Development of doubled haploids from an elite indica rice hybrid (BS6444G) using anther culture

Naik, Nupur; Rout, Prachitara; Ngangkham, Umakanta; Verma, Ram Lakhan; Katara, Jawahar Lal; Sahoo, Khirod Kumar; Singh, O. N.; Samantaray, Sanghamitra

doi:10.1007/s11240-016-1149-4

Cited by 42 publications

(60 citation statements)

References 25 publications

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“…Many authors have confirmed that cold pre-treatment has a stimulatory effect on androgenic response in several genotypes (Tian et al 2015;Herath et al 2010;Touraev et al 2009). Moreover, a temperature of 10 °C is commonly used as a cold pre-treatment (Rukmini et al 2013;Naik et al 2017). Indeed, Naik et al (2017) described that 7 days at 10 °C resulted in the best callus induction in a japonica cultivar.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, a temperature of 10 °C is commonly used as a cold pre-treatment (Rukmini et al 2013;Naik et al 2017). Indeed, Naik et al (2017) described that 7 days at 10 °C resulted in the best callus induction in a japonica cultivar. Therefore, the changes in cold pre-treatment enhanced the anther culture protocol with a higher rate of induced calluses.…”

Section: Discussionmentioning

confidence: 99%

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Antimitotic and hormone effects on green double haploid plant production through anther culture of Mediterranean japonica rice

Hooghvorst

Ramos-Fuentes

López-Cristofannini

et al. 2018

Plant Cell Tiss Organ Cult

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Rice double haploid (DH) plants are produced mainly through anther culture. In order to improve the anther culture protocol, microspores of two japonica rice genotypes (NRVC980385 and H28) were subjected to three growth regulator combinations and four colchicine treatments on induction medium. In addition, a post anther culture procedure using colchicine or oryzalin was tested to induce double haploid plantlets from haploid plantlets. A cold pre-treatment of microspores for 9 days at 10 °C increased callus induction 50-fold in the NRCV980385 genotype. For both genotypes, 2 mg L −1 2,4-D and 1 mg L −1 kinetin on colchicine-free induction medium gave the best culture responses. The culturability of both genotypes changed on colchicine-supplemented induction media. A high genotype dependency was recorded for callus induction, callus regenerating green plantlets and regeneration of green double haploid plantlets. Colchicine at 300 mg L −1 for 48 h enhanced callus induction 100-fold in H28. Colchicine-supplemented media clearly improved green double haploid plantlet regeneration. We showed that the post-anther culture treatment of haploid plantlets at 500 mg L −1 of colchicine permitted fertile double haploid plantlets to be generated. Finally, an enhanced medium-throughput flow cytometry protocol for rice was tested to analyse all the plantlets from anther and post anther culture.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Antimitotic and hormone effects on green double haploid plant production through anther culture of Mediterranean japonica rice

Hooghvorst

Ramos-Fuentes

López-Cristofannini

et al. 2018

Plant Cell Tiss Organ Cult

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“…In breeding programs, DH production replaces inbred line development through repeated self-pollination, so that major reductions in the time to cultivar release are achieved (Dwivedi et al 2015). Many of the modern barley (Hordeum vulgare L.), rice (Oryza sativa L.) and wheat (Triticum aestivum L.) cultivars are DHs (Devaux and Kasha 2009;Niu et al 2014;Mishra and Rao 2016;Niak et al 2016). When used as parents in hybrid breeding, DHs allow for effective exploitation of hybrid vigour (heterosis) (Birchler et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Inheritance patterns of the response to in vitro doubled haploid induction in perennial ryegrass (Lolium perenne L.)

Begheyn

Roulund

Vangsgaard

et al. 2017

Plant Cell Tiss Organ Cult

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“…Previous reports revealed that generally calli with white colour regenerated albino plantlets, while creamy colour calli regenerated green plantlets (Dewi et al 2004;Safitri et al 2010). Naik et al (2017) *Numbers in the same column followed by the same letters are not significantly different by DMRT at P <0.05. recently reported that only the creamy colour compact calli were able to pass through the regeneration stage into plants, while the watery callus was unable to regenerate. Generally, calli with size more than 4 mm in diameter was gradually browning and died after transferring them into plant regeneration medium, but when those calli eventually made into plant regeneration only albino plantlets were produced (Sasmita et al 2001).…”

Section: Plant Regenerationmentioning

confidence: 98%

Response of Anther Donor Genotypes (F1) from Indica x Indica Crosses to Rice Anther Culture

2019

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<p>Anther culture is one of the in vitro techniques that can be used to accelerate the obtainment of purelines in the form of dihaploid (DH) plants resulted from androgenesis. The objectives of this research were to study response of anther donor genotypes in rice anther culture of F1 populations derived from indica x indica crosses and obtain spontaneous DH lines to be used further for variety development program. Anther donor plant used for the experiment consisted of four kinds of F1 populations derived from single crosses, i.e. IR85/I-5, BioR-81/I-5, Bio-R81/O18, and BioR-82/O-18. The result indicated that kindy of all F1 plants showed similar response to callus induction, but significatly different responses to plant regeneration. Genotypes of anther donor plants (F1) significantly affected the percentage of calli producing green plantlet, but not significantly affected the percentage of calli producing albino plantlet. Anther culturability of anther donor plants (F1) was relatively high, ranged from 2.0 to 7.0%. A total of 73 first generation of spontaneous DH lines (38.8%) from the grew out green plantlets was obtained from this study. The resulted DH lines are readily available to be selected and be used in rice cultivar development program.</p>

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Development of doubled haploids from an elite indica rice hybrid (BS6444G) using anther culture

Cited by 42 publications

References 25 publications

Antimitotic and hormone effects on green double haploid plant production through anther culture of Mediterranean japonica rice

Antimitotic and hormone effects on green double haploid plant production through anther culture of Mediterranean japonica rice

Inheritance patterns of the response to in vitro doubled haploid induction in perennial ryegrass (Lolium perenne L.)

Response of Anther Donor Genotypes (F1) from Indica x Indica Crosses to Rice Anther Culture

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