Development of competitive and noncompetitive immunoassays for clothianidin with high sensitivity and specificity using phage-displayed peptides

You, Tianyang; Yuan, Ding; Chen, He; Song, Guangyue; Huang, Lianrun; Wang, Minghua; Hua, Xiude

doi:10.1016/j.jhazmat.2021.128011

Cited by 25 publications

(11 citation statements)

References 48 publications

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“…The peptide libraries used in this study are displayed on phage pVIII in high-density, whose expressions are controlled by the lac promoter/operator. Such a display strategy may increase the apparent affinity and weaken the growth bias among different phages, thus serving as a footstone for the blended panning strategy using mixed libraries [ 13 ].…”

Section: Resultsmentioning

confidence: 99%

“…The ninety-six-well microplates were purchased from Corning Costar (Corning, NY, USA). The mAb 3D11B12E5 purified by protein A column and phage-displayed cyclic 8, 9, 10-residue random peptide libraries were prepared and stored in our own laboratory [ 13 , 30 ]. The sequencing primer was synthesized by Genscript Bio.…”

Section: Methodsmentioning

confidence: 99%

“…For immunocomplex panning, 100 μL/well IMI standard solution (10 μg/mL) was firstly injected to form immunocomplex for 1 h. After discarding the excess IMI, the mixture of phage-displayed peptide libraries used in peptidomimetic panning was added and incubated at room temperature for another 1 h. After washing, the bound phage was eluted by an elution buffer (0.2 M Gly-HCl containing 1 mg/mL BSA, pH 2.2) for 15 min, and immediately neutralized to pH 7.4 using 1 M Tris-HCl (pH 9.1). The eluted phages were titer-determined and amplified [ 13 ] for subsequent panning.…”

Section: Methodsmentioning

confidence: 99%

“…The noncompetitive format provides a positive correlation signal that makes the detection results more friendly. Unlike open sandwich immunoassays or the noncompetitive immunoassay based on an anti-idiotype antibody, anti-immunocomplex peptide is easier to obtain so that it is increasingly being reported in pesticide residue immunoassay [ 11 , 12 , 13 , 14 ]. These two types of peptides generally improved the sensitivity or specificity of immunoassays in previous reports.…”

Section: Introductionmentioning

confidence: 99%

“…In our recent study, we optimized the pVIII phage display peptide system to improve the panning success rate [ 13 ]. In the present work, IMI was determined as a target and an anti-IMI mAb 3D11B12E5 was employed to screen above the pVIII phage display peptide libraries.…”

Section: Introductionmentioning

confidence: 99%

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Identification and Application of Two Promising Peptide Ligands for the Immunodetection of Imidacloprid Residue

You

Yuan

Huang

et al. 2022

Foods

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As the most widely used neonicotinoid insecticide, it is of great significance to explore the immunoreagents and immunoassays for imidacloprid (IMI) residue. In immunoassays, specific peptide ligands, such as peptidomimetic and anti-immunocomplex peptides, are regarded as promising substitutes for chemical haptens. In the present work, we identified thirty sequences of peptidomimetics and two sequences of anti-immunocomplex peptides for IMI from three phage pVIII display cyclic peptide libraries, in which the anti-immunocomplex peptides are the first reported noncompetitive reagents for IMI. The peptidomimetic 1-9-H and anti-immunocomplex peptide 2-1-H that showed the best sensitivity were utilized to develop competitive and noncompetitive phage enzyme-linked immunosorbent assays (P-ELISAs), with a half inhibition concentration of 0.55 ng/mL for competitive P-ELISA and a half-saturation concentration of 0.35 ng/mL for noncompetitive P-ELISA. The anti-immunocomplex peptide was demonstrated to greatly improve the specificity compared with competitive P-ELISA. In addition, the accuracy of proposed P-ELISAs was confirmed by recovery analysis and HPLC verification in agricultural and environmental samples. These results show that the peptide ligands identified from phage display library can replace chemical haptens in the immunoassays of IMI with the satisfactory performance.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Identification and Application of Two Promising Peptide Ligands for the Immunodetection of Imidacloprid Residue

You

Yuan

Huang

et al. 2022

Foods

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Viral Nanoparticles‐Mediated Delivery of Therapeutic Cargo

Zanganeh

Doroudian

Nowzari

et al. 2023

Advanced Therapeutics

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The rapid advancement of nanotechnology in recent years has opened new avenues of investigation for biomedical sciences. Viral nanoparticles (VNPs) are formulated from plant viruses, mammalian viruses, or bacteriophages. Based on their structure, viruses, and synthetic carriers have been utilized to design bio‐inspired nanocarriers, which serve as building blocks for innovative therapeutic applications. Scientists can chemically or genetically engineer VNPs to encompass various properties, such as enhancing their functionalization with therapeutic molecules and imaging reagents, enabling targeted delivery to specific ligands. The implementation of these novel nanocarrier platforms can revolutionize treatments for cancer, infectious diseases, and chronic illnesses. The primary goal of drug delivery systems is to localize cargo to the specific target site, increasing therapeutic benefits and minimizing off‐target effects. This review critically evaluates the major virus species used as nanocarriers, their applications in therapeutics, and their advantages and disadvantages.

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M13 phage: a versatile building block for a highly specific analysis platform

Wang

Cao

et al. 2023

Anal Bioanal Chem

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Viruses are changing the biosensing and biomedicine landscape due to their multivalency, orthogonal reactivities, and responsiveness to genetic modifications. As the most extensively studied phage model for constructing a phage display library, M13 phage has received much research attention as building blocks or viral scaffolds for various applications including isolation/separation, sensing/probing, and in vivo imaging. Through genetic engineering and chemical modification, M13 phages can be functionalized into a multifunctional analysis platform with various functional regions conducting their functionality without mutual disturbance. Its unique filamentous morphology and flexibility also promoted the analytical performance in terms of target affinity and signal amplification. In this review, we mainly focused on the application of M13 phage in the analytical field and the benefit it brings. We also introduced several genetic engineering and chemical modification approaches for endowing M13 with various functionalities, and summarized some representative applications using M13 phages to construct isolation sorbents, biosensors, cell imaging probes, and immunoassays. Finally, current issues and challenges remaining in this field were discussed and future perspectives were also proposed.

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Development of competitive and noncompetitive immunoassays for clothianidin with high sensitivity and specificity using phage-displayed peptides

Cited by 25 publications

References 48 publications

Identification and Application of Two Promising Peptide Ligands for the Immunodetection of Imidacloprid Residue

Identification and Application of Two Promising Peptide Ligands for the Immunodetection of Imidacloprid Residue

Viral Nanoparticles‐Mediated Delivery of Therapeutic Cargo

M13 phage: a versatile building block for a highly specific analysis platform

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