Development of first linkage map for Silphium integrifolium (Asteraceae) enables identification of sporophytic self-incompatibility locus

Abstract

Silphium integrifolium (Asteraceae) has been identified as a candidate for domestication as a perennial oilseed crop and has a sporophytic self-incompatibility system-the genetic basis of which is not well understood in the Asteraceae. To address this gap, we sought to map the genomic location of the self-recognition locus (S-locus) in this species.• We used a biparental population and genotyping-by-sequencing to create the first genetic linkage map for this species. Then we developed a novel crossing scheme and set of analysis methods in order to infer S-locus genotypes for a subset of these individuals, allowing us to map the trait. Finally, we identified potential gene candidates using synteny analysis with the annual sunflower (Helianthus annuus) genome.• Our linkage map contains 198 SNP markers and resolved into the correct number of linkage groups. We were able to successfully map the S-locus and identify several potential gene candidates in the sunflower syntenic region.• Our method is effective and efficient, allowed us to map the S. integrifolium S-locus using fewer resources than previous studies, and could be readily be applied to other species. Our best gene candidate appears to be worthy of future work in S. integrifolium and other Asteraceae species.

Mapping quantitative trait loci associated with self-(in)compatibility in goji berries (Lycium barbarum)

“…To assess the SI trait in sunflower, manual selfing was performed, and the number of seeds produced per secondary capitula was counted [ 19 ]. In the study of sporophytic SI Silphium integrifolium (Asteraceae), a set of seeds, calculated as the ratio of fertilized to total ovules, above 20% was considered to indicate compatibility [ 43 ]. In this study, four indicators related to the SI were used for each evaluation method, namely, the FR, AFW, CI, and CCI.…”

Increased Invasion Risk of Tagetes minuta L. in China under Climate Change: A Study of the Potential Geographical Distributions

“…Tagetes minuta L. is considered a damaging IAS owing to its high sensitization and the decrease in native biodiversity after its colonization [ 25 ]. In addition, it has a strong reproductive capacity and high tolerance to the environment [ 12 ]. For instance, a study in Nyingchi, Tibet, showed that T. minuta belongs to a generalized pollination system and has successfully used local pollinators for pollination [ 25 ].…”

“…Climate warming will facilitate the spread and establishment of IAPs and change their hierarchies in ecosystems [ 10 ]. Amongst IAPs, Asteraceae is globally invasive owing to its biological characteristics, such as asexual reproduction and short growth periods [ 11 , 12 ], and one of the most problematic weeds in Asia, Oceania, and Africa, with serious ecological impacts and economic losses [ 13 ]. For instance, the invasion of Asteraceae weeds in America has reduced crop and pasture yields on agricultural lands [ 14 ].…”

Assessing phenotypic diversity in silflower (Silphium integrifolium Michx.) to identify traits of interest for domestication selection

“…Selected individuals were dug out of the field in the winter of 2015, and crowns were divided into six or 12 parts then placed into potting mix to overwinter. As S. integrifolium is self‐incompatible (Price, Raduski, et al., 2022), clonal propagation was necessary in order to replicate genotypes in multiple environments.…”