Development of bioanalytical HPLC method for simultaneous determination of the antialzhiemer, donepezil hydrochloride and the antidepressant, citalopram hydrobromide in raw materials, spiked human plasma and tablets dosage form

Selective LC–MS/MS determination of citalopram enantiomers and application to a pharmacokinetic evaluation of generic and reference formulations

“…As observed, stationary phases consisted of silica chemically bound to n-alkyl groups (C18, CN, Select B) (Bhimanadhuni et al, 2012;De Sousa et al, 2012;El-Gindy et al, 2006;Malfará et al, 2007;Peng et al, 2016;Ragab & Bahgat, 2019;Skibi nski & Misztal, 2007), chiral selectors (β-cyclodextrin or cellulose derivatives) (Geryk et al, 2013;Haupt, 1996;Hegstad et al, 2017;Kosel et al, 1998;Rahman et al, 2017;Rocha et al, 2007;et al, 1995a, 1995bWeisskopf et al, 2016), and sol-gel capillary monolith (Johannesson & Bergquist, 2007). The HPLC coupled to FL (Kosel et al, 1998;Rochat et al, 1995aRochat et al, , 1995b, UV (Bhimanadhuni et al, 2012;El-Gindy et al, 2006;Geryk et al, 2013;Haupt, 1996;Malfará et al, 2007;Peng et al, 2016;Ragab & Bahgat, 2019;Rahman et al, 2017;Skibi nski & Misztal, 2007;Zheng et al, 2000), or MS/MS detectors (De Sousa et al, 2012;Golovko et al, 2016;Hegstad et al, 2017;Rocha et al, 2007;Weisskopf et al, 2016) was applied to quantify the (R/S)-CTP and/or the enantiomers in plasma, milk, soil, tissues, raw material, and formulations. Additional analytical techniques such as ultra-high performance supercritical fluid chromatography-MS/MS (Hegstad et al, 2017) and capillary electrophoresis instrument coupled to time-of-fligh...…”

“…The HPLC methods with UV or FL detection described until now (Table 5) were applied to assess the quality of the raw materials and drug formulations (Bhimanadhuni et al, 2012;El-Gindy et al, 2006;Geryk et al, 2013;Peng et al, 2016;Ragab & Bahgat, 2019;Rahman et al, 2017), as well as the drug plasma concentrations (Haupt, 1996;Kosel et al, 1998;Malfará et al, 2007;Ragab & Bahgat, 2019;Rochat et al, 1995aRochat et al, , 1995bZheng et al, 2000). However, their longer running time, lower sensitivity, and selectivity compared to the LC-MS/MS methods may limit their applications in pharmacokinetic studies, specially due the long halflife of (R/S)-CTP (Pollock, 2001) and (S)-CTP (Pastoor & Gobburu, 2014).…”

“…The quantification of the racemic and enantiomeric forms of CTP in biological fluids is of great value to support pharmacokinetic studies, including bioequivalence tests, therapeutic drug monitoring (TDM), and populational pharmacokinetic analyses (Agency, 2010;Unceta et al, 2011). The first bioanalytical methods for CTP were conducted using the high-performance liquid chromatograph equipped with UV or fluorescence (FL) detectors employing distinct stationary phases and techniques of sample treatment, such as liquidliquid extraction (LLE), solid-phase extraction, and online sample purification (Haupt, 1996;Kosel et al, 1998;Malfará et al, 2007;Ragab & Bahgat, 2019;Rochat et al, 1995aRochat et al, , 1995bUnceta et al, 2011;Zheng et al, 2000).…”

Investigating the Utility of Fabric Phase Sorptive Extraction and HPLC-UV-Vis/DAD to Determine Antidepressant Drugs in Environmental Aqueous Samples

“…The literature has documented numerous analytical techniques for the determination of ADs. The most commonly used are high performance liquid chromatography (HPLC) coupled to UV-Vis or a mass spectrometric detector (MS) [6][7][8][9][10][11][12][13]. Gas chromatography (GC) coupled to MS [14][15][16][17] or tandem MS [18], and the capillary electrophoretic technique (CE) have also been reported [19].…”

Donepezil and Quercetin Simultaneous Estimation in Rat Plasma Using Developed Bioanalytical HPLC Method: Relevance in Pharmacokinetic Studies

“…[5][6][7][8][9] Memantine, galantamine, and rivastigmine have all been determined in combination with DPZ. [10][11][12] Fresh green leafy vegetables, red onions, apples, tomatoes, red grapes, green tea, and black tea all contain the naturally occurring flavonoid quercetin (QT) (Figure 1b). 13 Owing to its anti-inflammatory and antioxidant effects, QT has therapeutic potential for disorders like cardiovascular disease, cancer, a) b) Figure 1: Chemical structure: a) Donepezil; b) Quercetin 21,22…”