Development of Highly Validated SNP Markers for Genetics Analyses of Chestnut Species.

Larue, Clément; Guichoux, Erwan; Laurent, Benoit; Barreneche, Teresa; Robin, Cécile; Massot, Marie; Delcamp, Adline; Petit, Rémy

doi:10.21203/rs.3.rs-314998/v1

2021

DOI: 10.21203/rs.3.rs-314998/v1

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Development of Highly Validated SNP Markers for Genetics Analyses of Chestnut Species.

Clément Larue

Erwan Guichoux

Benoit Laurent

et al.

Abstract: To better study and manage chestnut trees and species, we identified nuclear single nucleotide polymorphism (SNP) markers using restriction-associated DNA sequencing. Out of 343 loci tested, 68 SNP markers were selected that withhold stringent quality criteria such as quasi-systematic amplification across species and Mendelian segregation in both purebred and hybrid individuals. They provide sufficient power for species, hybrids and backcross characterization as well as for clonal identification, as shown by a… Show more

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An intensive study plot to investigate chestnut tree reproduction

Larue

Barreneche

Petit

2021

Annals of Forest Science

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Key message Pollination is a key step for fruit production. To provide a tool for future in-depth analysis of pollination in chestnut, we describe in detail a chestnut orchard (location, genotype, phenotype and seed-set of all trees). Context Chestnuts, which are insect-pollinated trees, have been massively planted around the world for nut production. Orchards are planted with clonal varieties selected from crosses between the European chestnuts (Castanea sativa) and Japanese chestnuts (C. crenata) or Chinese chestnuts (C. mollissima) because these two last species are tolerant to blight and ink diseases. Aims To characterize chestnut genetic resources and accurately model male and female fitness as well as pollen exchanges in orchards, we characterized all chestnuts of the INRAE chestnut germplasm collection located near Bordeaux (France). Methods All chestnut trees were geolocated and genotyped using 79 SNP and 98 SSR loci. We scored their flowering phenology using chestnut BBCH scale and precisely described their phenotype (height, diameter a breast height (DBH), canopy diameter…), their capacity to produce pollen (flower type, catkins length…) and their fruit production (number of burrs, seed-set…). Results We geolocated 275 trees and genotyped 273 of them. We identified 115 unique genotypes and assigned each genotype to species. To assess phenology, we evaluated 244 trees twice a week, for 6 weeks from early June to mid-July. We also described tree phenotypes with 11 variables, pollen production with 5 variables and fruit production with 3 variables. All measures were recorded in 2018 except seed set that was measured two consecutive years, in 2018 and 2019. Conclusion The data collected is very detailed and allows modelling precisely pollen exchanges between trees. Parts of this data have been successfully published in scientific articles. Data are available at: https://data.inrae.fr/dataset.xhtml?persistentId=doi:10.15454/GSJSWW Associated metadata are available at:https://metadata-afs.nancy.inra.fr/geonetwork/srv/fre/catalog.search#/metadata/02c5ca07-1536-4f89-9a0c-9e8d44a91287

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An intensive study plot to investigate chestnut tree reproduction

Larue

Barreneche

Petit

2021

Annals of Forest Science

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Development of Highly Validated SNP Markers for Genetics Analyses of Chestnut Species.

Cited by 1 publication

References 28 publications

An intensive study plot to investigate chestnut tree reproduction

An intensive study plot to investigate chestnut tree reproduction

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