Development of direct competitive enzyme-linked aptamer assay for determination of dopamine in serum

Park, Hoyoung; Paeng, Insook Rhee

doi:10.1016/j.aca.2010.11.010

Cited by 78 publications

(36 citation statements)

References 25 publications

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“…The authors suggested that a similar 3-dimensional structure as that proposed for the RNA aptamer existed with the DNA aptamer; this was later confirmed using circular dichroism [31]. Building on the work of Park et al, the DNA aptamer was shown to exhibit increased sensitivity and specificity compared to the RNA aptamer in a competitive enzyme-linked aptamer assay (ELAA) for the detection of DA in human serum [32,33]. In this assay, the detection limits of the DNA and RNA aptamers were 3.2 · 10 -12 M and 6.3 · 10 -8 M respectively.…”

Section: Neurotransmitters and Neurotoxinsmentioning

confidence: 88%

“…Botulinum neurotoxin (BoNT) is one of the most devastating neurotoxins RNA [29] Human serum Competitive enzyme-linked apt assay (DR: 50 pM-0.1 mM; LOD: 1.0 pM) [32] DNA [30] Human serum Label-free electrochemical aptasensor (DR: 0.007-90 nM; LOD: 0.00198 nM) [34] RNA [29] Phosphate buffered saline spiked with relevant competitors Specific label-free electrochemical biosensor (DR: 100 nM-5 mM; LOD: 0.1 mM) [35] RNA [29] Tris buffer (pH = 7) spiked with relevant competitors Specific electrochemical biosensor (DR: 100 nM-5 mM; LOD: 50 nM) [36] DNA [30] Dopamine release from PC12 cells under hypoxic conditions Specific label-free nanowire-transistor biosensor (DR in vitro: 10 -11 to 10 -8 M; LOD: 10 -11 M) [37] Apt, aptamer; AuNP, gold nanoparticles; DR, detection range; LOD, limit of detection. known [49].…”

Section: Aptamers For Neurotoxin Detectionmentioning

confidence: 99%

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Aptamers as Promising Molecular Recognition Elements for Diagnostics and Therapeutics in the Central Nervous System

McConnell

Holahan

DeRosa

2014

Nucleic Acid Therapeutics

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Oligonucleotide aptamers are short, synthetic, single-stranded DNA or RNA able to recognize and bind to a multitude of targets ranging from small molecules to cells. Aptamers have emerged as valuable tools for fundamental research, clinical diagnosis, and therapy. Due to their small size, strong target affinity, lack of immunogenicity, and ease of chemical modification, aptamers are an attractive alternative to other molecular recognition elements, such as antibodies. Although it is a challenging environment, the central nervous system and related molecular targets present an exciting potential area for aptamer research. Aptamers hold promise for targeted drug delivery, diagnostics, and therapeutics. Here we review recent advances in aptamer research for neurotransmitter and neurotoxin targets, demyelinating disease and spinal cord injury, cerebrovascular disorders, pathologies related to protein aggregation (Alzheimer's, Parkinson's, and prions), brain cancer (glioblastomas and gliomas), and regulation of receptor function. Challenges and limitations posed by the blood brain barrier are described. Future perspectives for the application of aptamers to the central nervous system are also discussed.

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Section: Neurotransmitters and Neurotoxinsmentioning

confidence: 88%

Section: Aptamers For Neurotoxin Detectionmentioning

confidence: 99%

Aptamers as Promising Molecular Recognition Elements for Diagnostics and Therapeutics in the Central Nervous System

McConnell

Holahan

DeRosa

2014

Nucleic Acid Therapeutics

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“…35,36,48,49 These difficulties have been attributed to the complexity in targeting molecules with low molecular mass and rotatable bonds. 59,60 Recent advances in solution-phase SELEX methods that obviate tethering targets have enabled identification of aptamers that recognize small-molecule, low-epitope targets. 21,61–64 Solution-phase selection and/or the use of the target-modified substrates described herein for SELEX are expected to lead to increased opportunities for high-affinity small-molecule aptamer discovery.…”

