Development of Encapsulation Dehydration

Engelmann, Florent; Arnao, Maria-Teresa Gonzalez; Wu, Yanan; Escobar, Roosevelt

doi:10.1007/978-0-387-72276-4_4

Cited by 57 publications

(34 citation statements)

References 35 publications

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“…In the extreme dehydration of tissues, most or all freezable water is removed from the cells and vitrification of the internal solution occurs during rapid cooling. This avoids lethal intracellular ice crystallization (Engelmann 2008). The DTA results showed that ice nucleation was not observed below a certain WC threshold (Fig.…”

Section: Discussionmentioning

confidence: 95%

Cryopreservation changes the DNA methylation of embryonic axes of Quercus robur and Fagus sylvatica seeds during in vitro culture

Nuc

Marszałek

Pukacki

2016

Trees

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Key message Dehydration and cryo-stress (2196°C) caused a greater increase in the DNA methylation of embryonic axes (EAs) of beech seeds (tolerant to both stresses) than in EAs of oak (sensitive to both stresses). Simultaneously, in vitro survival of cryopreserved beech EAs was also significantly higher than in those of oak. Abstract DNA methylation plays a key role in the regulation of growth and differentiation in plants. The present study focused on global DNA methylation of embryonic axes (EAs) isolated from the seeds of pedunculate oak (Quercus robur L.) and European beech (Fagus sylvatica L.) after cryopreservation. Oak seeds can be labeled recalcitrant, i.e., sensitive to dehydration, while beech seeds fall under the suborthodox category, i.e., tolerant to dehydration. DNA methylation was determined using a specific antibody directed at the methylated cytosine in CpG dinucleotides. The analysis was carried out on DNA isolated from EA tissues and subjected to (1) dehydration (control), (2) a combination of dehydration and plant vitrification solution (PVS3) and (3) dehydration with vitrification PVS3, and rapid cooling in liquid nitrogen (LN 2 , -196°C) at a cooling rate of 32°C s -1 . Such treatments induced global DNA methylation primarily in tolerant EAs from F. sylvatica seeds. The level of global DNA methylation in this material was 30 % higher than in the control, i.e., dehydrated (not frozen) beech EAs. The same treatment in oak EAs did not evoke such changes. After 120 days of tissue growth, we observed substantially more DNA demethylation in the beech tissues after cryopreservation in LN 2 than in the controls or in tissues subjected to vitrification but not cryopreservation. Information on the global level of DNA methylation can be useful in monitoring procedures aimed at increasing the rate of recovery of EAs following cryopreservation.

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Section: Discussionmentioning

confidence: 95%

Cryopreservation changes the DNA methylation of embryonic axes of Quercus robur and Fagus sylvatica seeds during in vitro culture

Nuc

Marszałek

Pukacki

2016

Trees

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“…Some alternatives to vitrification-based techniques were developed, namely encapsulationdehydration strategies (e.g., Engelmann et al, 2008). Encapsulation-dehydration is based on concepts related with artificial seeds.…”

Section: General Principlesmentioning

confidence: 99%

Somatic Embryogenesis and Cryopreservation in Forest Species: The Cork Oak Case Study

Santos¹

2012

Current Frontiers in Cryopreservation

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“…Posteriormente las cuentas son deshidratadas colocándolas en la corriente de aire de una cámara de flujo laminar o en contenedores con gel de sílice seco (González-Arnao y Engelmann 2006), por lo que gran parte del agua congelable es retirada de las células. La vitrificación de las soluciones celulares tiene lugar durante la rápida exposición al NL (Engelmann et al 2008) evitando así la letal cristalización intracelular (Engelmann 1997). Esta técnica ha sido utilizada con éxito para la crioconservación de más de 70 especies diferentes, incluyéndose entre éstas la menta (Towill 1990).…”

Section: Crioconservaciónunclassified

“…Esta técnica ha sido utilizada con éxito para la crioconservación de más de 70 especies diferentes, incluyéndose entre éstas la menta (Towill 1990). El procedimiento, en general, incluye un preacondicionamiento del material inicial, generalmente a bajas temperaturas en el caso de plantas tolerantes al frío, o cultivándolo en altas concentraciones de sacarosa (Engelmann et al 2008). La recuperación de los explantos crioconservados se hace utilizando el calentamiento rápido en un baño de agua caliente, p.e.…”

Section: Crioconservaciónunclassified

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Estudio de la estabilidad genética asociada a los procesos de crioconservación de ápices de menta

Morales¹,

Carolina²

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Development of Encapsulation Dehydration

Cited by 57 publications

References 35 publications

Cryopreservation changes the DNA methylation of embryonic axes of Quercus robur and Fagus sylvatica seeds during in vitro culture

Cryopreservation changes the DNA methylation of embryonic axes of Quercus robur and Fagus sylvatica seeds during in vitro culture

Somatic Embryogenesis and Cryopreservation in Forest Species: The Cork Oak Case Study

Estudio de la estabilidad genética asociada a los procesos de crioconservación de ápices de menta

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