Development of an SPE–HPLC–MS method for simultaneous determination and pharmacokinetic study of bioactive constituents of Yu Ping Feng San in rat plasma after oral administration

Li, Tao; Wang, Yiwei; Wang, Yanli; Liang, Rixin; Zhang, Dong; Zhang, Huihui; Chen, Li; Yang, Weipeng

doi:10.1016/j.jep.2012.12.010

Cited by 17 publications

(9 citation statements)

References 30 publications

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“…In pharmacokinetic studies, oral administration of a cimifugin-containing crude drug to rats resulted in a high concentration of cimifugin in the blood [ 13 , 14 ]. For instance, cimifugin was measured at 881–1510 ng/mL in the blood of rats given a traditional Chinese medicine (Yu Ping Feng San) [ 33 ]. Cimifugin suppressed nitric oxide production by murine macrophages and exhibited 1,1-diphenyl-2-picrylhydrazyl free-radical scavenging activity in a cell-free bioassay system [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Suppression ofPropionibacterium acnes-Induced Dermatitis by a Traditional Japanese Medicine, Jumihaidokuto, Modifying Macrophage Functions

Sekiguchi

Koseki

Tsuchiya

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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Purpose. Macrophages serve as sweepers of microbes and inflammation-derived wastes and regulators of inflammation. Some traditional Japanese medicines are reported to have adjuvant effects by modifying macrophages. Our aim was to characterize the actions of jumihaidokuto (JHT) for treatment of skin inflammations including acne vulgaris, in which Propionibacterium acnes has pathogenic roles. Methods. Dermatitis was induced in rat ears by intradermal injection of P. acnes. JHT or prednisolone (PDN) was given orally, and ear thickness and histology were evaluated. The effects of constituents and metabolites of JHT on monocytes were tested by cell-based assays using the human monocytic THP-1 cell. Results. JHT and PDN suppressed the ear thickness induced by P. acnes injection. Histological examinations revealed that JHT, but not PDN, promoted macrophage accumulation at 24 h after the injection. PDN suppressed the macrophage chemokine MCP-1 in the inflamed ears, while JHT did not affect it. The JHT constituents liquiritigenin and isoliquiritin increased expression of CD86 (type-1 macrophage marker) and CD192 (MCP-1 receptor) and enhanced phagocytosis by THP-1. Conclusions. JHT suppressed dermatitis, probably by enhancing type-1 macrophage functions, with an action different from PDN. JHT may be a beneficial drug in treatment of skin inflammation induced by P. acnes.

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Section: Discussionmentioning

confidence: 99%

Suppression ofPropionibacterium acnes-Induced Dermatitis by a Traditional Japanese Medicine, Jumihaidokuto, Modifying Macrophage Functions

Sekiguchi

Koseki

Tsuchiya

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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“…The rats were fasted overnight and injected a dose of 132.8 mg/kg. Blood samples (about 2.0 mL) from intraocular angular vein of rats were collected and placed into EDTA-2Na anticoagulant tubes at predetermined interval times (5,10,20,30,60,90,120,180, and 240 min). Then, blood samples were centrifuged at 3800 rpm for 10 min, and the separated plasma samples were stored at −20 ∘ C for next analysis.…”

Section: Animal Administration and Samplingmentioning

confidence: 99%

“…It can explain the variation of the intensity of the pharmacological effects and explore many in vivo key issues during a pharmacokinetics study, such as absorption and distribution of chemical drugs, bioavailability, biotransformation and excretion pathway. As for TCM, only one or more effective components with known chemical structures in mixtures were selected as targets to investigate the pharmacokinetics by determining the individual metabolomic component concentration in the blood, which is the same as the method of western chemical drugs' pharmacokinetics studies [7][8][9][10]. Even if a single herb of TCM contains dozens or hundreds of kinds of chemical components [11,12], not to mention the Chinese herbal formula [13,14], some individual metabolomic components' in vivo tests cannot represent pharmacokinetics process of TCM with complicated multicomponents and cannot clarify the relationship between a variety of components and integral efficacy [15].…”

Section: Introductionmentioning

confidence: 99%

A New Method of Area under the Absorbance-Wavelength Curve for Rats Total Metabolomic Pharmacokinetics from Yangxue Injection with Multicomponents

