The strengthening of regulations such as EEXI, EEDI, and CII on ship emissions is underway. Despite their application, objective comparisons of ships are hindered by diverse navigation patterns and varying velocity regulations in different seas and ports. Additionally, a lack of basic data impedes comparisons of the optimal design and objective energy efficiency for ships. To address these issues, representative sailing modes, similar to those in the automobile industry, are needed. However, there is no reference for marine applications. This study introduces a methodology for representative sailing modes using the Markov chain. A hundred candidate sailing modes were created, and representative modes were identified through an evaluation equation. All chi-square values for representative sailing modes are within 1%, indicating significant results. This study’s findings can aid in designing optimized systems for new vessels and computing authorized fuel efficiency for vessels with diverse sailing patterns.