Development of Hearing in the Normal Cba-J Mouse:<i>Correlation of Physiological Observations with Behavioral Responses and with Cochlear Anatomy</i>

Mikaelian, Diran O.; Ruben, Robert J.

doi:10.3109/00016486509124579

Cited by 220 publications

(65 citation statements)

References 5 publications

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“…Consistent with other reports, auditory sensitivity of wild-type mice improves between P16 and 3 weeks of age (Mikaelian and Ruben 1965;Song et al 2006). However, two reduced PMCA2 expression strains, +/dfw i5 and +/dfw 2J , demonstrate a distinct loss of auditory sensitivity at 3 weeks of age with frequency-dependent recovery by 5 weeks of age.…”

Section: Pmca2 As a Component Of Auditory Maturationsupporting

confidence: 78%

“…Mice lacking PMCA2 are completely deaf so the haploinsufficient strain, +/dfw i5 (Watson and Tempel 2013), was used to determine the effect of a reduction of PMCA2 expression on auditory development. Mice begin to hear around post-natal day 12 (P12) and have a mature endocochlear potential by P14 (Mikaelian and Ruben 1965). To ensure consistently detectable auditory brainstem responses, thresholds were assessed first at P16, followed by 3 (P20 or P21), 4, 5, 7, and 9 weeks of age (Fig.…”

Section: Maturation Of Auditory Sensitivity In Normal and Pmca2 Mutanmentioning

confidence: 99%

“…PMCA2 is a neuronal PMCA isoform that is expressed in spiral ganglia neurons which innervate the hair cells of the inner ear, and is abundantly expressed in the stereocilia bundles of outer hair cells Dumont et al 2001;Wood et al 2004;Chen et al 2012). Coordinated auditory responses are not detected in mice until around P12 which is commonly denoted as the "onset of hearing" (Mikaelian and Ruben 1965). Expression of PMCA2 mRNA is detected as early as P0 in the rodent cochlea, and strong expression persists in spiral ganglia and outer hair cells into adulthood (Furuta et al 1998).…”

Section: Introductionmentioning

confidence: 99%

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Changes in Cochlear PMCA2 Expression Correlate with the Maturation of Auditory Sensitivity

Watson

Lies

Minich

et al. 2014

JARO

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The plasma membrane Ca 2+ ATPase 2 (PMCA2) is necessary for auditory transduction and serves as the primary Ca 2+ extrusion mechanism in auditory stereocilia bundles. To date, studies examining PMCA2 in auditory function using mutant mice have focused on the phenotype of late adolescent and adult mice. Here, we focus on the changes of PMCA2 in the maturation of auditory sensitivity by comparing auditory responses to RNA and protein expression levels in haploinsufficient PMCA2 and wild-type mice from P16 into adulthood. Auditory sensitivity in wild-type mice improves between P16 and 3 weeks of age, when it becomes stable through adolescence. In haploinsufficient mice, there are frequency-dependent loss of sensitivity and subsequent recovery of thresholds between P16 and adulthood. RNA analysis demonstrates that α-Atp2b2 transcript levels increase in both wild-type and heterozygous cochleae between P16 and 5 weeks. The increases reported for the α-Atp2b2 transcript type during this stage in development support the requisite usage of this transcript for mature auditory transduction. PMCA2 expression also increases in wild-type cochleae between P16 and 5 weeks suggesting that this critical auditory protein may be involved in normal maturation of auditory sensitivity after the onset of hearing. We also characterize expression levels of two long noncoding RNA genes, Gm15082 (lnc82) and Gm15083 (lnc83), which are transcribed on the opposite strand in the 5′ region of Atp2b2 and propose that the lnc83 transcript may be involved in regulating α-Atp2b2 expression.