Zhou

Xiao

Yang

et al. 2013

Journal of Spectroscopy

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To bridge the convergence between traditional Chinese medicine (TCM) and modern medicine originated from the West, a new method of area under the absorbance-wavelength curve (AUAWC) by spectrophotometer scanning was investigated and compared with HPLC method to explore metabolomic pharmacokinetics in rats. AUAWC and drug total concentration were obtained after Yangxue was injected to rats. Meanwhile, individual concentrations of sodium ferulate, tetramethylpyrazine hydrochloride, tanshinol sodium, and sodium tanshinone IIA sulfonate in plasma were determined by HPLC. Metabolomic profile of multicomponents plasma concentration time from AUAWC and that of individual components from HPLC were compared. The data from AUAWC had one-compartment model with mean area under concentration versus time (AUC) of 9370.58 min·μg/mL and mean elimination half-life (t1/2) of 12.92 min. The results by HPLC demonstrated that sodium ferulate and tetramethylpyrazine hydrochloride had one-compartment model with AUC of 6075.50 and 876.94 min·μg/mL,t1/2of 10.85 and 20.57 min, respectively. Tanshinol sodium and sodium tanshinone IIA sulfonate showed two-compartment model, and AUC was 29.58 and 201.46 witht1/2βof 1.76 and 16.90, respectively. The profiles indicated that method of AUAWC can be used to study pharmacokinetics of TCM with multicomponents and to improve its development of active theory and application in clinic combined with in vivo metabolomic profile of HPLC.

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“…Some analytical techniques based on LC-MS have been reported for the detection of several high-abundance components and their counterparts in YPF in biological samples [ 21 , 22 ]. Most of these reports are focused on one kind of compound in single herbal plant of YPF, especially Astragali radix and Saposhnikoviae radix , using the most abundant compounds such as saponins and their aglycones as chemical markers [ 23 ]. Only a small number of reports are available on the simultaneous analysis of the bioactive constituents in Atractylodis rhizoma , such as atractylenolide I–III [ 24 , 25 , 26 ], as its low content and similar retention behaviors make the detection difficult.…”

Section: Introductionmentioning

confidence: 99%

Using UPLC-MS/MS for Characterization of Active Components in Extracts of Yupingfeng and Application to a Comparative Pharmacokinetic Study in Rat Plasma after Oral Administration

et al. 2017

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Yupingfeng (YPF), a famous traditional Chinese medicine, which contains a large array of compounds, has been effectually used in health protection. A two-dimensional liquid chromatography (2D-LC) combined with quadrupole time-of-flight mass spectrometry (QTOF-MS) method was firstly established to separate and identify chemical components in YPF. A total of 33 compounds were identified, including 15 constituents (flavonoids and saponins) in Astragali radix; seven constituents (sesquiterpenoids and polysaccharide) in Atractylodis rhizoma; and 11 constituents (chromone and coumarins) in Saposhnikoviae radix. The corresponding fragmentation pathway of typical substances was investigated. Then, seven active constituents (astragaloside, calycosin, formononetin, cimicifugoside, 4-O-beta-d-glucosyl-5-O-methylvisamminol, sec-O-glucosylhamaudol, and atractylenolide II) derived from three medicinal plants were chosen to further investigate the pharmacokinetic behavior of YPF formula using ultrahigh-performance liquid chromatography with triple quadrupole mass spectrometry system. The method was sensitive, accurate and reliable. We also used the area under the plasma concentration–time curve from zero to infinity (AUC0−∞) as weighting factor to make an integrated pharmacokinetic curve. Results show that the constituents of Saposhnikoviae radix have the best absorption and pharmacokinetic behavior and may play important role in leading to the changes of overall therapeutic effects of YPF. Further study is needed to confirm the association between them.

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Development of an SPE–HPLC–MS method for simultaneous determination and pharmacokinetic study of bioactive constituents of Yu Ping Feng San in rat plasma after oral administration

Abstract: This analytical method is a selective, sensitive, precise, accurate, and reliable assay for simultaneous determination of cycloastragenol, calycosin, formononetin, GMV, and cimifugin in rat plasma.

Cited by 17 publications

References 30 publications

Suppression ofPropionibacterium acnes-Induced Dermatitis by a Traditional Japanese Medicine, Jumihaidokuto, Modifying Macrophage Functions

Suppression ofPropionibacterium acnes-Induced Dermatitis by a Traditional Japanese Medicine, Jumihaidokuto, Modifying Macrophage Functions

A New Method of Area under the Absorbance-Wavelength Curve for Rats Total Metabolomic Pharmacokinetics from Yangxue Injection with Multicomponents

Using UPLC-MS/MS for Characterization of Active Components in Extracts of Yupingfeng and Application to a Comparative Pharmacokinetic Study in Rat Plasma after Oral Administration

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