Development of inhalable curcumin loaded Nano-in-Microparticles for bronchoscopic photodynamic therapy

Baghdan, Elias; Duse, Lili; Schüer, Julia Janina; Pinnapireddy, Shashank Reddy; Pourasghar, Marcel; Schäfer, Jens; Schneider, Marc; Bakowsky, Udo

doi:10.1016/j.ejps.2019.02.025

Cited by 36 publications

(24 citation statements)

References 48 publications

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“…There are many different photosensitisers that have been tested in studies, but only very few have reached the stage of advanced human clinical trials or approval for clinical use [ 14 , 16 ]. One such photosensitiser with a huge therapeutic potential is curcumin [ 17 , 18 , 19 ]. It is a polyphenol extracted from the rhizomes of turmeric ( Curcuma longa ), naturally occurring in south and southeast Asia [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

“…This plays an important role in membrane integrity and thus the ability to successfully deliver the active ingredient. Curcumin was chosen as it is a commonly used photosensitizer with a well-known impact [ 14 , 17 , 30 ]. Additionally, the suitability of two different nebulisation techniques, vibrating-mesh and air-jet, was tested and compared for the nebulisation of liposomes in small amounts.…”

Section: Introductionmentioning

confidence: 99%

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Improvement of Pulmonary Photodynamic Therapy: Nebulisation of Curcumin-Loaded Tetraether Liposomes

et al. 2021

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Lung cancer is one of the most common causes for a high number of cancer related mortalities worldwide. Therefore, it is important to improve the therapy by finding new targets and developing convenient therapies. One of these novel non-invasive strategies is the combination of pulmonary delivered tetraether liposomes and photodynamic therapy. In this study, liposomal model formulations containing the photosensitiser curcumin were nebulised via two different technologies, vibrating-mesh nebulisation and air-jet nebulisation, and compared with each other. Particle size and ζ-potential of the liposomes were investigated using dynamic light scattering and laser Doppler anemometry, respectively. Furthermore, atomic force microscopy and transmission electron microscopy were used to determine the morphological characteristics. Using a twin glass impinger, suitable aerodynamic properties were observed, with the fine particle fraction of the aerosols being ≤62.7 ± 1.6%. In vitro irradiation experiments on lung carcinoma cells (A549) revealed an excellent cytotoxic response of the nebulised liposomes in which the stabilisation of the lipid bilayer was the determining factor. Internalisation of nebulised curcumin-loaded liposomes was visualised utilising confocal laser scanning microscopy. Based on these results, the pulmonary application of curcumin-loaded tetraether liposomes can be considered as a promising approach for the photodynamic therapy against lung cancer.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Improvement of Pulmonary Photodynamic Therapy: Nebulisation of Curcumin-Loaded Tetraether Liposomes

et al. 2021

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“…Compared to other conventional treatments like radiotherapy, surgery, and chemotherapy, it is more selective and relatively less toxic with less or no damage to normal cells. PDT is comparatively safe and minimally invasive [5]. Three individual components, namely the light source of appropriate wavelength, oxygen (O 2 ) and light-sensitive chemical agents known as photosensitizers are essential for PDT.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Phthalocyanine Mediated Photodynamic Therapy by Catechin on Lung Cancer Cells

2020

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Worldwide, lung cancer remains one of the leading cancers with increasing mortality rates. Though chemotherapy for lung cancer is effective, it is always accompanied by unavoidable and grave side effects. Photodynamic therapy (PDT), using novel photosensitizers, is an advanced treatment method with relatively few side effects. Plant products are emerging as potent photosensitizers (PSs). The dose-dependent effect of Catechin (CA) (20–100 µM) on cellular morphological changes, cell viability, cytotoxicity, proliferation, DNA damage and apoptosis were studied on A549 adenocarcinoma alveolar basal epithelial cells. The effect of CA, along with Zinc phthalocyanine PS at 680 nm and 5 J/cm2 fluency was also studied. As the doses of CA increased, the results showed a pattern of increased cytotoxicity, accompanied by decreased cell viability and proliferation in A549 cells. Also, at 52 µM (IC50), CA in combination with PS significantly increased the cytotoxicity, DNA damage, and apoptosis, as compared to control and PS alone, treated cells in PDT experiments. These findings leave a possible thread that CA can be used in the application of phyto-photodynamic therapy of cancer in future.

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“…Despite its numerous therapeutic effects, the clinical benefits of curcumin are limited due to poor solubility, rapid metabolism, and systemic elimination of the native compound [24]. These limitations can be overcome by using different drug carriers to improve curcumin solubility [25][26][27]. Among such drug carriers, liposomes have been broadly studied showing promising therapeutic effects [28,29].…”

Section: Introductionmentioning

confidence: 99%

Sensitivity of Papilloma Virus-Associated Cell Lines to Photodynamic Therapy with Curcumin-Loaded Liposomes

Ambreen

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Tariq

et al. 2020

Cancers

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Photodynamic therapy (PDT) is a minimally invasive therapeutic approach used in the treatment of various medical conditions and cancerous diseases, involving light, a photosensitizing substance, and oxygen. Curcumin, a naturally occurring compound, carries antitumor activities and potentially could be exploited as a photosensitizer in PDT. Only little is known about liposomal-encapsulated curcumin that could help in increasing the efficacy, stability, and bioavailability of this compound. This study investigates the in vitro effects of curcumin-loaded liposomes in combination with PDT. Three papilloma virus-associated cell lines were treated with curcumin-loaded liposomes corresponding to a curcumin concentration of 0–100 µmol/L for 4 h followed by illumination at 457 nm (blue) for 45, 136, and 227 s at a fluence of 220.2 W/m2 (100 mA) corresponding to 1, 3 and 5 J·cm−2. After 24 h, the biological outcome of the treatment was assessed with the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), SYTO9/PI (propidium iodide), Annexin V-FITC (fluorescein isothiocyanate)/PI, clonogenic survival, and scratch (wound closure) assays. Photoactivation of curcumin-loaded liposomes led to a significant reduction in colony formation and migratory abilities, as well as to an increase in tumor cell death. The results point to the combination of curcumin-loaded liposomes with PDT as a potentially useful tool for the treatment of papillomavirus-associated malignancies.

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Development of inhalable curcumin loaded Nano-in-Microparticles for bronchoscopic photodynamic therapy

Cited by 36 publications

References 48 publications

Improvement of Pulmonary Photodynamic Therapy: Nebulisation of Curcumin-Loaded Tetraether Liposomes

Improvement of Pulmonary Photodynamic Therapy: Nebulisation of Curcumin-Loaded Tetraether Liposomes

Enhancement of Phthalocyanine Mediated Photodynamic Therapy by Catechin on Lung Cancer Cells

Sensitivity of Papilloma Virus-Associated Cell Lines to Photodynamic Therapy with Curcumin-Loaded Liposomes

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