Development of Analytical Method for Picoxystrobin in Agricultural Commodities Using GC/ECD and GC/MS

Kwon, Hye-Young; Kim, Chan-Sup; Park, Byung‐Jun; Kim, Il-Hwan; Hong, Su‐Myeong; Son, Kyung-Ae; Jin, Yong-Duk; Lee, Je-Bong; Im, Geon-Jae; Kim, Doo-Ho

doi:10.5338/kjea.2012.31.2.146

Cited by 5 publications

(2 citation statements)

References 5 publications

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“…So far, few reports have been published for the determination of picoxystrobin in different matrices. For instance, Kwon et al [11] developed an analytical method for the determination of picoxystrobin in apple, green pepper, potato, hulled rice, and soybean using gas chromatography (GC)-electron capture detection (ECD) and GC-mass spectrometry (MS). In their study, they extracted the samples using acetone, then partitioned into n-hexane using brine, and undertook further cleanup with Florisil SPE cartridges.…”

Section: Introductionmentioning

confidence: 99%

Residue analysis of picoxystrobin in oriental melon using gas chromatography coupled with electron capture detection and mass spectrometric confirmation: application to dissipation kinetics and risk assessment

Kabir

Rahman

El‐Aty

et al. 2017

Food Sci Biotechnol

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This study was carried out to determine the residual amounts of picoxystrobin in oriental melon ( L.) grown under plastic house conditions at two different sites. Samples collected over 10 days were extracted using acetonitrile and salting out (using solid sodium chloride) and purified using Florisil SPE cartridges. The analyte was determined using GC-ECD and field-incurred residues were verified using GC-MS. The calibration curve was linear over the range 0.02-2.0 mg/L with a = 0.9998. The LOD and LOQ were 0.003 and 0.01 mg/kg, respectively. Recoveries, tested at three spiking levels, were satisfactory with rates in the range 87.7-101.5% and relative standard deviations ≤9.6. The dissipation half-lives were 3.4 and 3.7 days for sites 1 and 2, respectively. Hazard estimates obtained using hazard quotients revealed no health risk from the suggested pesticide application dosage when considering an adult's body weight, oriental melon consumption, and the acceptable daily intake of picoxystrobin.

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Section: Introductionmentioning

confidence: 99%

Residue analysis of picoxystrobin in oriental melon using gas chromatography coupled with electron capture detection and mass spectrometric confirmation: application to dissipation kinetics and risk assessment

Kabir

Rahman

El‐Aty

et al. 2017

Food Sci Biotechnol

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“…Currently, instrumental methods, including gas chromatography-tandem mass spectrometry (GC-MS/MS) [10,11] and liquid chromatography-tandem mass spectrometry (LC-MS/MS) [12,13], are the primary approaches used for detecting picoxystrobin. These methods are widely used because of their high sensitivity, specificity, and accuracy [14].…”

Section: Introductionmentioning

confidence: 99%

Development of a Time-Resolved Fluorescent Microsphere Test Strip for Rapid, On-Site, and Sensitive Detection of Picoxystrobin in Vegetables

Chen,

Zhang

et al. 2024

Foods

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Picoxystrobin (PIC) is a fungicide extensively used for disease control in both crops and vegetables. Residues of PIC in vegetables pose a potential threat to human health due to their accumulation in the food chain. In this study, a specific PIC monoclonal antibody (mAb) was developed by introducing a carboxylic acid arm into PIC and subsequently preparing a hapten and an artificial antigen. A sensitive and rapid time-resolved fluorescence immunochromatographic assay (TRFICA) was established based on the mAb. Subsequently, using a time-resolved fluorescent microsphere (TRFM) as signal probe, mAbs and microspheres were covalently coupled. The activated pH, the mAb diluents, the mAb amount, and the probe amount were optimized. Under optimized conditions, the quantitative limits of detection (qLOD) of PIC in cucumber, green pepper, and tomato using TRFICA were established at 0.61, 0.26, and 3.44 ng/mL, respectively; the 50% inhibiting concentrations (IC50) were 11.76, 5.29, and 37.68 ng/mL, respectively. The linear ranges were 1.81–76.71, 0.80–35.04, and 8.32–170.55 ng/mL, respectively. The average recovery in cucumber, green pepper, and tomato samples ranged from 79.8% to 105.0%, and the corresponding coefficients of variation (CV) were below 14.2%. In addition, 15 vegetable samples were selected and compared with the results obtained using ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS). The results revealed a high degree of concordance between the proposed method and UPLC-MS/MS. In conclusion, the devised TRFICA method is a valuable tool for rapid, on-site, and highly sensitive detection of PIC residues in vegetables.

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Dissipation and residues of picoxystrobin in peanut and field soil by QuEChERS and HPLC–MS/MS

Zhu

Feng

et al. 2015

Environ Monit Assess

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The dissipation and final residues of picoxystrobin in peanut and soil were determined by a modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) method and high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). The dissipation and final residue of picoxystrobin at three different provinces (Hebei, Hubei, and Shandong) in China were studied. The fortification experiments at three different spiking levels of 0.01, 0.05, and 0.5 mg kg(-1) in all matrices (soil, peanut seedling, shell, stalk, and kernels) were conducted, and the recoveries were 79-114% with relative standard deviations of 3-12 (n = 5). The dissipation half-lives of picoxystrobin were 1.5-8.6 days in soil, and 2.1-2.8 days in seedlings. The final residues of picoxystrobin in supervised field trials were 0.05-6.82 mg kg(-1) in stalk, ≤0.381 mg kg(-1) in soil, ≤0.069 mg kg(-1) in shells, and ≤0.005 mg kg(-1) in peanut kernels. Considering the final residue levels and the maximum residue limits (MRLs), the pre-harvest interval of 14 days was recommended for the safe use of picoxystrobin in peanut crop.

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Development of Analytical Method for Picoxystrobin in Agricultural Commodities Using GC/ECD and GC/MS

Cited by 5 publications

References 5 publications

Residue analysis of picoxystrobin in oriental melon using gas chromatography coupled with electron capture detection and mass spectrometric confirmation: application to dissipation kinetics and risk assessment

Residue analysis of picoxystrobin in oriental melon using gas chromatography coupled with electron capture detection and mass spectrometric confirmation: application to dissipation kinetics and risk assessment

Development of a Time-Resolved Fluorescent Microsphere Test Strip for Rapid, On-Site, and Sensitive Detection of Picoxystrobin in Vegetables

Dissipation and residues of picoxystrobin in peanut and field soil by QuEChERS and HPLC–MS/MS

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