Development of high-throughput multi-residue method for non-steroidal anti-inflammatory drugs monitoring in swine muscle by LC-MS/MS

Abstract

A reliable and simple method for the detection and quantification of residues of 14 non-steroidal anti-inflammatory drugs and a metamizole metabolite in swine muscle was developed using liquid chromatography-electrospray ionisation-tandem mass spectrometry (LC-ESI-MS/MS). The samples were extracted with acetonitrile (ACN) in solid-liquid extraction followed by a low-temperature partitioning (LLE-LTP) process at -20 ± 2°C. After evaporation to dryness, the residue was reconstituted with hexane and a mixture of water:acetonitrile (1:1). LC separation was achieved on a reversed-phase (RP18) column with gradient elution using water (phase A) and ACN (phase B) both containing 1 mmol l(-)(1) ammonium acetate (NH4COO) with 0.025% acetic acid. Analysis was carried out on a triple-quadrupole tandem mass spectrometer (LC-MS/MS) in multiple reaction monitoring mode using an electrospray interface in negative and positive mode in a single run. Method validation was performed according to the criteria of Commission Decision No. 2002/657/EC. The matrix effect and linearity were evaluated. Decision limit (CCα), detection capability (CCβ), accuracy and repeatability of the method are also reported. The proposed method proved to be simple, easy and adequate for high-throughput analysis and was applied to routine analysis by the Brazilian Ministry of Agriculture, Livestock and Food Supply.

Pharmacokinetic Profiles of Metamizole Metabolites after Intramuscular and Intravenous Administration in Healthy Arabian Horses

“…The analytical merits such as correlation factor (r) ranges, accuracies, limit of detections (LOD), limit of quantifications (LOQ), precisions and recoveries were validated according to published guidelines (Castilhos et al, 2016; Gyenge‐Szabó et al, 2014).…”

Polycaprolactone Composite Micro/Nanofibrous Material as an Alternative to Restricted Access Media for Direct Extraction and Separation of Non-Steroidal Anti-Inflammatory Drugs from Human Serum Using Column-Switching Chromatography

“…Their determination as a part of rational pharmacotherapy that aims to reduce risks and achieve disease treatment goals is less common. The levels are specifically monitored either in patients with an impaired renal function when the insufficient excretion occurs or when non-compliance is suspected [ 1 , 2 ]. The pharmaceutical industry is another sector where NSAID are determined in pharmaceutical formulations to ensure safety and effectiveness of drugs [ 3 ].…”

Application of gas and liquid chromatography coupled to time‐of‐flight mass spectrometry in pesticides: Multiresidue analysis

“…The high sensitivity and selectivity achieved using GC–MS/MS and LC–MS/MS are subject to some limitations regarding multiclass analysis, especially when the method is applied to a large number of compounds (Anagnostopoulos & Miliadis, ; Castilhos, Barreto, Meneghini, & Bergold, ; Shendy, Al‐Ghobashy, Gad Alla, & Lotfy, ). In GC–MS/MS and LC–MS/MS methods, the acquisition time of each transition restricts the number of target analytes that can be scanned.…”