Development of CD27<sup>+</sup> marginal zone B cells requires GALT

Yeramilli, Venkata A.; Knight, Katherine L.

doi:10.1002/eji.201243205

Cited by 9 publications

(6 citation statements)

References 35 publications

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“…Additional ILC survival factors such as IL-1β and IL-23 were predominantly expressed by splenic DCs and macrophages, suggesting that ILCs inhabit a splenic niche that entails survival signals from both stromal and immune cells. Of note, MRCs received further activation signals from TLR ligands such as LPS, lending support to the possibility that commensal antigens from the intestinal mucosa help the post-natal development and function of the splenic MZ 4 , 10 , 47 , 48 .…”

Section: Discussionmentioning

confidence: 86%

Innate lymphoid cells integrate stromal and immunological signals to enhance antibody production by splenic marginal zone B cells

et al. 2014

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Innate lymphoid cells (ILCs) regulate stromal, epithelial and immune cells, but their impact on B cells remains unclear. We identified RORγt+ ILCs nearby the marginal zone (MZ), a splenic compartment containing innate-like B cells that respond to circulating T cell-independent (TI) antigens. Spenic ILCs established a bidirectional crosstalk with MAdCAM-1+ marginal reticular cells by providing tumor necrosis factor (TNF) and lymphotoxin, and activated MZ B cells via BAFF, CD40 ligand and the Notch ligand, Delta-like 1. Splenic ILCs further helped MZ B cells and their plasma cell progeny by co-opting neutrophils through the release of GM-CSF. Consequently, ILC depletion impaired both pre- and post-immune TI antibody responses. Thus, ILCs integrate stromal and myeloid signals to orchestrate innate-like antibody production at the interface between the immune and circulatory systems.

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Section: Discussionmentioning

confidence: 86%

Innate lymphoid cells integrate stromal and immunological signals to enhance antibody production by splenic marginal zone B cells

et al. 2014

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“…However, CD27 − memory B-cells have been reported in other species. Most rabbit mucosal B-cells are CD27 − but IgM + [33]. Circulating CD27 − memory B-cells in humans can represent up to one third of IgG1 and nearly half of IgG3 memory B-cells [34].…”

Section: Discussionmentioning

confidence: 99%

Phenotypes and distribution of mucosal memory B-cell populations in the SIV/SHIV rhesus macaque model

Demberg¹,

Mohanram²,

Venzon³

et al. 2014

Clinical Immunology

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As vaccine-elicited antibodies have now been associated with HIV protective efficacy, a thorough understanding of mucosal and systemic B-cell development and maturation is needed. We phenotyped mucosal memory B-cells, investigated isotype expression and homing patterns, and defined plasmablasts and plasma cells at three mucosal sites (duodenum, jejunum and rectum) in rhesus macaques, the commonly used animal model for pre-clinical vaccine studies. Unlike humans, macaque mucosal memory B-cells lacked CD27 expression; only two sub-populations were present: naïve (CD21+CD27−) and tissue-like (CD21−CD27−) memory. Similar to humans, IgA was the dominant isotype expressed. The homing markers CXCR4, CCR6, CCR9 and α4β7 were differentially expressed between naïve and tissue-like memory B-cells. Mucosal plasmablasts were identified as CD19+CD20+/−HLA-DR+Ki-67+IRF4+CD138+/− and mucosal plasma cells as CD19+CD20−HLA-DR−Ki-67−IRF4+CD138+. Both populations were CD39+/−CD27−. Plasma cell phenotype was confirmed by spontaneous IgA secretion by ELISpot of positively-selected cells and J-chain expression by real-time PCR. Duodenal, jejunal and rectal samples were similar in B-cell memory phenotype, isotype expression, homing receptors and plasmablast/plasma cell distribution among the three tissues. Thus rectal biopsies adequately monitor B-cell dynamics in the gut mucosa, and provide a critical view of mucosal B-cell events associated with development of vaccine-elicited protective immune responses and SIV/SHIV pathogenesis and disease control.

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“…This phenomenon may be the cause or consequence of gastrointestinal disease and indicates that spleen and gut are functionally related in humans, at least with respect to this special B‐cell population. In rabbits, there is a clearcut reduction of blood CD27 + B cells, if major parts of the gut‐associated lymphatic tissue are removed …”

Section: Consequences Of Splenectomy and Gut Pathologies In Humansmentioning

confidence: 99%

Human spleen microanatomy: why mice do not suffice

2015

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SummaryThe microanatomical structure of the spleen has been primarily described in mice and rats. This leads to terminological problems with respect to humans and their species-specific splenic microstructure. In mice, rats and humans the spleen consists of the white pulp embedded in the red pulp. In the white pulp, T and B lymphocytes form accumulations, the periarteriolar lymphatic sheaths and the follicles, located around intermediate-sized arterial vessels, the central arteries. The red pulp is a reticular connective tissue containing all types of blood cells. The spleen of mice and rats exhibits an additional well-delineated B-cell compartment, the marginal zone, between white and red pulp. This area is, however, absent in human spleen. Human splenic secondary follicles comprise three zones: a germinal centre, a mantle zone and a superficial zone. In humans, arterioles and sheathed capillaries in the red pulp are surrounded by lymphocytes, especially by B cells. Human sheathed capillaries are related to the splenic ellipsoids of most other vertebrates. Such vessels are lacking in rats or mice, which form an evolutionary exception. Capillary sheaths are composed of endothelial cells, pericytes, special stromal sheath cells, macrophages and B lymphocytes. Human spleens most probably host a totally open circulation system, as connections from capillaries to sinuses were not found in the red pulp. Three stromal cell types of different phenotype and location occur in the human white pulp. Splenic white and red pulp structure is reviewed in rats, mice and humans to encourage further investigations on lymphocyte recirculation through the spleen.

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Development of CD27⁺ marginal zone B cells requires GALT

Cited by 9 publications

References 35 publications

Innate lymphoid cells integrate stromal and immunological signals to enhance antibody production by splenic marginal zone B cells

Innate lymphoid cells integrate stromal and immunological signals to enhance antibody production by splenic marginal zone B cells

Phenotypes and distribution of mucosal memory B-cell populations in the SIV/SHIV rhesus macaque model

Human spleen microanatomy: why mice do not suffice

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Development of CD27+ marginal zone B cells requires GALT

Cited by 9 publications

References 35 publications

Innate lymphoid cells integrate stromal and immunological signals to enhance antibody production by splenic marginal zone B cells

Innate lymphoid cells integrate stromal and immunological signals to enhance antibody production by splenic marginal zone B cells

Phenotypes and distribution of mucosal memory B-cell populations in the SIV/SHIV rhesus macaque model

Human spleen microanatomy: why mice do not suffice

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Development of CD27⁺ marginal zone B cells requires GALT