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Section: Pmca2 As a Component Of Auditory Maturationsupporting

confidence: 78%

Section: Maturation Of Auditory Sensitivity In Normal and Pmca2 Mutanmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Changes in Cochlear PMCA2 Expression Correlate with the Maturation of Auditory Sensitivity

Watson

Lies

Minich

et al. 2014

JARO

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“…Rather, deafness results from the combination of physical occlusion of the external ear canal, poor ossicular transduction in the middle ear, underdeveloped mechanics in the organ of Corti, immature ionic composition in the endolymph, and absence of the endocochlear potential (Bosher and Warren 1971;Anniko and Wroblewski 1986;Woolf and Ryan 1988;Rybak et al 1992;Geal-Dor et al 1993;Abe et al 2007). The time of hearing onset for some common experimental animals is embryonic day 16 (E16) in chicken (Jones et al 2006), postnatal day (P) 10-12 in mouse (Mikaelian and Ruben 1965;Ehret 1983), P11-13 in rat (Uziel et al 1981;Ehret 1983;Geal-Dor et al 1993), P10 in cat (Walsh and McGee 1987), and P12 in gerbil (Woolf and Ryan 1984), with the day of birth defined as P0.…”

Section: Introductionmentioning

confidence: 99%

Spontaneous activity in the developing auditory system

2014

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Spontaneous electrical activity is a common feature of sensory systems during early development. This sensory-independent neuronal activity has been implicated in promoting their survival and maturation, as well as growth and refinement of their projections to yield circuits that can rapidly extract information about the external world. Periodic bursts of action potentials occur in auditory neurons of mammals before hearing onset. This activity is induced by inner hair cells (IHCs) within the developing cochlea, which establish functional connections with spiral ganglion neurons (SGNs) several weeks before they are capable of detecting external sounds. During this pre-hearing period, IHCs fire periodic bursts of Ca(2+) action potentials that excite SGNs, triggering brief but intense periods of activity that pass through auditory centers of the brain. Although spontaneous activity requires input from IHCs, there is ongoing debate about whether IHCs are intrinsically active and their firing periodically interrupted by external inhibitory input (IHC-inhibition model), or are intrinsically silent and their firing periodically promoted by an external excitatory stimulus (IHC-excitation model). There is accumulating evidence that inner supporting cells in Kölliker's organ spontaneously release ATP during this time, which can induce bursts of Ca(2+) spikes in IHCs that recapitulate many features of auditory neuron activity observed in vivo. Nevertheless, the role of supporting cells in this process remains to be established in vivo. A greater understanding of the molecular mechanisms responsible for generating IHC activity in the developing cochlea will help reveal how these events contribute to the maturation of nascent auditory circuits.

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“…In the cochlea, the 2nd postnatal week is characterized morphologically by the in growth of the efferent nerve system, the final morphological maturation of the stereociliary pattern and electrophysiologically by the onset and functional maturation of dif ferent cochlear potentials [18][19][20]. A major myelination of spiral ganglion cells and of myelinated nerve fibres also occurs during the 2nd postnatal week [16].…”

Section: Discussionmentioning

confidence: 99%

Maturation of Inner Ear Ganglion Cells

Anniko

1988

ORL

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Morphometric analyses of the cross-sectional areas were performed on spiral ganglion and vestibular ganglion cells in the CBA/CBA mouse from the 16th gestational day up to the age of 1 month. Vestibular ganglion cells undergo a period of rapid growth between the 4th and 12th postnatal day. Spiral ganglion cells revealed a rapid increase in cross-sectional area during the latter part of the 2nd postnatal week. Increases in cell volume could not be correlated to changes in individual morphological or electrophysiological events, but probably reflect adaptation to function. In 1-month-old animals, the cross-sectional area of both vestibular and spiral ganglion cells had decreased as compared with the 2nd postnatal week.

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Development of Hearing in the Normal Cba-J Mouse:Correlation of Physiological Observations with Behavioral Responses and with Cochlear Anatomy

Cited by 220 publications

References 5 publications

Changes in Cochlear PMCA2 Expression Correlate with the Maturation of Auditory Sensitivity

Changes in Cochlear PMCA2 Expression Correlate with the Maturation of Auditory Sensitivity

Spontaneous activity in the developing auditory system

Maturation of Inner Ear Ganglion Cells

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