Development of “Field Level” Chromogenic Assay for Aflatoxin M1 Detection in Milk

Abstract

Material and Methods Chemicals and instrumentation AFM 1 procured from Sigma Aldrich (India). Acetone was obtained from Rankem (India). Indoxyl acetate obtained from Hi-media, Mumbai (India). Medias and germinants (tryptone, sucrose) obtained by Hi-media, Mumbai (India). Dextrose procured from Sigma, Mumbai, India. Disks and strips from enzyme screening were obtained from Fluka, Mumbai (India). Antibiotics were procured from Hi-media, Mumbai (India) while detergents and sanitizers from Qualigens and CDH, New Delhi (India). Pesticides (methyl Parathion, chlorpyriphos) and heavy metals (cadmium, copper, lead) were procured from Sigma Aldrich (India). Certified reference materials of heavy metals and pesticides were obtained by Fluka, Mumbai (India). Milk samples were