Development of digestive enzyme activity in spotted rose snapper, Lutjanus guttatus (Steindachner, 1869) larvae

Moguel-Hernández, Ivette; Peña, Renato; Nolasco-Soria, Héctor; Dumas, Silvie; Zavala‐Leal, Iram

doi:10.1007/s10695-013-9890-7

Cited by 20 publications

(24 citation statements)

References 47 publications

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“…The activity of trypsin could be detected before mouth opening of stinger larvae, and similar result was observed in several species, such as Senegal sole (Martinez, Moyano, Fern andez-D ıaz & Y ufera, 1999), Asian seabass (Srichanun et al, 2013), spotted rose snapper (Galaviz et al, 2012;Moguel-Hern andez et al, 2014). The result of histological analysis revealed that the pancreas has already formed and pancreas cells began to differentiate at 3 dph along with the increasing of eosinophilic cells, indicating the ability of stinger larvae to digest the exogenous feeds.…”

Section: Discussionsupporting

confidence: 77%

“…Then, gastric gland could be observed at 15 dph, coinciding with the remarkably increasing pepsin activity, indicating the improvement of digestive function (Peña, Dumas, Villalejo-Fuerte & Ortız-Galindo, 2003). The increasing pepsin activities have also been demonstrated with larval development in white seabass (Galaviz, Garc ıa-Gasca, Drawbridge, Alvarez-Gonz alez & L opez, 2011), Asian seabass (Srichanun et al, 2013) and spotted rose snapper (Moguel-Hern andez et al, 2014). The emergence time of gastric gland was different among fish species.…”

Section: Discussionmentioning

confidence: 92%

“…The developmental process of digestive system, particularly the variation of digestive enzyme activities, could provide evidence to apply formulated feeds on larvae (Ma et al., ; Moguel‐Hernández et al., ; Peña et al. 2106).…”

Section: Discussionmentioning

confidence: 99%

“…The ontogenesis of digestive enzymes has been well studied in several species, such as seabass (Walford & Lam, ), Japanese flounder (Kurokawa & Suzuki, ), Senegalese sole (Ribeiro, Sarasquete & Dinis, ), large yellow croaker (Ma et al., ), sharpsnout seabream (Suzer et al., ), spotted rose snapper (Galaviz, García‐Ortega, Gisbert, López & Gasca, ; Moguel‐Hernández, Peña, Nolasco‐Soria, Dumas & Zavala‐Leal, ), Asian seabass (Srichanun, Tantikitti, Utarabhand & Kortner, ) and gilthead seabream (Mata‐Sotres, Moyano, Martínez‐Rodríguez & Yúfera, ). The above studies confirmed the age‐dependent pattern of digestive enzymes.…”

Section: Introductionmentioning

confidence: 99%

“…, large yellow croaker (Ma et al, 2005), sharpsnout seabream (Suzer et al, 2007), spotted rose snapper (Galaviz, Garc ıa-Ortega, Gisbert, L opez & Gasca, 2012;Moguel-Hern andez, Peña, Nolasco-Soria, Dumas & Zavala-Leal, 2014), Asian seabass (Srichanun, Tantikitti, Utarabhand & Kortner, 2013) and gilthead seabream (Mata-Sotres, Moyano, Mart ınez-Rodr ıguez & Y ufera, 2016). The above studies confirmed the age-dependent pattern of digestive enzymes.…”

mentioning

confidence: 99%

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Activities of digestive enzymes and histology of digestive system during larval development of devil stinger (Inimicus japonicus)

Wen

Huang

et al. 2017

Aquac Res

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Devil stinger is a valuable demersal scorpaenid fish while the rearing of stinger larvae still relies on live prey. This study was conducted to illustrate the development of the main digestive enzymes and digestive system during larval development of this species to provide evidence for the application of artificial feeds. Enzymatic and histological assays were conducted from 1 day post hatching (dph) to 36 dph in larvae. The result showed that the selected digestive enzyme activities increased significantly after 15 dph. Specifically, the total trypsin activities increased significantly from 18 dph to 33 dph. The total pepsin and amylase activities increased significantly first and thereafter decreased significantly. The lipase activities followed the similar pattern with trypsin. With regard to the histological study, the stinger larvae open their mouth to first feeding at 3 dph and turned into totally exogenous nutritional stage at 6 dph. In addition, mucous membrane, rich in goblet cells, was widely distributed in oesophagus epithelium at 18 dph. The height and amounts of gastric gland in cardia and main body of the stomach increased gradually with the development of stinger larvae after 15 dph. The intestine length of stinger larvae was short, and goblet cell was abundant in anterior intestine after 12 dph, not the posterior intestine. The ontogeny of liver and pancreas started from newly hatched stage, and the differentiation of liver was prior to pancreas. The above findings would provide evidence for the use of artificial feeds from the larval stage of stinger larvae (at least from 21 dph).

