Development of DNA Barcodes for Major Insect Pest of Cole Crops  in Mid-Hills of Meghalaya

Lalrinfeli, R.; Behere, G. T.; Firake, D. M.; Sharma, Bhagawati; Banerjee, Amrita; Rajesh, T.P.

doi:10.20546/ijcmas.2019.802.091

Cited by 1 publication

(1 citation statement)

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“…Apart from sequences obtained in this study, we searched orthologous cox1 gene sequences from the NCBI nucleotide database and included those with sampling locations in our phylogenetic analysis (Pentinsaari et al, 2014;Hendrich et al, 2015;Nie et al, 2017;Coral şahin et al, 2018;Lalrinfeli et al, 2019) (Supplementary Table 1). Sequences with obvious errors were deleted, and others were used for follow-up analysis.…”

Section: Mitochondrial Gene Amplification Sequencing and Homologous G...mentioning

confidence: 99%

Lack of Genetic Structure Among Populations of Striped Flea Beetle Phyllotreta striolata (Coleoptera: Chrysomelidae) Across Southern China

Yongli³

et al. 2022

Front. Ecol. Evol.

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The striped flea beetle (SFB) Phyllotreta striolata (Fabricius) (Coleoptera: Chrysomelidae) is a major pest of cruciferous vegetables in southern China. The population diversity and genetic structure of SFB are unknown. Here, we assembled a draft genome for the SFB and characterized the distribution of microsatellites. Then, we developed 12 novel microsatellite markers across the genome. We used a segment of the cox1 gene and newly developed microsatellite markers to genotype the genetic diversity of SFB across southern China. There were 44 mitochondrial haplotypes in the SFB populations, with haplotype 2 as the most widespread. The population genetic differentiation was very low, indicated by FST-values (<0.05 except for Guangxi population with other populations based on cox1), high gene flow (4.10 and 44.88 of cox1 and microsatellite, respectively) and Principal Coordinate Analysis across all populations. Mantel test showed genetic distance in SFB was significantly associated with geographic distance based on microsatellites (R2 = 0.2373, P = 0.014) while result based on cox1 (R2 = 0.0365, P = 0.155) showed no significant difference. The phylogenetic analysis did not find any geographically related clades among all haplotypes. Analyses based on microsatellites showed a lack of population genetic structure among all populations. Our study provides a foundation for the future understanding of the ecology and evolution of SFB and its management.

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Section: Mitochondrial Gene Amplification Sequencing and Homologous G...mentioning

confidence: 99%

Lack of Genetic Structure Among Populations of Striped Flea Beetle Phyllotreta striolata (Coleoptera: Chrysomelidae) Across Southern China

Yongli³

et al. 2022

Front. Ecol. Evol.

View full text Add to dashboard Cite

show abstract

Development of DNA Barcodes for Major Insect Pest of Cole Crops in Mid-Hills of Meghalaya

Cited by 1 publication

References 12 publications

Lack of Genetic Structure Among Populations of Striped Flea Beetle Phyllotreta striolata (Coleoptera: Chrysomelidae) Across Southern China

Lack of Genetic Structure Among Populations of Striped Flea Beetle Phyllotreta striolata (Coleoptera: Chrysomelidae) Across Southern China

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