Development of Analytical Methods for the Estimation of Alpinetin in Seeds of Amomum subulatum Roxb

Singh, M; Kamal, YT; Tamboli, ET; Parveen, R; Siddiqui, KM; Ahmad, Shair

doi:10.1055/s-0031-1273618

Planta Med

2011

DOI: 10.1055/s-0031-1273618

|View full text |Cite

Development of Analytical Methods for the Estimation of Alpinetin in Seeds of Amomum subulatum Roxb

M Singh¹,

YT Kamal²,

ET Tamboli³

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2015

Publication Types

Select...

Article1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In spite of valuable medicinal uses of the drug, there are no analytical methods reported for its quality control and analysis alpinetin. Hence, in the present investigations, simple, accurate and reliable HPLC and HPTLC methods were developed [17] for Research Paper the analysis of its important constituent alpinetin in A. subulatum seed extracts. The sample preparation is the valuable factor to analyse exact content of any constituent in crude herbal drugs.…”

mentioning

confidence: 99%

Matrix solid-phase dispersion extraction and quantification of alpinetin in amomum seed using validated HPLC and HPTLC methods

Singh¹,

Kamal²,

Khan³

et al. 2015

Indian J Pharm Sci

View full text Add to dashboard Cite

Singh, et al.: Extraction and Quantification of Alpinetin in Amomum Seeds Alpinetin is a flavonoidal constituent of seeds of Amomum subulatum Roxb., recently reported to possess vasorelaxant and antiHIV activities. Simple, accurate and precise HPLC and HPTLC methods were developed for the analysis of alpinetin in A. subulatum seed extracts and extraction technique was optimized to get maximum yield using conventional, ultrasonic and matrix solid phase dispersion extraction. HPLC was performed on a C18 column with methanol and water (70:30, v/v) as mobile phase at a flow rate of 1.0 ml/min whereas HPTLC on silica aluminum sheet (60F 254 ) using toluene, dichloromethane and ethyl acetate as solvent system. A sharp peak was obtained for alpinetin at a retention time (R t ) of 5.7 min by HPLC and retardation factor (R f ) of 0.48 by HPTLC. Both methods were validated as per the ICH guidelines and the content of alpinetin was estimated in different extracts. Matrix solid phase dispersion technique was found most suitable for extracting alpinetin as compared to other techniques. Validation data are indicative of good precision and accuracy and proved the reliability of the methods.

show abstract

mentioning

confidence: 99%

Matrix solid-phase dispersion extraction and quantification of alpinetin in amomum seed using validated HPLC and HPTLC methods

Singh¹,

Kamal²,

Khan³

et al. 2015

Indian J Pharm Sci

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Development of Analytical Methods for the Estimation of Alpinetin in Seeds of Amomum subulatum Roxb

Cited by 1 publication

References 0 publications

Matrix solid-phase dispersion extraction and quantification of alpinetin in amomum seed using validated HPLC and HPTLC methods

Matrix solid-phase dispersion extraction and quantification of alpinetin in amomum seed using validated HPLC and HPTLC methods

Contact Info

Product

Resources

About