Development of immune-affinity 96 spots monolith array for multiple mycotoxins detection in food samples

Li, Li; Xia, Liru; Zhao, Yadong; Wang, Heye

doi:10.1016/j.jchromb.2016.07.012

Cited by 16 publications

(4 citation statements)

References 31 publications

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“…It has also been established that the ingestion of low concentrations of the T-2 toxin alters TLR activation by decreasing the pattern recognition of pathogens, thus interfering with the initiation of inflammatory immune responses against bacteria and viruses [ 84 ]. The development of the immune-affinity 96-spot monolith array and high-sensitive chemiluminescent immunoassay investigation methods are highly promising means of detecting multiple mycotoxins in food samples [ 85 , 86 ].…”

Section: Determination Of the T-2 Toxin: Methods And Techniquesmentioning

confidence: 99%

T-2 mycotoxin: toxicological effects and decontamination strategies

et al. 2017

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Mycotoxins are highly diverse secondary metabolites produced in nature by a wide variety of fungus which causes food contamination, resulting in mycotoxicosis in animals and humans. In particular, trichothecenes mycotoxin produced by genus fusarium is agriculturally more important worldwide due to the potential health hazards they pose. It is mainly metabolized and eliminated after ingestion, yielding more than 20 metabolites with the hydroxy trichothecenes-2 toxin being the major metabolite. Trichothecene is hazardously intoxicating due to their additional potential to be topically absorbed, and their metabolites affect the gastrointestinal tract, skin, kidney, liver, and immune and hematopoietic progenitor cellular systems. Sensitivity to this type of toxin varying from dairy cattle to pigs, with the most sensitive endpoints being neural, reproductive, immunological and hematological effects. The mechanism of action mainly consists of the inhibition of protein synthesis and oxidative damage to cells followed by the disruption of nucleic acid synthesis and ensuing apoptosis. In this review, the possible hazards, historical significance, toxicokinetics, and the genotoxic and cytotoxic effects along with regulatory guidelines and recommendations pertaining to the trichothecene mycotoxin are discussed. Furthermore, various techniques utilized for toxin determination, pathophysiology, prophylaxis and treatment using herbal antioxidant compounds and regulatory guidelines and recommendations are reviewed. The prospects of the trichothecene as potential hazardous agents, decontamination strategies and future perspectives along with plausible therapeutic uses are comprehensively described.

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Section: Determination Of the T-2 Toxin: Methods And Techniquesmentioning

confidence: 99%

T-2 mycotoxin: toxicological effects and decontamination strategies

et al. 2017

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“…The limit of detection (LOD) was calculated to be 0.15 ng mL −1 (signal-to-noise = 3), demonstrating a wide linear response and compatible detection sensitivity toward FB 1 in comparison with the protocols reported previously ( Table 1 ). In particular, the proposed method exhibited relatively low LOD over the antibody-based immunosensors and other fluorescent aptasensors [ 9 , 20 , 25 , 37 , 38 , 39 , 40 , 41 , 42 ]. Additionally, it is well known that the production and preparation of antibodies has a high cost and a long period.…”

Section: Resultsmentioning

confidence: 99%

Nuclease Triggered “Signal-On” and Amplified Fluorescent Sensing of Fumonisin B1 Incorporating Graphene Oxide and Specific Aptamer

Guo

Qiao

Zhang

et al. 2022

IJMS

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Remarkable advancements have been achieved in the development of rapid analytic techniques toward fumonisin B1 (FB1) monitoring and even trace levels for food safety in recent years. However, the point-of-care testing for quantitative and accurate FB1 determination is still challenging. Herein, an innovative aptasensor was established to monitor FB1 by utilizing graphene oxide (GO) and nuclease-triggered signal enhancement. GO can be utilized as a fluorescence quenching agent toward a fluorophore-modified aptamer, and even as a protectant of the aptamer from nuclease cleavage for subsequent target cycling and signal amplification detection. This proposed sensing strategy exhibited a good linearity for FB1 determination in the dynamic range from 0.5 to 20 ng mL−1 with a good correlation of R2 = 0.995. Its limit of detection was established at 0.15 ng mL−1 (S/N = 3), which was significantly lower than the legal requirements by three orders of magnitude. The interferent study demonstrated that the introduced aptasensor possessed high selectivity for FB1. Moreover, the aptasensor was successfully applied to the detection of wheat flour samples, and the results were consistent with the classical ELISA method. The rapid response, sensitive and selective analysis, and reliable results of this sensing platform offer a promising opportunity for food mycotoxin control in point-of-care testing.

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“…The formation of mycotoxins depends not only on the fungal strain but also on environmental conditions, and the reasons for the low concentration of these fungal metabolites in the tested edible oils may be associated with low fungal contamination of the raw material. There is a set maximum concentration for mycotoxin contamination in some foods (Li et al., 2016 ; Nabizadeh et al., 2018 ). However, mycotoxin concentrations in sea buckthorn, flaxseed, hempseed, camelina, and mustard seed oils are not regulated.…”

Section: Resultsmentioning

confidence: 99%

Fatty acid profile and safety aspects of the edible oil prepared by artisans' at small‐scale agricultural companies

Bartkienė

Bartkevičs

Bērziņa

et al. 2021

Food Science & Nutrition

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Nowadays, the popularity of plant-based edible oils is on the rise; they are gaining the interest of consumers because of their functional and health-promoting properties (Vasseghian et al., 2020). Moreover, consumers are increasingly choosing nontraditional oils in search of new food sensory properties and greater functionality.For this reason, edible oils prepared from sea buckthorn, mustard, flaxseed, hempseed, and camelina seeds at small-scale agriculture companies are gaining popularity.Oil prepared from sea buckthorn has a high content of palmitoleic acid; however, oils prepared from seeds and pulp have a different fatty acid (FA) profile (Ciesarová et al., 2020). Sea buckthorn seeds contain 12.5% oil, and the whole fruits, on average, 10% (Zielińska & Nowak, 2017). In addition, sea buckthorn is rich in tocopherols and other bioactive compounds (Olas, 2018;Tudor et al., 2020).

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Development of immune-affinity 96 spots monolith array for multiple mycotoxins detection in food samples

Cited by 16 publications

References 31 publications

T-2 mycotoxin: toxicological effects and decontamination strategies

T-2 mycotoxin: toxicological effects and decontamination strategies

Nuclease Triggered “Signal-On” and Amplified Fluorescent Sensing of Fumonisin B1 Incorporating Graphene Oxide and Specific Aptamer

Fatty acid profile and safety aspects of the edible oil prepared by artisans' at small‐scale agricultural companies

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