Section: Resultsmentioning

confidence: 99%

Aptamer Recognition of Multiplexed Small-Molecule-Functionalized Substrates

Nakatsuka

Cao

Deshayes

et al. 2018

ACS Appl. Mater. Interfaces

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Aptamers are chemically synthesized oligonucleotides or peptides with molecular recognition capabilities. We investigated recognition of substrate-tethered small-molecule targets, using neurotransmitters as examples, and fluorescently labeled DNA aptamers. Substrate regions patterned via microfluidic channels with dopamine or L-tryptophan were selectively recognized by previously identified dopamine or L-tryptophan aptamers, respectively. The on-substrate dissociation constant determined for the dopamine aptamer was comparable to, though slightly greater than the previously determined solution dissociation constant. Using pre-functionalized neurotransmitter-conjugated oligo(ethylene glycol) alkanethiols and microfluidics patterning, we produced multiplexed substrates to capture and to sort aptamers. Substrates patterned with L-DOPA, L-DOPS, and L-5-HTP enabled comparison of the selectivity of the dopamine aptamer for different targets via simultaneous determination of in situ binding constants. Thus, beyond our previous demonstrations of recognition by protein binding partners (i.e., antibodies and G-protein-coupled receptors), strategically optimized small-molecule-functionalized substrates show selective recognition of nucleic acid binding partners. These substrates are useful for side-by-side target comparisons, and future identification and characterization of novel aptamers targeting neurotransmitters or other important small-molecules.

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“…Generally these contain 20 -100 nucleotides in length [25]. The more the randomization of ss nucleic acid sequences provides a wide range of diversity 4 n where n refers for the number of nucleotides that were used for the selection of aptamers [2,6,17]. Libraries containing these nucleotides were flanked by the constant sequences for priming or capturing.…”

Section: Selection Of Aptamersmentioning

confidence: 99%

“…Dedicated biosensors that have high sensitivity, linearity and less reaction time are required for their early detection [1]. Biosensors are analytical devices composed of some biological recognition including the use of biochemical reactions, antibody antigen reaction, electrochemical reactions [1,2]. These responses directly are interfaced to signal transducers which together relate the concentration of an analyte to a measurable response.…”

Section: Introductionmentioning

confidence: 99%

Aptamers—A Promising Approach for Sensing of Biothreats Using Different Bioinformatics Tools

Sharma¹,

Sharma²

2013

SNL

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Intentional release of pathogens or biotoxin against humans, plants, or animals is an impending threat all over the world. Continuous monitoring of environment is required for their detection. These signals can help to distinguish whether the bioattack has occurred or not. Biosensors utilise biological response including different biochemical reactions, antigen antibody reactions, electrochemical reactions, aptameric reactions etc. The currently available biosensors have a limit of detection, specificity and less linearity which affect their sensitivity. Aptamers are single stranded oligonucleotides binding species which are capable of tightly binding to their distinguishing targets. They are evolved from random oligonucleotides pools by using different strategies. These are capable of conscientiously distinguishing their target ligands. They have high sensitivity and a wide range of detection limit. The versatility of nucleic acid based methods allowed for the design of specific aptamer sequences, typically on the order of 10 to 30 base pairs in length, identifying the different biothreat agents in the environment. By using different bioinformatics tools we can design RNA aptamers for toxins of lectin family.

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Development of direct competitive enzyme-linked aptamer assay for determination of dopamine in serum

Cited by 78 publications

References 25 publications

Aptamers as Promising Molecular Recognition Elements for Diagnostics and Therapeutics in the Central Nervous System

Aptamers as Promising Molecular Recognition Elements for Diagnostics and Therapeutics in the Central Nervous System

Aptamer Recognition of Multiplexed Small-Molecule-Functionalized Substrates

Aptamers—A Promising Approach for Sensing of Biothreats Using Different Bioinformatics Tools

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