Development of Cortical Pyramidal Cell and Interneuronal Dendrites: a Role for Kainate Receptor Subunits and NETO1

Jack, Alexander; Hamad, Mohammad I. K.; Gonda, Steffen; Gralla, Sebastian; Pahl, Steffen; Hollmann, Michael; Wahle, Petra

doi:10.1007/s12035-018-1414-0

Cited by 24 publications

(53 citation statements)

References 81 publications

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“…First, we confirmed that young neurons in OTC have functional NMDARs on the cell surface. GFP-transfected OTC were challenged at DIV 10 with 50 µM NMDA to evoke dendritic beading as previously shown (Hamad et al, 2011;Jack et al, 2018). Images of live neurons were taken at 5 min before, or just before (0 min) adding NMDA and subsequently FIGURE 2 | Endogenous NMDA receptors render neurons sensitive to NMDA.…”

Section: Blocking Of Glun2b But Not Glun2a Prevents Nmda-induced Dendmentioning

confidence: 99%

“…OTC were gene gun-transfected as described (Hamad et al, 2011;Jack et al, 2018). All plasmids (Table 1) were prepared as endotoxin-free solutions using the EndoFree Plasmid Maxi Kit (Qiagen, Cat No./ID: 12362).…”

Section: Plasmid Transfectionmentioning

confidence: 99%

“…Finally, we followed the design of our previous studies overexpressing AMPAR and KAR subunits and their accessory proteins (Hamad et al, 2011(Hamad et al, , 2014Jack et al, 2018). We attempted to express plasmids encoding the GluN2A and GluN2B subunit together with EGFP as reporter (for morphometry), or to express plasmids encoding EGFP-tagged GluN2A and EYFP-tagged GluN2B together with mCherry as reporter (for visualization).…”

Section: Pharmacological Treatmentmentioning

confidence: 99%

“…Pyramidal cells and multipolar sparsely spinous interneurons were classified by criteria of dendritic and axonal patterns. Pyramidal neurons were grouped as follows: those of layers II/III have an apical dendrite reaching into layer I, and for layers V/VI we consider those which have an apical dendrite ending in middle layers; thick and thin tufted large layer V pyramidal cells occurred too rarely and were not sampled (Hamad et al, 2014;Jack et al, 2018). Using a ZEISS Axioskop equipped with a discussion bridge we assessed with two trained observers blinded to conditions all completely stained neuronal transfectants per slice culture for symptoms of dendritic injury.…”

Section: Morphological Analysismentioning

confidence: 99%

“…All contribute to dendritic growth (Wong and Ghosh, 2002). For developing neocortical pyramidal neurons selected AMPAR subunits, most effectively as flipspliced variants, are important as well as the KAR GluK2, and both mediate their effects with a contribution by NMDARs (Hamad et al, 2011(Hamad et al, , 2014Jack et al, 2018). The importance of NMDARs has been shown in tadpole tectal neurons which fail to grow when inhibited with 100 µM of the non-selective NMDAR antagonist APV, whereas AMPARs and sodium channel activity were not yet important at these early stages (Rajan and Cline, 1998).…”

Section: Introductionmentioning

confidence: 99%

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GluN2B but Not GluN2A for Basal Dendritic Growth of Cortical Pyramidal Neurons

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Section: Blocking Of Glun2b But Not Glun2a Prevents Nmda-induced Dendmentioning

confidence: 99%

Section: Plasmid Transfectionmentioning

confidence: 99%

Section: Pharmacological Treatmentmentioning

confidence: 99%

Section: Morphological Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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GluN2B but Not GluN2A for Basal Dendritic Growth of Cortical Pyramidal Neurons

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Long‐Lived Organotypic Slice Culture Model of the Rat Basolateral Amygdala

et al. 2021

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Current ProtocolsCurrent Protocols Preparation: Day of dissection and slicing 11. Collect vibratome blades from storage, immediately wash off acetone with water, and air dry. Alcohol sterilize and air dry shortly before use.12. Cut off the bottom of a 50-ml Falcon tube at the 35-ml mark to utilize as a chamber for the agarose brain block.13. Prepare a fresh tube of 2% (w/v) low-melting-point agarose in slicing solution as in step 6 and place in the 42°C water bath.This will be enough for two animals and should remain liquid in the tube until needed for the second animal.14. Turn on the laminar flow cabinet and clean the surface using 70% ethanol. Sterilize the hood with UV irradiation for ∼20 min prior to use.15. Clear a workspace on a bench near the hood to use for decapitation. Set up guillotine and surgical scissors.16. Sterilize and place the cyanoacrylate glue, 100-mm plastic Petri dish, 100-mm filter paper, three 24-well plates, ice bucket with ice, the cut Falcon tube top with lid (step 12), two additional 50-ml Falcon tubes, vibratome razor blade, small straight dissecting scissors, straight fine forceps, polished microspatula, industrial razor blade, 1-ml syringe with 25-G, 5/8-in. needle, sable paint brush, paper towel, 25-ml serological pipet with controller, and 1000-μl pipet tips and pipetter in the sterilized biosafety cabinet.Again, all items must be sterilized before being placed in the hood.

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NETO1 Regulates Postsynaptic Kainate Receptors in CA3 Interneurons During Circuit Maturation

et al. 2019

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Kainate type ionotropic glutamate receptors (KARs) are expressed in hippocampal interneurons and regulate interneuron excitability and GABAergic transmission. Neuropilin tolloid-like proteins (NETO1 and NETO2) act as KAR auxiliary subunits; however, their significance for various functions of KARs in GABAergic interneurons is not fully understood. Here we show that NETO1, but not NETO2, is necessary for dendritic delivery of KAR subunits and, consequently, for formation of KAR-containing synapses in cultured GABAergic neurons. Accordingly, electrophysiological analysis of neonatal CA3 stratum radiatum interneurons revealed impaired postsynaptic and metabotropic KAR signaling in Neto1 knockouts, while a subpopulation of ionotropic KARs in the somatodendritic compartment remained functional. Loss of NETO1/KAR signaling had no significant effect on development of αamino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and N-methyl-D-aspartate (NMDA)-receptor-mediated glutamatergic transmission in CA3 interneurons, contrasting the synaptogenic role proposed for KARs in principal cells. Furthermore, loss of NETO1 had no effect on excitability and characteristic spontaneous network bursts in the immature CA3 circuitry. However, we find that NETO1 is critical for kainate-dependent modulation of network bursts and GABAergic transmission in the hippocampus already during the first week of life. Our results provide the first description of NETO1-dependent subcellular targeting of KAR subunits in GABAergic neurons and indicate that endogenous NETO1 is required for formation of KAR-containing synapses in interneurons. Since aberrant KAR-mediated excitability is implicated in certain forms of epilepsy, NETO1 represents a potential therapeutic target for treatment of both adult and early life seizures.

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Development of Cortical Pyramidal Cell and Interneuronal Dendrites: a Role for Kainate Receptor Subunits and NETO1

Cited by 24 publications

References 81 publications

GluN2B but Not GluN2A for Basal Dendritic Growth of Cortical Pyramidal Neurons

GluN2B but Not GluN2A for Basal Dendritic Growth of Cortical Pyramidal Neurons

Long‐Lived Organotypic Slice Culture Model of the Rat Basolateral Amygdala

NETO1 Regulates Postsynaptic Kainate Receptors in CA3 Interneurons During Circuit Maturation

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