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Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Activities of digestive enzymes and histology of digestive system during larval development of devil stinger (Inimicus japonicus)

Wen

Huang

et al. 2017

Aquac Res

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Changes in digestive enzyme activities during larval development of Chinese loach Paramisgurnus dabryanus (Dabry de Thiersant, 1872)

Zhang

et al. 2015

Fish Physiol Biochem

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The digestive physiology of Chinese loach (Paramisgurnus dabryanus) was studied by assessing the specific and total activities of different pancreatic (trypsin, chymotrypsin, amylase and lipase), gastric (pepsin) and intestinal (alkaline phosphatase and leucine-aminopeptidase) enzymes from hatching to 40 days after hatching (DAH). Larvae were reared at 24.4 ± 0.4 °C and fed with rotifers from mouth opening (4 DAH) to 15 DAH, from 10 to 35 DAH with Cladocera and from 30 to 40 DAH with compound diet. Enzyme activities for trypsin, chymotrypsin, amylase and lipase were detected before the onset of exogenous feeding, indicating that these enzymes were genetically pre-programmed. Most of the pancreatic enzyme specific activities increased until 20 DAH and decreased thereafter. The pepsin activity of Chinese loach was firstly detected at 30 DAH, indicating the appearance of functional gastric gland. Alkaline phosphatase specific activity was detected from hatching onward, showed marked increase and reached the second peak at 20 DAH, while a gradual increase in specific leucine-aminopeptidase activity was observed until the end of the experiment. Accordingly, the larvae of Chinese loach possess a functional digestive system before the onset of exogenous feeding and the digestive capacity gradually increases as development progresses. The abrupt increase in intestinal enzyme activities between 10 and 20 DAH demonstrates onset of juvenile-like digestive mode in Chinese loach larvae. The increase in pepsin activity after 30 DAH indicates the shift from alkaline to acidic digestion in Chinese loach larvae, which may be considered as the onset of weaning.

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Ontogeny changes and weaning effects in gene expression patterns of digestive enzymes and regulatory digestive factors in spotted rose snapper (Lutjanus guttatus) larvae

Moguel-Hernández

Peña

Andrée

et al. 2016

Fish Physiol Biochem

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The study of digestive physiology is an important issue in species that have been introduced in aquaculture like the spotted rose snapper (Lutjanus guttatus). The aims of this study were to describe the expression of digestive enzymes (trypsinogen, chymotrypsinogen, α-amylase, lipoprotein lipase, phospholipase A and pepsinogen) and their relation with orexigenic (neuropeptide Y, NPY) and anorexigenic (cholecystokinin, CCK) factors during the larval development and to evaluate the effect of weaning in their expression. The results showed that the transcripts of all the assayed digestive enzymes, with the exception of pepsinogen, and NPY and CCK were already present in L. guttatus from the hatching stage. The expression of all the enzymes was low during the yolk-sac stage (0-2 days after hatching, DAH), whereas after the onset of exogenous feeding at 2 DAH, their expression increased and fluctuated throughout larval development, which followed a similar pattern as in other marine fish species and reflected changes in different types of food items and the progressive maturation of the digestive system. On the other hand, weaning of L. guttatus larvae from live prey onto a microdiet between 25 and 35 DAH significantly affected the relative expression of most pancreatic digestive enzymes during the first weaning days, whereas chymotrypsinogen 2 and lipoprotein lipase remained stable during this period. At the end of co-feeding, larvae showed similar levels of gene expression regardless of the diet (live prey vs. microdiet), which indicated that larvae of L. guttatus were able to adapt their digestive capacities to the microdiet. In contrast, feeding L. guttatus larvae with live feed or microdiet did not affect the expression of CCK and NPY. The relevance of these findings with regard to current larval rearing procedures of L. guttatus is discussed.

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Development of digestive enzyme activity in spotted rose snapper, Lutjanus guttatus (Steindachner, 1869) larvae

Cited by 20 publications

References 47 publications

Activities of digestive enzymes and histology of digestive system during larval development of devil stinger (Inimicus japonicus)

Activities of digestive enzymes and histology of digestive system during larval development of devil stinger (Inimicus japonicus)

Changes in digestive enzyme activities during larval development of Chinese loach Paramisgurnus dabryanus (Dabry de Thiersant, 1872)

Ontogeny changes and weaning effects in gene expression patterns of digestive enzymes and regulatory digestive factors in spotted rose snapper (Lutjanus guttatus) larvae

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