Development of antibiotic resistance in<i>Mycoplasma gallisepticum in vitro</i>

Zanella, A.; Martino, Piera Anna; Pratelli, Annamaria; Stonfer, M.

doi:10.1080/03079459808419389

Cited by 22 publications

(13 citation statements)

References 14 publications

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“…In the present study, high-level resistance to erythromycin developed in M. gallisepticum after 5-6 passages. This result is in general agreement with previous findings [16,17]. Unexpectedly, however, all of the erythromycinresistant mutants obtained in our study exhibited crossresistance to tylosin, tilmicosin, and lincomycin.…”

Section: Discussionsupporting

confidence: 94%

Induction of macrolide resistance inMycoplasma gallisepticumin vitro and its resistance-related mutations within domain V of 23S rRNA

Yi-bao

et al. 2005

FEMS Microbiology Letters

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Antibiotic-resistant mutants of Mycoplasma gallisepticum were selected in vitro from the susceptible strains S6 and BG44T by serial passages in stepwise concentrations of erythromycin, tylosin, or tilmicosin. High resistance to erythromycin or tilmicosin developed readily, whereas resistance to tylosin developed only after greater numbers of passages. Three mutants selected by each selector antibiotic were cloned and detected, and all cloned mutants exhibited cross-resistance to the three selector antibiotics as well as to lincomycin. Portions of the genes encoding domain V of 23S rRNA of the cloned mutants were amplified by PCR, and their nucleotide sequences were compared to those of the susceptible parent strains. Five of the six mutants selected by erythromycin harbored an A2058G (Escherichia coli numbering) mutation in one of the two 23S rRNA. One of the six mutants selected by erythromycin harbored a G2057A mutation and an A2059G mutation in the other 23S rRNA. In tilmicosin-selected mutants, two mutations, A2058G and A2503U, occurred in one of the two 23S rRNA. No mutation was detected in the two 23S rRNA of tylosin-selected mutants with low-level resistance. Mutations at homologous locations in the 23S rRNA of other macrolide-resistant bacteria indicate that the phenotype of macrolide resistance occurring in M. gallisepticum is strongly associated with point mutations in domain V of 23S rRNA.

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Section: Discussionsupporting

confidence: 94%

Induction of macrolide resistance inMycoplasma gallisepticumin vitro and its resistance-related mutations within domain V of 23S rRNA

Yi-bao

et al. 2005

FEMS Microbiology Letters

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“…This could be attributed to the function of tylosin. Previous research has reported that the MIC of ofloxacin is up-regulated in erythromycin-induced strain [48] and the MIC of chlortetracycline increased 64 times in the tylosininduced E. coli [49]. In our experiment, the MICs of terramycin, chlortetracycline, ofloxacin and enrofloxacin were shown high-level resistance in the S-t-R 128 strain.…”

Section: Discussionsupporting

confidence: 52%

Analysis of multidrug resistance in Streptococcus suis ATCC 700794 under tylosin stress

et al. 2019

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Streptococcus suis is an important zoonotic pathogen. The massive use of tylosin and other antibiotics in swine production has led to the emergence of resistant phenotypes of S. suis. However, there are no adequate measures available to address the problem of bacterial resistance. This study involved the use of 1/4 MIC (0.125 µg/mL) of tylosin to investigate resistance-related proteins by S. suis ATCC 700794. Our results showed that 171 proteins were differentially expressed in S. suis tested with 1/4 MIC (0.125 µg/mL) of tylosin using iTRAQ-based quantitative proteomic methods. TCS, heat shock protein and elongation factors were differentially expressed at 1/4 MIC (0.125 µg/mL) of tylosin compared to non treated, control cells. Using quantitative RT-PCR analysis, we verified the relationship between the differentially expressed proteins in S. suis with different MIC values. The data showed that expression profile for elongation factor G (fusA), elongation factor Ts (tsf), elongation factor Tu (tuf), putative histidine kinase of the competence regulon, ComD (comD), putative competence-damage inducible protein (cinA) and protein GrpE (grpE), observed in tylosin-resistant S. suis, correlated with that of S. suis ATCC 700794 at 1/4 MIC (0.125 µg/mL). The MIC of tylosin-resistant showed high-level resistance in terramycin, chlortetracycline, ofloxacin and enrofloxacin. Our findings demonstrated the importance of elongation factors, TCS and heat shock protein during development of tylosin resistance in S. suis. Thus, our study will provide insight into new drug targets and help reduce bacterial multidrug resistance through development of corresponding inhibitors.

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“…Herein, all M. gallisepticum isolates showed excellent susceptibilities to tylosin (0.0625-4 µg/mL) and tiamulin (0.031-2 µg/mL); meanwhile, 35.7% of recovered isolates exhibited erythromycin resistance. Generally, a previous study reported that tilmicosin and tylosin are effective against M. gallisepticum infection, and resistance of the microorganism to tylosin has been reported to develop slower than resistance to erythromycin [28].…”

Section: Discussionmentioning

confidence: 99%

Mutations of domain V in 23S ribosomal RNA of macrolide-resistant Mycoplasma gallisepticum isolates in Egypt

Ammar

El-Aziz

Gharib

et al. 2016

J Infect Dev Ctries

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Introduction: Avian mycoplasmas impose a significant economic burden to the poultry industry. In recent years, macrolide-resistant Mycoplasma gallisepticum have occasionally been encountered in Egypt. Methodology: This study was designed to document the involvement of macrolide-resistant M. gallisepticum in respiratory organs of chickens suffering respiratory problems. Concurrently, an exhaustive molecular characterization of the intrinsic resistance of recovered isolates to macrolides was done. Results: Of 120 chickens showing respiratory problems, 14 (11.67%) M. gallisepticum were isolated and genetically identified; 8 of them were recovered from air sacs, 4 from lungs, and 2 from tracheas. Broth microdilution of all M. gallisepticum isolates showed various degrees of minimum inhibitory concentrations (MICs) against macrolides: erythromycin (0.25-32 µg/mL), tylosin (0.0625-4 µg/mL), and tiamulin (0.031-2 µg/mL). Nucleotide sequencing of domain V (peptidyl transferase region) of the 23S rRNA gene of macrolide-resistant M. gallisepticum isolates revealed transition mutations at positions 2068 and 2069 (corresponding to 2058 and 2059 in Escherichia coli numbering) in an isolate and at position 2067 (corresponding to 2057 in E. coli numbering) in three isolates as hot spots for macrolide resistance. Surprisingly, a transversion mutation at position 2621 (corresponding to 2611 in E. coli numbering) was reported in one of the recovered isolates as a first report. Conclusion: Generation of new mutations is evidence for persistence of M. gallisepticum despite macrolide treatment. Periodic surveys to monitor for the possible appearance of resistant strains are recommended.

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Development of antibiotic resistance inMycoplasma gallisepticum in vitro

Cited by 22 publications

References 14 publications

Induction of macrolide resistance inMycoplasma gallisepticumin vitro and its resistance-related mutations within domain V of 23S rRNA

Induction of macrolide resistance inMycoplasma gallisepticumin vitro and its resistance-related mutations within domain V of 23S rRNA

Analysis of multidrug resistance in Streptococcus suis ATCC 700794 under tylosin stress

Mutations of domain V in 23S ribosomal RNA of macrolide-resistant Mycoplasma gallisepticum isolates in Egypt

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