interpretation of tandem mass spectra of antiepileptic drugs using accurate-m/z data and m/z-shifts with stable-isotope labeled analogues

Determination of Antiepileptics in Biological Samples—A Review

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